Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.

Detalhes bibliográficos
Autor(a) principal: Paes, R. A.
Data de Publicação: 2007
Outros Autores: Pimenta, M. A., Monteiro, P. C. F., Nosanchuk, J. D., Zancopé-Oliveira, R. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33253
Resumo: 2019-11-28
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spelling Paes, R. A.Pimenta, M. A.Monteiro, P. C. F.Nosanchuk, J. D.Zancopé-Oliveira, R. M.2019-05-28T14:56:38Z2019-05-28T14:56:38Z2007ALMEIDA-PAES Rodrigo de et al. Immunoglobulins G, M, and A against Sporothrix schenckii Exoantigens in Patients with Sporotrichosis before and during Treatment with Itraconazole. Clinical Vaccine Immunology, v. 14, p. 1149-1157, 2007.1556-6811https://www.arca.fiocruz.br/handle/icict/3325310.1128/CVI.00149-071556-679XengAmerican Society for MicrobiologyImmunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2019-11-28Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil / Albert Einstein College of Medicine. Department of Medicine. Division of Infectious Diseases. New York, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, BrasilAlbert Einstein College of Medicine. Department of Medicine. Division of Infectious Diseases. New York, NY, USA / Albert Einstein College of Medicine. Department of Microbiology and Immunology. New York, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.Sporotrichosis is an important subcutaneous mycosis, with an increasing worldwide incidence. However, few data are available regarding the immunological aspects of Sporothrix schenckii infection, particularly the humoral responses to the fungus. In this study we measured immunoglobulin G (IgG), IgM, and IgA in sera from 41 patients with sporotrichosis before antifungal treatment and from another 35 patients with sporotrichosis during itraconazole treatment by using a recently described S. schenckii exoantigen enzyme-linked immunosorbent assay (ELISA). More than 95% of patients had detectable IgA antibodies, and more than 85% had IgM and IgG antibodies before treatment. The number of patients with IgG antibodies increased to 91% during treatment. Conversely, significantly fewer samples from treated patients were positive for IgM (71%) and IgA (89%). Overall, 78% of patients had detectable levels of all isotypes tested at diagnosis, and this percentage dropped to 62.9% in patients receiving itraconazole. Testing of all three isotypes improved the sensitivity; at least two isotypes were detected in 93% of patients before and 89% after treatment. The reactivity of 94 sera from patients with other diseases and healthy individuals was also tested. Cross-reactivity occurred in 33% of the heterologous sera. Most of them were positive only in one isotype, 8.5% were positive for at least two isotypes, and only one serum (1.1%) was positive for the three isotypes. Antibodies produced during S. schenckii infection are diverse, and we demonstrate that an exoantigen ELISA for the detection of combinations of IgA, IgG, and IgM antibodies is a highly sensitive and specific diagnostic assay for sporotrichosis.SporotrichosisSporothrix schenckiiImmunoglobulin GImmunoglobulin MImmunoglobulin AItraconazoleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33253/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Almeida-Paes_Rodrigo_etal_INI_2007.pdfve_Almeida-Paes_Rodrigo_etal_INI_2007.pdfapplication/pdf310047https://www.arca.fiocruz.br/bitstream/icict/33253/2/ve_Almeida-Paes_Rodrigo_etal_INI_2007.pdf6e6b3c76a5f9432449d1b25254e65aa6MD52TEXTve_Almeida-Paes_Rodrigo_etal_INI_2007.pdf.txtve_Almeida-Paes_Rodrigo_etal_INI_2007.pdf.txtExtracted texttext/plain46436https://www.arca.fiocruz.br/bitstream/icict/33253/3/ve_Almeida-Paes_Rodrigo_etal_INI_2007.pdf.txt61a5ed92122a74ec594f7838759a5a42MD53icict/332532021-03-24 16:32:25.392oai:www.arca.fiocruz.br:icict/33253Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
title Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
spellingShingle Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
Paes, R. A.
Sporotrichosis
Sporothrix schenckii
Immunoglobulin G
Immunoglobulin M
Immunoglobulin A
Itraconazole
title_short Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
title_full Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
title_fullStr Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
title_full_unstemmed Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
title_sort Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
author Paes, R. A.
author_facet Paes, R. A.
Pimenta, M. A.
Monteiro, P. C. F.
Nosanchuk, J. D.
Zancopé-Oliveira, R. M.
author_role author
author2 Pimenta, M. A.
Monteiro, P. C. F.
Nosanchuk, J. D.
Zancopé-Oliveira, R. M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Paes, R. A.
Pimenta, M. A.
Monteiro, P. C. F.
Nosanchuk, J. D.
Zancopé-Oliveira, R. M.
dc.subject.en.pt_BR.fl_str_mv Sporotrichosis
Sporothrix schenckii
Immunoglobulin G
Immunoglobulin M
Immunoglobulin A
Itraconazole
topic Sporotrichosis
Sporothrix schenckii
Immunoglobulin G
Immunoglobulin M
Immunoglobulin A
Itraconazole
description 2019-11-28
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2019-05-28T14:56:38Z
dc.date.available.fl_str_mv 2019-05-28T14:56:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA-PAES Rodrigo de et al. Immunoglobulins G, M, and A against Sporothrix schenckii Exoantigens in Patients with Sporotrichosis before and during Treatment with Itraconazole. Clinical Vaccine Immunology, v. 14, p. 1149-1157, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33253
dc.identifier.issn.pt_BR.fl_str_mv 1556-6811
dc.identifier.doi.none.fl_str_mv 10.1128/CVI.00149-07
dc.identifier.eissn.none.fl_str_mv 1556-679X
identifier_str_mv ALMEIDA-PAES Rodrigo de et al. Immunoglobulins G, M, and A against Sporothrix schenckii Exoantigens in Patients with Sporotrichosis before and during Treatment with Itraconazole. Clinical Vaccine Immunology, v. 14, p. 1149-1157, 2007.
1556-6811
10.1128/CVI.00149-07
1556-679X
url https://www.arca.fiocruz.br/handle/icict/33253
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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