Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8387 |
Resumo: | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil |
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Toledo, Juliano Simões deSantos, André Filipe Junqueira dosMoura, Tatiana Rodrigues deAntoniazi, Simone AparecidaBrodskyn, Claudia IdaOliveira, Camila Indiani deBarral, Aldina Maria PradoCruz, Angela Kaysel2014-09-16T14:23:08Z2014-09-16T14:23:08Z2009TOLEDO, J. S. et al. Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. Parasitology International, v. 58, n. 1, p. 45-50, 2009.1383-5769https://www.arca.fiocruz.br/handle/icict/838710.1016/j.parint.2008.09.006engElsevier LtdLeishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, BrasilThe miniexon gene has a central role in the processing of polycistronic pre-mRNA of kinetoplastids. It is added to the 5′ extremity of each mRNA, supplying the 5′-capped structure to the molecule. Previous studies in Leishmania (Leishmania) major showed that the overexpression of the miniexon array attenuates the virulence of the parasite in in vivo assays. The results presented here extend those findings to Viannia subgenus. Leishmania (Viannia) braziliensis was transfected with a cosmid harboring a tandem array of one hundred miniexon gene copies and then characterized by Northern blot analysis. The overexpression of the exogenous gene was confirmed and its effect on the virulence of L. (V.) braziliensis was investigated in hamsters. In BALB/c mice we could not detect parasites during the course of 15 weeks of infection. In addition, hamsters infected with transfectants overexpressing the miniexon gene exhibited only a minor footpad swelling of late onset and failed to develop progressive lesion, these attenuated parasites could be recovered from the inoculation site 1 year after infection. The persistence of parasites in the host indicates that a stable line overexpressing the miniexon may be tested as live vaccine against leishmaniasis.Leishmania (V.) braziliensisVirulenceMiniexonOverexpressionSL RNAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8387/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALToledo JS Leishmania (Viannia) braziliensis.........pdfToledo JS Leishmania (Viannia) braziliensis.........pdfapplication/pdf842067https://www.arca.fiocruz.br/bitstream/icict/8387/2/Toledo%20JS%20Leishmania%20%28Viannia%29%20braziliensis.........pdfde2d7720e98de4153164e594df75b5b1MD52TEXTToledo JS Leishmania (Viannia) braziliensis.........pdf.txtToledo JS Leishmania (Viannia) braziliensis.........pdf.txtExtracted texttext/plain31699https://www.arca.fiocruz.br/bitstream/icict/8387/3/Toledo%20JS%20Leishmania%20%28Viannia%29%20braziliensis.........pdf.txt1dc5e9593f9709a89515cb4c19d26149MD53icict/83872023-03-15 14:33:18.603oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. |
title |
Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. |
spellingShingle |
Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. Toledo, Juliano Simões de Leishmania (V.) braziliensis Virulence Miniexon Overexpression SL RNA |
title_short |
Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. |
title_full |
Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. |
title_fullStr |
Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. |
title_full_unstemmed |
Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. |
title_sort |
Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. |
author |
Toledo, Juliano Simões de |
author_facet |
Toledo, Juliano Simões de Santos, André Filipe Junqueira dos Moura, Tatiana Rodrigues de Antoniazi, Simone Aparecida Brodskyn, Claudia Ida Oliveira, Camila Indiani de Barral, Aldina Maria Prado Cruz, Angela Kaysel |
author_role |
author |
author2 |
Santos, André Filipe Junqueira dos Moura, Tatiana Rodrigues de Antoniazi, Simone Aparecida Brodskyn, Claudia Ida Oliveira, Camila Indiani de Barral, Aldina Maria Prado Cruz, Angela Kaysel |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Toledo, Juliano Simões de Santos, André Filipe Junqueira dos Moura, Tatiana Rodrigues de Antoniazi, Simone Aparecida Brodskyn, Claudia Ida Oliveira, Camila Indiani de Barral, Aldina Maria Prado Cruz, Angela Kaysel |
dc.subject.en.pt_BR.fl_str_mv |
Leishmania (V.) braziliensis Virulence Miniexon Overexpression SL RNA |
topic |
Leishmania (V.) braziliensis Virulence Miniexon Overexpression SL RNA |
description |
Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2014-09-16T14:23:08Z |
dc.date.available.fl_str_mv |
2014-09-16T14:23:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TOLEDO, J. S. et al. Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. Parasitology International, v. 58, n. 1, p. 45-50, 2009. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8387 |
dc.identifier.issn.none.fl_str_mv |
1383-5769 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.parint.2008.09.006 |
identifier_str_mv |
TOLEDO, J. S. et al. Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. Parasitology International, v. 58, n. 1, p. 45-50, 2009. 1383-5769 10.1016/j.parint.2008.09.006 |
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https://www.arca.fiocruz.br/handle/icict/8387 |
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eng |
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Elsevier Ltd |
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Elsevier Ltd |
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