Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.

Detalhes bibliográficos
Autor(a) principal: Toledo, Juliano Simões de
Data de Publicação: 2009
Outros Autores: Santos, André Filipe Junqueira dos, Moura, Tatiana Rodrigues de, Antoniazi, Simone Aparecida, Brodskyn, Claudia Ida, Oliveira, Camila Indiani de, Barral, Aldina Maria Prado, Cruz, Angela Kaysel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8387
Resumo: Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil
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spelling Toledo, Juliano Simões deSantos, André Filipe Junqueira dosMoura, Tatiana Rodrigues deAntoniazi, Simone AparecidaBrodskyn, Claudia IdaOliveira, Camila Indiani deBarral, Aldina Maria PradoCruz, Angela Kaysel2014-09-16T14:23:08Z2014-09-16T14:23:08Z2009TOLEDO, J. S. et al. Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. Parasitology International, v. 58, n. 1, p. 45-50, 2009.1383-5769https://www.arca.fiocruz.br/handle/icict/838710.1016/j.parint.2008.09.006engElsevier LtdLeishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, BrasilThe miniexon gene has a central role in the processing of polycistronic pre-mRNA of kinetoplastids. It is added to the 5′ extremity of each mRNA, supplying the 5′-capped structure to the molecule. Previous studies in Leishmania (Leishmania) major showed that the overexpression of the miniexon array attenuates the virulence of the parasite in in vivo assays. The results presented here extend those findings to Viannia subgenus. Leishmania (Viannia) braziliensis was transfected with a cosmid harboring a tandem array of one hundred miniexon gene copies and then characterized by Northern blot analysis. The overexpression of the exogenous gene was confirmed and its effect on the virulence of L. (V.) braziliensis was investigated in hamsters. In BALB/c mice we could not detect parasites during the course of 15 weeks of infection. In addition, hamsters infected with transfectants overexpressing the miniexon gene exhibited only a minor footpad swelling of late onset and failed to develop progressive lesion, these attenuated parasites could be recovered from the inoculation site 1 year after infection. The persistence of parasites in the host indicates that a stable line overexpressing the miniexon may be tested as live vaccine against leishmaniasis.Leishmania (V.) braziliensisVirulenceMiniexonOverexpressionSL RNAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8387/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALToledo JS Leishmania (Viannia) braziliensis.........pdfToledo JS Leishmania (Viannia) braziliensis.........pdfapplication/pdf842067https://www.arca.fiocruz.br/bitstream/icict/8387/2/Toledo%20JS%20Leishmania%20%28Viannia%29%20braziliensis.........pdfde2d7720e98de4153164e594df75b5b1MD52TEXTToledo JS Leishmania (Viannia) braziliensis.........pdf.txtToledo JS Leishmania (Viannia) braziliensis.........pdf.txtExtracted texttext/plain31699https://www.arca.fiocruz.br/bitstream/icict/8387/3/Toledo%20JS%20Leishmania%20%28Viannia%29%20braziliensis.........pdf.txt1dc5e9593f9709a89515cb4c19d26149MD53icict/83872023-03-15 14:33:18.603oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
title Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
spellingShingle Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
Toledo, Juliano Simões de
Leishmania (V.) braziliensis
Virulence
Miniexon
Overexpression
SL RNA
title_short Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
title_full Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
title_fullStr Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
title_full_unstemmed Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
title_sort Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
author Toledo, Juliano Simões de
author_facet Toledo, Juliano Simões de
Santos, André Filipe Junqueira dos
Moura, Tatiana Rodrigues de
Antoniazi, Simone Aparecida
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Cruz, Angela Kaysel
author_role author
author2 Santos, André Filipe Junqueira dos
Moura, Tatiana Rodrigues de
Antoniazi, Simone Aparecida
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Cruz, Angela Kaysel
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Toledo, Juliano Simões de
Santos, André Filipe Junqueira dos
Moura, Tatiana Rodrigues de
Antoniazi, Simone Aparecida
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Cruz, Angela Kaysel
dc.subject.en.pt_BR.fl_str_mv Leishmania (V.) braziliensis
Virulence
Miniexon
Overexpression
SL RNA
topic Leishmania (V.) braziliensis
Virulence
Miniexon
Overexpression
SL RNA
description Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2014-09-16T14:23:08Z
dc.date.available.fl_str_mv 2014-09-16T14:23:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TOLEDO, J. S. et al. Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. Parasitology International, v. 58, n. 1, p. 45-50, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8387
dc.identifier.issn.none.fl_str_mv 1383-5769
dc.identifier.doi.none.fl_str_mv 10.1016/j.parint.2008.09.006
identifier_str_mv TOLEDO, J. S. et al. Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. Parasitology International, v. 58, n. 1, p. 45-50, 2009.
1383-5769
10.1016/j.parint.2008.09.006
url https://www.arca.fiocruz.br/handle/icict/8387
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier Ltd
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