Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region

Bibliographic Details
Main Author: Tang, Thuy-Huong Ta
Publication Date: 2010
Other Authors: López-Vélez, Rogelio, Lanza, Marta, Shelley, Anthony John, Rubio, Jose Miguel, Luz, Sérgio Luiz Bessa
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/1811
Summary: Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España.
id CRUZ_a9b72eec1a01ebd1d861bd68b039de4a
oai_identifier_str oai:www.arca.fiocruz.br:icict/1811
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Tang, Thuy-Huong TaLópez-Vélez, RogelioLanza, MartaShelley, Anthony JohnRubio, Jose MiguelLuz, Sérgio Luiz Bessa2011-03-31T18:49:14Z2011-03-31T18:49:14Z2010TANG, Thuy-Huong Ta et al. Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 823-828, Sept. 2010.0074-0276https://www.arca.fiocruz.br/handle/icict/1811engNested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Regioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRio de JaneiroInstituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España.Hospital Ramón y Cajal. Unidad de Medicina Tropical y Parasitología Clínica. Servicio de Enfermedades Infecciosas. Madrid, España.Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España.Natural History Museum. Department of Entomology. London, UK.Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly sensitive and specific and it detects low-level infections in both humans and vectors. No cross-amplifications were observed with various other blood parasites and no false-positive results were obtained with the nested PCR. The method works efficiently with whole-blood, blood-spot and skin biopsy samples. Our method may thus be suitable for assessing the efficacy of filaria control programmes in Amazonia by recording parasite infections in both the human host and the vector. By specifically differentiating the major sympatric species of filaria, this technique could also enhance epidemiological research in the region.Nested PCR18S rDNAITS-1OnchocerciasisMansonelliasisBrazilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNested PCR to detect.pdfNested PCR to detect.pdfapplication/pdf557199https://www.arca.fiocruz.br/bitstream/icict/1811/1/Nested%20PCR%20to%20detect.pdf87e284a6bf219eb9ed2c8881cfa3d3adMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://www.arca.fiocruz.br/bitstream/icict/1811/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTNested PCR to detect.pdf.txtNested PCR to detect.pdf.txtExtracted texttext/plain32646https://www.arca.fiocruz.br/bitstream/icict/1811/5/Nested%20PCR%20to%20detect.pdf.txt74a93c2bcc1909001786aefbcff8cc6bMD55THUMBNAILNested PCR to detect.pdf.jpgNested PCR to detect.pdf.jpgGenerated Thumbnailimage/jpeg2416https://www.arca.fiocruz.br/bitstream/icict/1811/4/Nested%20PCR%20to%20detect.pdf.jpg11d35afec06622eb70fcff1222d93c21MD54icict/18112018-04-06 09:17:43.222oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T12:17:43Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
title Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
spellingShingle Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
Tang, Thuy-Huong Ta
Nested PCR
18S rDNA
ITS-1
Onchocerciasis
Mansonelliasis
Brazil
title_short Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
title_full Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
title_fullStr Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
title_full_unstemmed Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
title_sort Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
author Tang, Thuy-Huong Ta
author_facet Tang, Thuy-Huong Ta
López-Vélez, Rogelio
Lanza, Marta
Shelley, Anthony John
Rubio, Jose Miguel
Luz, Sérgio Luiz Bessa
author_role author
author2 López-Vélez, Rogelio
Lanza, Marta
Shelley, Anthony John
Rubio, Jose Miguel
Luz, Sérgio Luiz Bessa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Tang, Thuy-Huong Ta
López-Vélez, Rogelio
Lanza, Marta
Shelley, Anthony John
Rubio, Jose Miguel
Luz, Sérgio Luiz Bessa
dc.subject.en.pt_BR.fl_str_mv Nested PCR
18S rDNA
ITS-1
Onchocerciasis
Mansonelliasis
Brazil
topic Nested PCR
18S rDNA
ITS-1
Onchocerciasis
Mansonelliasis
Brazil
description Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2011-03-31T18:49:14Z
dc.date.available.fl_str_mv 2011-03-31T18:49:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TANG, Thuy-Huong Ta et al. Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 823-828, Sept. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/1811
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv TANG, Thuy-Huong Ta et al. Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 823-828, Sept. 2010.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/1811
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/1811/1/Nested%20PCR%20to%20detect.pdf
https://www.arca.fiocruz.br/bitstream/icict/1811/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/1811/5/Nested%20PCR%20to%20detect.pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/1811/4/Nested%20PCR%20to%20detect.pdf.jpg
bitstream.checksum.fl_str_mv 87e284a6bf219eb9ed2c8881cfa3d3ad
8a4605be74aa9ea9d79846c1fba20a33
74a93c2bcc1909001786aefbcff8cc6b
11d35afec06622eb70fcff1222d93c21
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798325056538935296

Similar Items