Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/47720 |
Resumo: | Fundação de Amparo à Pesquisa do Estado de São Paulo to LF and LL (2012/23124-4) and to LL (2017/24832-6) |
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Farias, Leonardo P.Vance, Gillian M.Coulson, Patricia S.Souza, Juliana VitorianoSilva Neto, Almiro Pires daWangwiwatsin, ArpornNeves, Leandro XavierBorges, William CastroMcNicholas, StuartWilson, Keith S.Leite, Luciana C. C.Wilson, R. Alan2021-06-16T17:15:20Z2021-06-16T17:15:20Z2021FARIAS, Leonardo P. et al. Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine. Frontiers in Immunology, v. 11, 3 Mar. 2021.1664-3224https://www.arca.fiocruz.br/handle/icict/4772010.3389/fimmu.2020.624613Fundação de Amparo à Pesquisa do Estado de São Paulo to LF and LL (2012/23124-4) and to LL (2017/24832-6)Fundação Butantan, and by fellowships from FAPESP to JV-S (2012/18.095-5)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) to LLCoordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil CAPES) –Finance Code 001Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil.University of York. York Biomedical Research Institute. York, United Kingdom.University of York. York Biomedical Research Institute. York, United Kingdom.Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil.Wellcome Sanger Institute. Parasite Genomics. Cambridge, United Kingdom.Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Ouro Preto, MG, Brasil.University of York. York Structural Biology Laboratory. York, United Kingdom.University of York. York Structural Biology Laboratory. York, United Kingdom.Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil.University of York. York Biomedical Research Institute. York, United Kingdom.The radiation-attenuated cercarial vaccine remains the gold standard for the induction of protective immunity against Schistosoma mansoni. Furthermore, the protection can be passively transferred to naïve recipient mice from multiply vaccinated donors, especially IFNgR KO mice. We have used such sera versus day 28 infection serum, to screen peptide arrays and identify likely epitopes that mediate the protection. The arrays encompassed 55 secreted or exposed proteins from the alimentary tract and tegument, the principal interfaces with the host bloodstream. The proteins were printed onto glass slides as overlapping 15mer peptides, reacted with primary and secondary antibodies, and reactive regions detected using an Agilent array scanner. Pep Slide Analyzer software provided a numerical value above background for each peptide from which an aggregate score could be derived for a putative epitope. The reactive regions of 26 proteins were mapped onto crystal structures using the CCP4 molecular graphics, to aid selection of peptides with the greatest accessibility and reactivity, prioritizing vaccine over infection serum. A further eight MEG proteins were mapped to regions conserved between family members. The result is a list of priority peptides from 44 proteins for further investigation in multiepitope vaccine constructs and as targets of monoclonal antibodies.engFrontiers MediaSchistosoma mansoniMapeamento de epitoposProteínas de tegumentoTrato digestivoVacina atenuadaAlvos antigênicosSchistosoma mansoniEpitope mappingTegument proteinsAlimentary tractAttenuated vaccineAntigenic targetsEpitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/47720/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALFarias, Leonardo P Epitope....pdfFarias, Leonardo P 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| dc.title.en.fl_str_mv |
Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine |
| title |
Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine |
| spellingShingle |
Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine Farias, Leonardo P. Schistosoma mansoni Mapeamento de epitopos Proteínas de tegumento Trato digestivo Vacina atenuada Alvos antigênicos Schistosoma mansoni Epitope mapping Tegument proteins Alimentary tract Attenuated vaccine Antigenic targets |
| title_short |
Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine |
| title_full |
Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine |
| title_fullStr |
Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine |
| title_full_unstemmed |
Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine |
| title_sort |
Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine |
| author |
Farias, Leonardo P. |
| author_facet |
Farias, Leonardo P. Vance, Gillian M. Coulson, Patricia S. Souza, Juliana Vitoriano Silva Neto, Almiro Pires da Wangwiwatsin, Arporn Neves, Leandro Xavier Borges, William Castro McNicholas, Stuart Wilson, Keith S. Leite, Luciana C. C. Wilson, R. Alan |
| author_role |
author |
| author2 |
Vance, Gillian M. Coulson, Patricia S. Souza, Juliana Vitoriano Silva Neto, Almiro Pires da Wangwiwatsin, Arporn Neves, Leandro Xavier Borges, William Castro McNicholas, Stuart Wilson, Keith S. Leite, Luciana C. C. Wilson, R. Alan |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Farias, Leonardo P. Vance, Gillian M. Coulson, Patricia S. Souza, Juliana Vitoriano Silva Neto, Almiro Pires da Wangwiwatsin, Arporn Neves, Leandro Xavier Borges, William Castro McNicholas, Stuart Wilson, Keith S. Leite, Luciana C. C. Wilson, R. Alan |
| dc.subject.other.pt_BR.fl_str_mv |
Schistosoma mansoni Mapeamento de epitopos Proteínas de tegumento Trato digestivo Vacina atenuada Alvos antigênicos |
| topic |
Schistosoma mansoni Mapeamento de epitopos Proteínas de tegumento Trato digestivo Vacina atenuada Alvos antigênicos Schistosoma mansoni Epitope mapping Tegument proteins Alimentary tract Attenuated vaccine Antigenic targets |
| dc.subject.en.en.fl_str_mv |
Schistosoma mansoni Epitope mapping Tegument proteins Alimentary tract Attenuated vaccine Antigenic targets |
| description |
Fundação de Amparo à Pesquisa do Estado de São Paulo to LF and LL (2012/23124-4) and to LL (2017/24832-6) |
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2021 |
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2021-06-16T17:15:20Z |
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2021-06-16T17:15:20Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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FARIAS, Leonardo P. et al. Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine. Frontiers in Immunology, v. 11, 3 Mar. 2021. |
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https://www.arca.fiocruz.br/handle/icict/47720 |
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1664-3224 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.3389/fimmu.2020.624613 |
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FARIAS, Leonardo P. et al. Epitope mapping of exposed tegument and alimentary tract proteins identifies putative antigenic targets of the attenuated Schistosome vaccine. Frontiers in Immunology, v. 11, 3 Mar. 2021. 1664-3224 10.3389/fimmu.2020.624613 |
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