A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene

Detalhes bibliográficos
Autor(a) principal: Mosimann, Ana Luiza Pamplona
Data de Publicação: 2018
Outros Autores: Siqueira, Mirian Krystel de, Ceole, Lígia Fernanda, Santos, Claudia Nunes Duarte dos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30022
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
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spelling Mosimann, Ana Luiza PamplonaSiqueira, Mirian Krystel deCeole, Lígia FernandaSantos, Claudia Nunes Duarte dos2018-11-14T12:45:44Z2018-11-14T12:45:44Z2018MOSIMANN, Ana Luiza Pamplona et al. A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene. Parasites & Vectors, v. 11, n. 321, p. 1-10, 2018.1756-3305https://www.arca.fiocruz.br/handle/icict/3002210.1186/s13071-018-2907-41756-3305porBMCAura virusTogaviridae InfectionsGene DuplicationInfecciones por TogaviridaeDuplicación de GenAlphavirusInfecções por TogaviridaeDuplicação GênicaA new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. / Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.A new isolate of Aura virus serendipitously discovered as a cell culture contaminant is reported in this manuscript. Aura virus belongs to the family Togaviridae and is classified in the genus Alphavirus. There are only two reports of Aura virus isolation from mosquitoes in the scientific literature, and the existence of a vertebrate host is still unknown. The discovery of this new isolate was based on transmission electron microscopy and nucleic acid amplification through a non-specific RT-PCR amplification protocol followed by sequencing. Then, genetic analysis has shown that the new virus shares a high degree of identity with the previously described isolate (GenBank: AF126284.1). A major difference was observed in the nsP3 gene in which a 234-nucleotide duplication has been identified. Furthermore, a pronounced difference was observed in cell cultures compared to the data available for the previously described isolate. Cell permissiveness and phenotypic characteristics in C6/36, Vero and BHK-21 cells were found to differ from previous reports. This may be due to the genetic differences that have been observed.This way, concluded the genetic and biological characteristics of the new Aura virus isolate are suggestive of viral adaptation to the cell substrate. The development of a cDNA clone will lend a perspective and better understanding of these results as well as open avenues for its use as a biotechnological tool, as seen for other alphaviruses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/30022/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALParasitVectors_Article_29.pdfParasitVectors_Article_29.pdfapplication/pdf3169670https://www.arca.fiocruz.br/bitstream/icict/30022/2/ParasitVectors_Article_29.pdf8e2e78f40ee03e11efcbcda9c8bb300bMD52TEXTParasitVectors_Article_29.pdf.txtParasitVectors_Article_29.pdf.txtExtracted texttext/plain44237https://www.arca.fiocruz.br/bitstream/icict/30022/3/ParasitVectors_Article_29.pdf.txt0ee146aad2068cfb04ec4418fcc17c29MD53icict/300222021-12-23 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dc.title.pt_BR.fl_str_mv A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
title A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
spellingShingle A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
Mosimann, Ana Luiza Pamplona
Aura virus
Togaviridae Infections
Gene Duplication
Infecciones por Togaviridae
Duplicación de Gen
Alphavirus
Infecções por Togaviridae
Duplicação Gênica
title_short A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
title_full A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
title_fullStr A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
title_full_unstemmed A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
title_sort A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
author Mosimann, Ana Luiza Pamplona
author_facet Mosimann, Ana Luiza Pamplona
Siqueira, Mirian Krystel de
Ceole, Lígia Fernanda
Santos, Claudia Nunes Duarte dos
author_role author
author2 Siqueira, Mirian Krystel de
Ceole, Lígia Fernanda
Santos, Claudia Nunes Duarte dos
author2_role author
author
author
dc.contributor.author.fl_str_mv Mosimann, Ana Luiza Pamplona
Siqueira, Mirian Krystel de
Ceole, Lígia Fernanda
Santos, Claudia Nunes Duarte dos
dc.subject.other.pt_BR.fl_str_mv Aura virus
topic Aura virus
Togaviridae Infections
Gene Duplication
Infecciones por Togaviridae
Duplicación de Gen
Alphavirus
Infecções por Togaviridae
Duplicação Gênica
dc.subject.en.pt_BR.fl_str_mv Togaviridae Infections
Gene Duplication
dc.subject.es.pt_BR.fl_str_mv Infecciones por Togaviridae
Duplicación de Gen
dc.subject.decs.pt_BR.fl_str_mv Alphavirus
Infecções por Togaviridae
Duplicação Gênica
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-11-14T12:45:44Z
dc.date.available.fl_str_mv 2018-11-14T12:45:44Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOSIMANN, Ana Luiza Pamplona et al. A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene. Parasites & Vectors, v. 11, n. 321, p. 1-10, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30022
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-018-2907-4
dc.identifier.eissn.none.fl_str_mv 1756-3305
identifier_str_mv MOSIMANN, Ana Luiza Pamplona et al. A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene. Parasites & Vectors, v. 11, n. 321, p. 1-10, 2018.
1756-3305
10.1186/s13071-018-2907-4
url https://www.arca.fiocruz.br/handle/icict/30022
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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