Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041 |
Resumo: | The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease. |
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Brazilian Journal of Microbiology |
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Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samplesMycobacterium tuberculosisnested-PCRbloodurinemolecular diagnosisIS6110 insertion sequenceThe polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.Sociedade Brasileira de Microbiologia2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041Brazilian Journal of Microbiology v.42 n.1 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000100041info:eu-repo/semantics/openAccessCruz,Heidi Lacerda Alves daMontenegro,Rosana de AlbuquerqueLima,Juliana Falcão de AraújoPoroca,Diogo da RochaLima,Juliana Figueirêdo da CostaMontenegro,Lílian Maria LapaCrovella,SergioSchindler,Haiana Charifkereng2011-01-10T00:00:00Zoai:scielo:S1517-83822011000100041Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2011-01-10T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples |
title |
Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples |
spellingShingle |
Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples Cruz,Heidi Lacerda Alves da Mycobacterium tuberculosis nested-PCR blood urine molecular diagnosis IS6110 insertion sequence |
title_short |
Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples |
title_full |
Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples |
title_fullStr |
Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples |
title_full_unstemmed |
Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples |
title_sort |
Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples |
author |
Cruz,Heidi Lacerda Alves da |
author_facet |
Cruz,Heidi Lacerda Alves da Montenegro,Rosana de Albuquerque Lima,Juliana Falcão de Araújo Poroca,Diogo da Rocha Lima,Juliana Figueirêdo da Costa Montenegro,Lílian Maria Lapa Crovella,Sergio Schindler,Haiana Charifker |
author_role |
author |
author2 |
Montenegro,Rosana de Albuquerque Lima,Juliana Falcão de Araújo Poroca,Diogo da Rocha Lima,Juliana Figueirêdo da Costa Montenegro,Lílian Maria Lapa Crovella,Sergio Schindler,Haiana Charifker |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cruz,Heidi Lacerda Alves da Montenegro,Rosana de Albuquerque Lima,Juliana Falcão de Araújo Poroca,Diogo da Rocha Lima,Juliana Figueirêdo da Costa Montenegro,Lílian Maria Lapa Crovella,Sergio Schindler,Haiana Charifker |
dc.subject.por.fl_str_mv |
Mycobacterium tuberculosis nested-PCR blood urine molecular diagnosis IS6110 insertion sequence |
topic |
Mycobacterium tuberculosis nested-PCR blood urine molecular diagnosis IS6110 insertion sequence |
description |
The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822011000100041 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.42 n.1 2011 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122203505164288 |