Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.

Detalhes bibliográficos
Autor(a) principal: Ramirez, Laura Garcia
Data de Publicação: 2014
Outros Autores: Corvo, Laura, Duarte, Mariana Costa, Fumagalli, Miguel Angel Chávez, Valadares, Diogo Garcia, Santos, Diego Moura, Oliveira, Camila Indiani de, Escutia, Marta R, Alonso, Carlos, Bonay, Pedro, Tavares, Carlos Alberto Pereira, Coelho, Eduardo Antonio Ferraz, Soto, Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10387
Resumo: Universidad Autónoma de Madrid.Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
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spelling Ramirez, Laura GarciaCorvo, LauraDuarte, Mariana CostaFumagalli, Miguel Angel ChávezValadares, Diogo GarciaSantos, Diego MouraOliveira, Camila Indiani deEscutia, Marta RAlonso, CarlosBonay, PedroTavares, Carlos Alberto PereiraCoelho, Eduardo Antonio FerrazSoto, Manuel2015-05-18T18:57:28Z2015-05-18T18:57:28Z2014RAMIREZ, L. G. et al. Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis. Parasites & Vectors, v. 7, p. 3, 2014.1756-330510.1186/1756-3305-7-3https://www.arca.fiocruz.br/handle/icict/10387engBioMed CentralCross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad Autónoma de Madrid.Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversidad Autónoma de Madrid.Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversidade Federal de Minas Gerais. Faculdade de Medicina. Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilResearch & Development Department, Laboratorios LETI. Madrid, Spain.Universidad Autónoma de Madrid.Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversidad Autónoma de Madrid.Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil.Universidad Autónoma de Madrid.Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainBACKGROUND: Two Leishmania major ribosomal proteins L3 (LmL3) and L5 (LmL5) have been described as protective molecules against cutaneous leishmaniasis due to infection with L. major and Leishmania braziliensis in BALB/c mice when immunized with a Th1 adjuvant (non-methylated CpG-oligodeoxynucleotides; CpG-ODN). In the present study we analyzed the cross-protective efficacy of an LmL3-LmL5-CpG ODN combined vaccine against infection with Leishmania amazonensis and Leishmania chagasi (syn. Leishmania infantum) the etiologic agents of different clinical forms of human leishmaniasis in South America. METHODS: The combined vaccine was administered subcutaneously to BALB/c mice. After immunization the cellular and humoral responses elicited were analyzed. Mice were independently challenged with L. amazonensis and L. chagasi. The size of the cutaneous lesions caused by the infection with the first species, the parasite loads and the immune response in both infection models were analyzed nine weeks after challenge. RESULTS: Mice vaccinated with the combined vaccine showed a Th1-like response against LmL3 and LmL5. Vaccinated mice were able to delay lesion development due to L. amazonensis infection and to control parasite loads in the site of infection. A reduction of the parasite burden in the lymph nodes draining the site of infection and in the liver and spleen was observed in the vaccinated mice after a subcutaneous infection with L. chagasi. In both models of infection, protection was correlated to parasite antigen-specific production of IFN-γ and down-regulation of parasite-mediated IL-4 and IL-10 responses. CONCLUSIONS: The data presented here demonstrate the potential use of L. major L3 and L5 recombinant ribosomal proteins for the development of vaccines against various Leishmania speciesLeishmania parasitesL3 and L5 ribosomal proteinsBALB/c miceExperimental infectionVaccinesReações Cruzadas/imunologiaLeishmania major/imunologiaVacinas contra Leishmaniose/imunologiaLeishmaniose Cutânea/prevenção & controleProteínas Ribossômicas/imunologiaCélulas Th1/imunologiaAnimaisCitocinas/biossínteseFemininoImunidade CelularImunidade HumoralLeishmaniose Cutânea/imunologiaLeishmaniose Visceral/imunologiaCamundongosOligodesoxirribonucleotídeosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10387/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALRamirez L Cross protective....pdfRamirez L Cross protective....pdfapplication/pdf636908https://www.arca.fiocruz.br/bitstream/icict/10387/2/Ramirez%20%20L%20Cross%20protective....pdf42844314055d54a0e9ea06d321149ef8MD52TEXTRamirez L Cross protective....pdf.txtRamirez L Cross protective....pdf.txtExtracted texttext/plain56325https://www.arca.fiocruz.br/bitstream/icict/10387/3/Ramirez%20%20L%20Cross%20protective....pdf.txt103c304641cfa7a7198028904231e77fMD53icict/103872023-03-15 14:33:10.352oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.
title Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.
spellingShingle Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.
