Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples

Detalhes bibliográficos
Autor(a) principal: Cruz, Heidi Lacerda Alves da
Data de Publicação: 2011
Outros Autores: Montenegro, Rosana de Albuquerque, Lima, Juliana Falcão de Araújo, Poroca, Diogo da Rocha, Lima, Juliana Figueirêdo da Costa, Montenegro, Lílian Maria Lapa, Crovella, Sergio, Schindler, Haiana Charifker
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26056
Resumo: Este trabalho foi apoiado pela FACEPE e pelo PDTIS.
id CRUZ_a7cac5d753ea39236dae58237b28b4e4
oai_identifier_str oai:www.arca.fiocruz.br:icict/26056
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Cruz, Heidi Lacerda Alves daMontenegro, Rosana de AlbuquerqueLima, Juliana Falcão de AraújoPoroca, Diogo da RochaLima, Juliana Figueirêdo da CostaMontenegro, Lílian Maria LapaCrovella, SergioSchindler, Haiana Charifker2018-04-24T13:19:28Z2018-04-24T13:19:28Z2011DA CRUZ, H. L. A. et al. Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples. Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology], v. 42, n. 1, p. 321–329, jan. 2011.1517-8382https://www.arca.fiocruz.br/handle/icict/2605610.1590/S1517-83822011000100041Este trabalho foi apoiado pela FACEPE e pelo PDTIS.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Genética. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Genética. Recife, PE, Brasil / University of Trieste. Genetic Service IRCCS Burlo Garofolo. Trieste, Italy.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.engMycobacterium tuberculosisNested-PCRSangueUrinaDiagnóstico molecularSequência de inserção IS6110Mycobacterium tuberculosisNested-PCRBloodUrineIS6110 insertion sequenceMolecular diagnosisReação em Cadeia da Polimerasenested-PCRTuberculose / diagnósticoTuberculose / sangueColeta de Amostras SanguíneasTuberculose / transmissãoBrasil / epidemiologiaHumanosSensibilidade e especificidadeurinaEvaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/26056/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALEVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND.pdfEVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND.pdfapplication/pdf154505https://www.arca.fiocruz.br/bitstream/icict/26056/2/EVALUATION%20OF%20A%20NESTED-PCR%20FOR%20MYCOBACTERIUM%20TUBERCULOSIS%20DETECTION%20IN%20BLOOD%20AND.pdf1081856297766161be40961e96a4c010MD52TEXTEVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND.pdf.txtEVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND.pdf.txtExtracted texttext/plain35496https://www.arca.fiocruz.br/bitstream/icict/26056/3/EVALUATION%20OF%20A%20NESTED-PCR%20FOR%20MYCOBACTERIUM%20TUBERCULOSIS%20DETECTION%20IN%20BLOOD%20AND.pdf.txt6aed217d1d65605740ef260554166598MD53icict/260562018-08-15 02:58:24.868oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-15T05:58:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
title Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
spellingShingle Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
Cruz, Heidi Lacerda Alves da
Mycobacterium tuberculosis
Nested-PCR
Sangue
Urina
Diagnóstico molecular
Sequência de inserção IS6110
Mycobacterium tuberculosis
Nested-PCR
Blood
Urine
IS6110 insertion sequence
Molecular diagnosis
Reação em Cadeia da Polimerase
nested-PCR
Tuberculose / diagnóstico
Tuberculose / sangue
Coleta de Amostras Sanguíneas
Tuberculose / transmissão
Brasil / epidemiologia
Humanos
Sensibilidade e especificidade
urina
title_short Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
title_full Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
title_fullStr Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
title_full_unstemmed Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
title_sort Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
author Cruz, Heidi Lacerda Alves da
author_facet Cruz, Heidi Lacerda Alves da
Montenegro, Rosana de Albuquerque
Lima, Juliana Falcão de Araújo
Poroca, Diogo da Rocha
Lima, Juliana Figueirêdo da Costa
Montenegro, Lílian Maria Lapa
Crovella, Sergio
Schindler, Haiana Charifker
author_role author
author2 Montenegro, Rosana de Albuquerque
Lima, Juliana Falcão de Araújo
Poroca, Diogo da Rocha
Lima, Juliana Figueirêdo da Costa
Montenegro, Lílian Maria Lapa
Crovella, Sergio
Schindler, Haiana Charifker
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cruz, Heidi Lacerda Alves da
Montenegro, Rosana de Albuquerque
Lima, Juliana Falcão de Araújo
Poroca, Diogo da Rocha
Lima, Juliana Figueirêdo da Costa
Montenegro, Lílian Maria Lapa
Crovella, Sergio
Schindler, Haiana Charifker
dc.subject.other.pt_BR.fl_str_mv Mycobacterium tuberculosis
Nested-PCR
Sangue
Urina
Diagnóstico molecular
Sequência de inserção IS6110
topic Mycobacterium tuberculosis
Nested-PCR
Sangue
Urina
Diagnóstico molecular
Sequência de inserção IS6110
Mycobacterium tuberculosis
Nested-PCR
Blood
Urine
IS6110 insertion sequence
Molecular diagnosis
Reação em Cadeia da Polimerase
nested-PCR
Tuberculose / diagnóstico
Tuberculose / sangue
Coleta de Amostras Sanguíneas
Tuberculose / transmissão
Brasil / epidemiologia
Humanos
Sensibilidade e especificidade
urina
dc.subject.en.pt_BR.fl_str_mv Mycobacterium tuberculosis
Nested-PCR
Blood
Urine
IS6110 insertion sequence
Molecular diagnosis
dc.subject.decs.pt_BR.fl_str_mv Reação em Cadeia da Polimerase
nested-PCR
Tuberculose / diagnóstico
Tuberculose / sangue
Coleta de Amostras Sanguíneas
Tuberculose / transmissão
Brasil / epidemiologia
Humanos
Sensibilidade e especificidade
urina
description Este trabalho foi apoiado pela FACEPE e pelo PDTIS.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2018-04-24T13:19:28Z
dc.date.available.fl_str_mv 2018-04-24T13:19:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DA CRUZ, H. L. A. et al. Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples. Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology], v. 42, n. 1, p. 321–329, jan. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26056
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.doi.none.fl_str_mv 10.1590/S1517-83822011000100041
identifier_str_mv DA CRUZ, H. L. A. et al. Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples. Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology], v. 42, n. 1, p. 321–329, jan. 2011.
1517-8382
10.1590/S1517-83822011000100041
url https://www.arca.fiocruz.br/handle/icict/26056
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/26056/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/26056/2/EVALUATION%20OF%20A%20NESTED-PCR%20FOR%20MYCOBACTERIUM%20TUBERCULOSIS%20DETECTION%20IN%20BLOOD%20AND.pdf
https://www.arca.fiocruz.br/bitstream/icict/26056/3/EVALUATION%20OF%20A%20NESTED-PCR%20FOR%20MYCOBACTERIUM%20TUBERCULOSIS%20DETECTION%20IN%20BLOOD%20AND.pdf.txt
bitstream.checksum.fl_str_mv 447f2497af1ef5cf99b5c07f6fa18dce
1081856297766161be40961e96a4c010
6aed217d1d65605740ef260554166598
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813008892503785472