Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi

Bibliographic Details
Main Author: Sinha, Amit
Publication Date: 2023
Other Authors: Li, Zhiru, Poole, Catherine B., Morgan, Richard D., Ettwiller, Laurence, Lima, Nathália F., Ferreira, Marcelo U., Fombad, Fanny F., Wanji, Samuel, Carlow, Clotilde K.S.
Format: Article
Language: eng
Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Download full: http://hdl.handle.net/10362/164500
Summary: Funding Information: Research funding was provided by New England Biolabs, Ipswich, MA 01938, USA to AS, ZL, LE, RM, CP, CC. FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) supported the field research in Brazil through a research grant to M.U.F (2013/12723-7) and a doctoral scholarship to NL (2013/26928-0). Acknowledgments Funding Information: Research funding was provided by New England Biolabs, Ipswich, MA 01938, USA to AS, ZL, LE, RM, CP, CC. FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) supported the field research in Brazil through a research grant to M.U.F (2013/12723-7) and a doctoral scholarship to NL (2013/26928-0). Publisher Copyright: Copyright © 2023 Sinha, Li, Poole, Morgan, Ettwiller, Lima, Ferreira, Fombad, Wanji and Carlow.
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spelling Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardievolutionfilariagenomicsMansonellaneglected tropical diseaseQH426 GeneticsQR MicrobiologyInfectious DiseasesMicrobiology (medical)Public Health, Environmental and Occupational HealthParasitologySDG 3 - Good Health and Well-beingFunding Information: Research funding was provided by New England Biolabs, Ipswich, MA 01938, USA to AS, ZL, LE, RM, CP, CC. FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) supported the field research in Brazil through a research grant to M.U.F (2013/12723-7) and a doctoral scholarship to NL (2013/26928-0). Acknowledgments Funding Information: Research funding was provided by New England Biolabs, Ipswich, MA 01938, USA to AS, ZL, LE, RM, CP, CC. FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) supported the field research in Brazil through a research grant to M.U.F (2013/12723-7) and a doctoral scholarship to NL (2013/26928-0). Publisher Copyright: Copyright © 2023 Sinha, Li, Poole, Morgan, Ettwiller, Lima, Ferreira, Fombad, Wanji and Carlow.The filarial parasites Mansonella ozzardi and Mansonella perstans, causative agents of mansonellosis, infect hundreds of millions of people worldwide, yet remain among the most understudied of the human filarial pathogens. M. ozzardi is highly prevalent in Latin American countries and Caribbean Islands, while M. perstans is predominantly found in sub-Saharan Africa as well as in a few areas in South America. In addition to the differences in their geographical distribution, the two parasites are transmitted by different insect vectors, as well as exhibit differences in their responses to commonly used anthelminthic drugs. The lack of genome information has hindered investigations into the biology and evolution of Mansonella parasites and understanding the molecular basis of the clinical differences between species. In the current study, high quality genomes of two independent clinical isolates of M. perstans from Cameroon and two M. ozzardi isolates one from Brazil and one from Venezuela are reported. The genomes are approximately 76 Mb in size, encode about 10,000 genes each, and are largely complete based on BUSCO scores of about 90%, similar to other completed filarial genomes. These sequences represent the first genomes from Mansonella parasites and enabled a comparative genomic analysis of the similarities and differences between Mansonella and other filarial parasites. Horizontal DNA transfers (HDT) from mitochondria (nuMTs) as well as transfers from genomes of endosymbiotic Wolbachia bacteria (nuWTs) to the host nuclear genome were identified and analyzed. Sequence comparisons and phylogenetic analysis of known targets of anti-filarial drugs diethylcarbamazine (DEC), ivermectin and mebendazole revealed that all known target genes were present in both species, except for the DEC target encoded by gon-2 gene, which is fragmented in genome assemblies from both M. ozzardi isolates. These new reference genome sequences will provide a valuable resource for further studies on biology, symbiosis, evolution and drug discovery.Individual Health Care (IHC)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNSinha, AmitLi, ZhiruPoole, Catherine B.Morgan, Richard D.Ettwiller, LaurenceLima, Nathália F.Ferreira, Marcelo U.Fombad, Fanny F.Wanji, SamuelCarlow, Clotilde K.S.2024-03-05T23:49:44Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/164500engPURE: 83836939https://doi.org/10.3389/fitd.2023.1139343info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:52:41Zoai:run.unl.pt:10362/164500Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:12.750326Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi
title Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi
spellingShingle Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi
Sinha, Amit
evolution
filaria
genomics
Mansonella
neglected tropical disease
QH426 Genetics
QR Microbiology
Infectious Diseases
Microbiology (medical)
Public Health, Environmental and Occupational Health
Parasitology
SDG 3 - Good Health and Well-being
title_short Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi
title_full Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi
title_fullStr Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi
title_full_unstemmed Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi
title_sort Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi
author Sinha, Amit
author_facet Sinha, Amit
Li, Zhiru
Poole, Catherine B.
Morgan, Richard D.
Ettwiller, Laurence
Lima, Nathália F.
Ferreira, Marcelo U.
Fombad, Fanny F.
Wanji, Samuel
Carlow, Clotilde K.S.
author_role author
author2 Li, Zhiru
Poole, Catherine B.
Morgan, Richard D.
Ettwiller, Laurence
Lima, Nathália F.
Ferreira, Marcelo U.
Fombad, Fanny F.
Wanji, Samuel
Carlow, Clotilde K.S.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Individual Health Care (IHC)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Sinha, Amit
Li, Zhiru
Poole, Catherine B.
Morgan, Richard D.
Ettwiller, Laurence
Lima, Nathália F.
Ferreira, Marcelo U.
Fombad, Fanny F.
Wanji, Samuel
Carlow, Clotilde K.S.
dc.subject.por.fl_str_mv evolution
filaria
genomics
Mansonella
neglected tropical disease
QH426 Genetics
QR Microbiology
Infectious Diseases
Microbiology (medical)
Public Health, Environmental and Occupational Health
Parasitology
SDG 3 - Good Health and Well-being
topic evolution
filaria
genomics
Mansonella
neglected tropical disease
QH426 Genetics
QR Microbiology
Infectious Diseases
Microbiology (medical)
Public Health, Environmental and Occupational Health
Parasitology
SDG 3 - Good Health and Well-being
description Funding Information: Research funding was provided by New England Biolabs, Ipswich, MA 01938, USA to AS, ZL, LE, RM, CP, CC. FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) supported the field research in Brazil through a research grant to M.U.F (2013/12723-7) and a doctoral scholarship to NL (2013/26928-0). Acknowledgments Funding Information: Research funding was provided by New England Biolabs, Ipswich, MA 01938, USA to AS, ZL, LE, RM, CP, CC. FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) supported the field research in Brazil through a research grant to M.U.F (2013/12723-7) and a doctoral scholarship to NL (2013/26928-0). Publisher Copyright: Copyright © 2023 Sinha, Li, Poole, Morgan, Ettwiller, Lima, Ferreira, Fombad, Wanji and Carlow.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01T00:00:00Z
2024-03-05T23:49:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/164500
url http://hdl.handle.net/10362/164500
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 83836939
https://doi.org/10.3389/fitd.2023.1139343
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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