Ramirez, Laura Garcia
Leishmania parasites
L3 and L5 ribosomal proteins
BALB/c mice
Experimental infection
Vaccines
Reações Cruzadas/imunologia
Leishmania major/imunologia
Vacinas contra Leishmaniose/imunologia
Leishmaniose Cutânea/prevenção & controle
Proteínas Ribossômicas/imunologia
Células Th1/imunologia
Animais
Citocinas/biossíntese
Feminino
Imunidade Celular
Imunidade Humoral
Leishmaniose Cutânea/imunologia
Leishmaniose Visceral/imunologia
Camundongos
Oligodesoxirribonucleotídeos
title_short Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.
title_full Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.
title_fullStr Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.
title_full_unstemmed Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.
title_sort Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis.
author Ramirez, Laura Garcia
author_facet Ramirez, Laura Garcia
Corvo, Laura
Duarte, Mariana Costa
Fumagalli, Miguel Angel Chávez
Valadares, Diogo Garcia
Santos, Diego Moura
Oliveira, Camila Indiani de
Escutia, Marta R
Alonso, Carlos
Bonay, Pedro
Tavares, Carlos Alberto Pereira
Coelho, Eduardo Antonio Ferraz
Soto, Manuel
author_role author
author2 Corvo, Laura
Duarte, Mariana Costa
Fumagalli, Miguel Angel Chávez
Valadares, Diogo Garcia
Santos, Diego Moura
Oliveira, Camila Indiani de
Escutia, Marta R
Alonso, Carlos
Bonay, Pedro
Tavares, Carlos Alberto Pereira
Coelho, Eduardo Antonio Ferraz
Soto, Manuel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ramirez, Laura Garcia
Corvo, Laura
Duarte, Mariana Costa
Fumagalli, Miguel Angel Chávez
Valadares, Diogo Garcia
Santos, Diego Moura
Oliveira, Camila Indiani de
Escutia, Marta R
Alonso, Carlos
Bonay, Pedro
Tavares, Carlos Alberto Pereira
Coelho, Eduardo Antonio Ferraz
Soto, Manuel
dc.subject.en.pt_BR.fl_str_mv Leishmania parasites
L3 and L5 ribosomal proteins
BALB/c mice
Experimental infection
Vaccines
topic Leishmania parasites
L3 and L5 ribosomal proteins
BALB/c mice
Experimental infection
Vaccines
Reações Cruzadas/imunologia
Leishmania major/imunologia
Vacinas contra Leishmaniose/imunologia
Leishmaniose Cutânea/prevenção & controle
Proteínas Ribossômicas/imunologia
Células Th1/imunologia
Animais
Citocinas/biossíntese
Feminino
Imunidade Celular
Imunidade Humoral
Leishmaniose Cutânea/imunologia
Leishmaniose Visceral/imunologia
Camundongos
Oligodesoxirribonucleotídeos
dc.subject.decs.pt_BR.fl_str_mv Reações Cruzadas/imunologia
Leishmania major/imunologia
Vacinas contra Leishmaniose/imunologia
Leishmaniose Cutânea/prevenção & controle
Proteínas Ribossômicas/imunologia
Células Th1/imunologia
Animais
Citocinas/biossíntese
Feminino
Imunidade Celular
Imunidade Humoral
Leishmaniose Cutânea/imunologia
Leishmaniose Visceral/imunologia
Camundongos
Oligodesoxirribonucleotídeos
description Universidad Autónoma de Madrid.Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-18T18:57:28Z
dc.date.available.fl_str_mv 2015-05-18T18:57:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMIREZ, L. G. et al. Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis. Parasites & Vectors, v. 7, p. 3, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10387
dc.identifier.issn.none.fl_str_mv 1756-3305
10.1186/1756-3305-7-3
identifier_str_mv RAMIREZ, L. G. et al. Cross-protective effect of a combined L5 plus L3 Leishmania major ribosomal protein based vaccine combined with a Th1 adjuvant in murine cutaneous and visceral leishmaniasis. Parasites & Vectors, v. 7, p. 3, 2014.
1756-3305
10.1186/1756-3305-7-3
url https://www.arca.fiocruz.br/handle/icict/10387
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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