Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/2796 |
Resumo: | Este trabalho foi financiado por concessões do Ministério da Saúde, Fundação Oswaldo Cruz, BioManguinhos e pelo CNPq e FINEP, fundos de pesquisa médica e institutos nacionais de saúde. |
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Croda, Julio Henrique RosaRamos, João G. RMatsunaga, JamesQueiroz, AdrianoHomma, AkiraRiley, Lee WoodlandHaake, David AReis, Mitermayer Galvão dosKo, Albert Icksang2011-08-15T18:03:05Z2011-08-15T18:03:05Z2007CRODA, J. et al. Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis.Journal of Clinical Microbiology, v.45, n.5, p.1528-1534, 2007.0095-1137https://www.arca.fiocruz.br/handle/icict/279610.1128/JCM.02344-06Este trabalho foi financiado por concessões do Ministério da Saúde, Fundação Oswaldo Cruz, BioManguinhos e pelo CNPq e FINEP, fundos de pesquisa médica e institutos nacionais de saúde.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilVeterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California, USA / UCLA School of Medicine. Department of Medicine. Los Angeles, California, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, BrasilUniversity of California. School of Public Health. Berkeley, California, USAVeterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California, USA / UCLA School of Medicine. Department of Medicine. Los Angeles, California, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. Ithaca, New York, USAThere is an urgent need for improved diagnosis of leptospirosis, an emerging infectious disease which imparts a large disease burden in developing countries. We evaluated the use of Leptospira immunoglobulin (Ig)-like (Lig) proteins as a serodiagnostic marker for leptospirosis. Lig proteins have bacterial immunoglobulin- like (Big) tandem repeat domains, a moiety found in virulence factors in other pathogens. Sera from patients identified during urban outbreaks in Brazil reacted strongly with immunoblots of a recombinant fragment comprised of the second to sixth Big domains of LigB from L. interrogans serovar Copenhageni, the principal agent for transmission in this setting. Furthermore, the sera recognized an analogous LigB fragment derived from L. kirschneri serovar Grippotyphosa, a pathogenic serovar which is not endemic to the study area. The immunoblot assay detected anti-LigB IgM antibodies in sera from 92% (95% confidence interval, 85 to 96%) of patients during acute-phase leptospirosis. The assay had a sensitivity of 81% for sera from patients with less than 7 days of illness. Anti-LigB antibodies were found in sera from 57% of the patients who did not have detectable anti-whole-Leptospira responses as detected by IgM enzyme-linked immunosorbent assay and microagglutination test. The specificities of the assay were 93 to 100% and 90 to 97% among sera from healthy individuals and patients with diseases that have clinical presentations that overlap with those of leptospirosis, respectively. These findings indicate that the antibody response to this putative virulence determinant is a sensitive and specific marker for acute infection. The use of this marker may aid the prompt and timely diagnosis required to reduce the high mortality associated with severe forms of the disease.engAmerican Society for MicrobiologyLeptospirose agudaImunoglobulina LeptospiraDiagnóstico SorológicoAcute leptospirosisLeptospira immunoglobulinSerological DiagnosisAnticorpos AntibacterianosProteínas de BactériasMarcadores BiológicosBrasilEstudos de Casos e ControlesHumanosImunoglobulina GImunoglobulina MImunoglobulinasLeptospiraLeptospiroseLeptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCroda_Ramos_Matsunaga_etal.pdfCroda_Ramos_Matsunaga_etal.pdfapplication/pdf96622https://www.arca.fiocruz.br/bitstream/icict/2796/1/Croda_Ramos_Matsunaga_etal.pdff54605feb573942ccf16090965239d8aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2796/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTCroda_Ramos_Matsunaga_etal.pdf.txtCroda_Ramos_Matsunaga_etal.pdf.txtExtracted texttext/plain47316https://www.arca.fiocruz.br/bitstream/icict/2796/5/Croda_Ramos_Matsunaga_etal.pdf.txtddd198ce25024ee4a911ce657b391f70MD55THUMBNAILCroda_Ramos_Matsunaga_etal.pdf.jpgCroda_Ramos_Matsunaga_etal.pdf.jpgGenerated Thumbnailimage/jpeg2165https://www.arca.fiocruz.br/bitstream/icict/2796/4/Croda_Ramos_Matsunaga_etal.pdf.jpg7f89fbe5dd924d18764427730e629ecfMD54icict/27962023-03-15 14:34:09.459oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis |
| title |
Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis |
| spellingShingle |
Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis Croda, Julio Henrique Rosa Leptospirose aguda Imunoglobulina Leptospira Diagnóstico Sorológico Acute leptospirosis Leptospira immunoglobulin Serological Diagnosis Anticorpos Antibacterianos Proteínas de Bactérias Marcadores Biológicos Brasil Estudos de Casos e Controles Humanos Imunoglobulina G Imunoglobulina M Imunoglobulinas Leptospira Leptospirose |
| title_short |
Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis |
| title_full |
Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis |
| title_fullStr |
Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis |
| title_full_unstemmed |
Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis |
| title_sort |
Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis |
| author |
Croda, Julio Henrique Rosa |
| author_facet |
Croda, Julio Henrique Rosa Ramos, João G. R Matsunaga, James Queiroz, Adriano Homma, Akira Riley, Lee Woodland Haake, David A Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| author_role |
author |
| author2 |
Ramos, João G. R Matsunaga, James Queiroz, Adriano Homma, Akira Riley, Lee Woodland Haake, David A Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Croda, Julio Henrique Rosa Ramos, João G. R Matsunaga, James Queiroz, Adriano Homma, Akira Riley, Lee Woodland Haake, David A Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospirose aguda Imunoglobulina Leptospira Diagnóstico Sorológico |
| topic |
Leptospirose aguda Imunoglobulina Leptospira Diagnóstico Sorológico Acute leptospirosis Leptospira immunoglobulin Serological Diagnosis Anticorpos Antibacterianos Proteínas de Bactérias Marcadores Biológicos Brasil Estudos de Casos e Controles Humanos Imunoglobulina G Imunoglobulina M Imunoglobulinas Leptospira Leptospirose |
| dc.subject.en.pt_BR.fl_str_mv |
Acute leptospirosis Leptospira immunoglobulin Serological Diagnosis |
| dc.subject.decs.pt_BR.fl_str_mv |
Anticorpos Antibacterianos Proteínas de Bactérias Marcadores Biológicos Brasil Estudos de Casos e Controles Humanos Imunoglobulina G Imunoglobulina M Imunoglobulinas Leptospira Leptospirose |
| description |
Este trabalho foi financiado por concessões do Ministério da Saúde, Fundação Oswaldo Cruz, BioManguinhos e pelo CNPq e FINEP, fundos de pesquisa médica e institutos nacionais de saúde. |
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2007 |
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2007 |
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2011-08-15T18:03:05Z |
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2011-08-15T18:03:05Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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CRODA, J. et al. Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis.Journal of Clinical Microbiology, v.45, n.5, p.1528-1534, 2007. |
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https://www.arca.fiocruz.br/handle/icict/2796 |
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0095-1137 |
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10.1128/JCM.02344-06 |
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CRODA, J. et al. Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis.Journal of Clinical Microbiology, v.45, n.5, p.1528-1534, 2007. 0095-1137 10.1128/JCM.02344-06 |
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American Society for Microbiology |
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American Society for Microbiology |
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