Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35322 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de janeiro, RJ, Brasil. |
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Rocha, Juliana D. B.Nascimento, Michelle T. C.Ricardo, Debora DecoteCôrte-Real, SuzanaMorrot, AlexandreHeise, NortonNunes, Marise P.Previato, José OsvaldoPreviato, Lucia MendonçaReis, George A. dosSaraiva, Elvira M.Lima, Célio G. Freire de2019-09-03T17:25:23Z2019-09-03T17:25:23Z2015ROCHA, Juliana D. B. et al. Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils. Scientific Reports, v. 5, n. 8008, p. 1-10, Jan. 2015.2045-2322https://www.arca.fiocruz.br/handle/icict/3532210.1038/srep08008engNature ResearchPolissacarídeos capsularesCryptococcus neoformansNeutrófilos extracelulares armadilhasNeutrófilos humanosCapsular polysaccharidesCryptococcus neoformansNneutrophil extracellular trapsHuman neutrophilsCapsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophilsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de janeiro, RJ, Brasil.In the present study, we characterized the in vitro modulation of NETs (neutrophil extracellular traps) induced in human neutrophils by the opportunistic fungus Cryptococcus neoformans, evaluating the participation of capsular polysaccharides glucuronoxylomanan (GXM) and glucuronoxylomannogalactan (GXMGal) in this phenomenon. The mutant acapsular strain CAP67 and the capsular polysaccharide GXMGal induced NET production. In contrast, the wild-type strain and the major polysaccharide GXM did not induce NET release. In addition, C. neoformans and the capsular polysaccharide GXM inhibited PMA-induced NET release. Additionally, we observed that the NET-enriched supernatants induced through CAP67 yeasts showed fungicidal activity on the capsular strain, and neutrophil elastase, myeloperoxidase, collagenase and histones were the key components for the induction of NET fungicidal activity. The signaling pathways associated with NET induction through the CAP67 strain were dependent on reactive oxygen species (ROS) and peptidylarginine deiminase-4 (PAD-4). Neither polysaccharide induced ROS production however both molecules blocked the production of ROS through PMA-activated neutrophils. Taken together, the results demonstrate that C. neoformans and the capsular component GXM inhibit the production of NETs in human neutrophils. This mechanism indicates a potentially new and important modulation factor for this fungal pathogen.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35322/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariseNunes_SuzanaCReal_etal_IOC_2019.pdfMariseNunes_SuzanaCReal_etal_IOC_2019.pdfapplication/pdf1108627https://www.arca.fiocruz.br/bitstream/icict/35322/2/MariseNunes_SuzanaCReal_etal_IOC_2019.pdfca75434d43f19d081589da7931f232b0MD52TEXTMariseNunes_SuzanaCReal_etal_IOC_2019.pdf.txtMariseNunes_SuzanaCReal_etal_IOC_2019.pdf.txtExtracted texttext/plain44607https://www.arca.fiocruz.br/bitstream/icict/35322/3/MariseNunes_SuzanaCReal_etal_IOC_2019.pdf.txt89cc28bec517e089ef8e69f41665ef1bMD53icict/353222022-06-24 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| dc.title.pt_BR.fl_str_mv |
Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils |
| title |
Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils |
| spellingShingle |
Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils Rocha, Juliana D. B. Polissacarídeos capsulares Cryptococcus neoformans Neutrófilos extracelulares armadilhas Neutrófilos humanos Capsular polysaccharides Cryptococcus neoformans Nneutrophil extracellular traps Human neutrophils |
| title_short |
Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils |
| title_full |
Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils |
| title_fullStr |
Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils |
| title_full_unstemmed |
Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils |
| title_sort |
Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils |
| author |
Rocha, Juliana D. B. |
| author_facet |
Rocha, Juliana D. B. Nascimento, Michelle T. C. Ricardo, Debora Decote Côrte-Real, Suzana Morrot, Alexandre Heise, Norton Nunes, Marise P. Previato, José Osvaldo Previato, Lucia Mendonça Reis, George A. dos Saraiva, Elvira M. Lima, Célio G. Freire de |
| author_role |
author |
| author2 |
Nascimento, Michelle T. C. Ricardo, Debora Decote Côrte-Real, Suzana Morrot, Alexandre Heise, Norton Nunes, Marise P. Previato, José Osvaldo Previato, Lucia Mendonça Reis, George A. dos Saraiva, Elvira M. Lima, Célio G. Freire de |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rocha, Juliana D. B. Nascimento, Michelle T. C. Ricardo, Debora Decote Côrte-Real, Suzana Morrot, Alexandre Heise, Norton Nunes, Marise P. Previato, José Osvaldo Previato, Lucia Mendonça Reis, George A. dos Saraiva, Elvira M. Lima, Célio G. Freire de |
| dc.subject.other.pt_BR.fl_str_mv |
Polissacarídeos capsulares Cryptococcus neoformans Neutrófilos extracelulares armadilhas Neutrófilos humanos |
| topic |
Polissacarídeos capsulares Cryptococcus neoformans Neutrófilos extracelulares armadilhas Neutrófilos humanos Capsular polysaccharides Cryptococcus neoformans Nneutrophil extracellular traps Human neutrophils |
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Capsular polysaccharides Cryptococcus neoformans Nneutrophil extracellular traps Human neutrophils |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de janeiro, RJ, Brasil. |
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2015 |
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2015 |
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2019-09-03T17:25:23Z |
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2019-09-03T17:25:23Z |
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ROCHA, Juliana D. B. et al. Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils. Scientific Reports, v. 5, n. 8008, p. 1-10, Jan. 2015. |
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https://www.arca.fiocruz.br/handle/icict/35322 |
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2045-2322 |
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10.1038/srep08008 |
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ROCHA, Juliana D. B. et al. Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils. Scientific Reports, v. 5, n. 8008, p. 1-10, Jan. 2015. 2045-2322 10.1038/srep08008 |
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