Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples

Detalhes bibliográficos
Autor(a) principal: Cruz,Heidi Lacerda Alves da
Data de Publicação: 2011
Outros Autores: Montenegro,Rosana de Albuquerque, Lima,Juliana Falcão de Araújo, Poroca,Diogo da Rocha, Lima,Juliana Figueirêdo da Costa, Montenegro,Lílian Maria Lapa, Crovella,Sergio, Schindler,Haiana Charifker
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041
Resumo: The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.
id SBM-1_7485cd2b90be285a526f1b93c660c463
oai_identifier_str oai:scielo:S1517-83822011000100041
network_acronym_str SBM-1
network_name_str Brazilian Journal of Microbiology
repository_id_str
spelling Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samplesMycobacterium tuberculosisnested-PCRbloodurinemolecular diagnosisIS6110 insertion sequenceThe polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.Sociedade Brasileira de Microbiologia2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041Brazilian Journal of Microbiology v.42 n.1 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000100041info:eu-repo/semantics/openAccessCruz,Heidi Lacerda Alves daMontenegro,Rosana de AlbuquerqueLima,Juliana Falcão de AraújoPoroca,Diogo da RochaLima,Juliana Figueirêdo da CostaMontenegro,Lílian Maria LapaCrovella,SergioSchindler,Haiana Charifkereng2011-01-10T00:00:00Zoai:scielo:S1517-83822011000100041Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2011-01-10T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
title Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
spellingShingle Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
Cruz,Heidi Lacerda Alves da
Mycobacterium tuberculosis
nested-PCR
blood
urine
molecular diagnosis
IS6110 insertion sequence
title_short Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
title_full Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
title_fullStr Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
title_full_unstemmed Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
title_sort Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
author Cruz,Heidi Lacerda Alves da
author_facet Cruz,Heidi Lacerda Alves da
Montenegro,Rosana de Albuquerque
Lima,Juliana Falcão de Araújo
Poroca,Diogo da Rocha
Lima,Juliana Figueirêdo da Costa
Montenegro,Lílian Maria Lapa
Crovella,Sergio
Schindler,Haiana Charifker
author_role author
author2 Montenegro,Rosana de Albuquerque
Lima,Juliana Falcão de Araújo
Poroca,Diogo da Rocha
Lima,Juliana Figueirêdo da Costa
Montenegro,Lílian Maria Lapa
Crovella,Sergio
Schindler,Haiana Charifker
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cruz,Heidi Lacerda Alves da
Montenegro,Rosana de Albuquerque
Lima,Juliana Falcão de Araújo
Poroca,Diogo da Rocha
Lima,Juliana Figueirêdo da Costa
Montenegro,Lílian Maria Lapa
Crovella,Sergio
Schindler,Haiana Charifker
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
nested-PCR
blood
urine
molecular diagnosis
IS6110 insertion sequence
topic Mycobacterium tuberculosis
nested-PCR
blood
urine
molecular diagnosis
IS6110 insertion sequence
description The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822011000100041
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.42 n.1 2011
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
_version_ 1752122203505164288