Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii

Detalhes bibliográficos
Autor(a) principal: Severo, Vanessa
Data de Publicação: 2023
Outros Autores: Souza, Rodolpho, Vitorino, Francisca, Cunha, Julia, Ávila, Andréa, Arrizabalaga, Gustavo, Nardelli, Sheila
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57005
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil.
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spelling Severo, VanessaSouza, RodolphoVitorino, FranciscaCunha, JuliaÁvila, AndréaArrizabalaga, GustavoNardelli, Sheila2023-02-10T16:06:24Z2023-02-10T16:06:24Z2023SEVERO, Vanessa et al. Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii. mSphere., v. 7 n. 6, p. 1–19, 2022.2379-5042https://www.arca.fiocruz.br/handle/icict/5700510.1128/msphere.00403-22porAmerican Society for MicrobiologyToxoplasma gondiiModificação Pós-TraducionalChromatinHistone H1Cell DivisionProtein Processing, Post-TranslationalCromatinaDivisión CelularProcesamiento Proteico-PostraduccionalChromatineHistoneDivision cellulaireMaturation post-traductionnelle des protéinesCromatinaHistonasDivisão CelularProcessamento de Proteína Pós-TraducionalPreviously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil.Department of Pharmacology and Toxicology. Indiana University School of Medicine. Indianapolis, Indiana, USA.Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil.Department of Pharmacology and Toxicology. Indiana University School of Medicine. Indianapolis, Indiana, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil.Chromatin dynamics can regulate all DNA-dependent processes. Access to DNA within chromatin is orchestrated mainly by histones and their posttranslational modifications (PTMs). Like other eukaryotes, the apicomplexan parasite Toxoplasma gondii encodes four canonical histones and five histone variants. In contrast, the linker histone (H1) has never been identified in apicomplexan parasites. In other eukaryotes, histone H1 compacts the chromatin by linking the nucleosome and increasing the DNA compaction. H1 is a multifunctional protein and can be involved in different steps of DNA metabolism or associated with protein complexes related to distinct biological processes. We have identified a novel protein in T. gondii (“TgH1-like”) that, although lacking the globular domain of mammalian H1, is remarkably like the H1-like proteins of bacteria and trypanosomatids. Our results demonstrate that TgH1-like is a nuclear protein associated with chromatin and other histones. Curiously, TgH1-like is also in the nucleolus and associated with ribosomal proteins, indicating a versatile function in this parasite. Although knockout of the tgh1-like gene does not affect the cell cycle, it causes endopolygeny and asynchronous division. Interestingly, mutation of posttranslationally modified amino acids results in defects in cell division like those in the Dtgh1-like mutant, showing that these sites are important for protein function. Furthermore, in the bradyzoite stage, this protein is expressed only in dividing parasites, reinforcing its importance in cell division. Indeed, the absence of TgH1-like decreases compaction of peripheral chromatin, confirming its role in the chromatin modulation in T. gondii.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57005/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmsphere.00403-22ok.pdfmsphere.00403-22ok.pdfapplication/pdf6223551https://www.arca.fiocruz.br/bitstream/icict/57005/2/msphere.00403-22ok.pdfb17681bdea48fa51b4f2ce7c1e48758bMD52icict/570052023-02-10 13:06:25.693oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-02-10T16:06:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
title Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
spellingShingle Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
Severo, Vanessa
Toxoplasma gondii
Modificação Pós-Traducional
Chromatin
Histone H1
Cell Division
Protein Processing, Post-Translational
Cromatina
División Celular
Procesamiento Proteico-Postraduccional
Chromatine
Histone
Division cellulaire
Maturation post-traductionnelle des protéines
Cromatina
Histonas
Divisão Celular
Processamento de Proteína Pós-Traducional
title_short Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
title_full Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
title_fullStr Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
title_full_unstemmed Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
title_sort Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
author Severo, Vanessa
author_facet Severo, Vanessa
Souza, Rodolpho
Vitorino, Francisca
Cunha, Julia
Ávila, Andréa
Arrizabalaga, Gustavo
Nardelli, Sheila
author_role author
author2 Souza, Rodolpho
Vitorino, Francisca
Cunha, Julia
Ávila, Andréa
Arrizabalaga, Gustavo
Nardelli, Sheila
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Severo, Vanessa
Souza, Rodolpho
Vitorino, Francisca
Cunha, Julia
Ávila, Andréa
Arrizabalaga, Gustavo
Nardelli, Sheila
dc.subject.other.en_US.fl_str_mv Toxoplasma gondii
Modificação Pós-Traducional
topic Toxoplasma gondii
Modificação Pós-Traducional
Chromatin
Histone H1
Cell Division
Protein Processing, Post-Translational
Cromatina
División Celular
Procesamiento Proteico-Postraduccional
Chromatine
Histone
Division cellulaire
Maturation post-traductionnelle des protéines
Cromatina
Histonas
Divisão Celular
Processamento de Proteína Pós-Traducional
dc.subject.en.en_US.fl_str_mv Chromatin
Histone H1
Cell Division
Protein Processing, Post-Translational
dc.subject.es.en_US.fl_str_mv Cromatina
División Celular
Procesamiento Proteico-Postraduccional
dc.subject.fr.en_US.fl_str_mv Chromatine
Histone
Division cellulaire
Maturation post-traductionnelle des protéines
dc.subject.decs.en_US.fl_str_mv Cromatina
Histonas
Divisão Celular
Processamento de Proteína Pós-Traducional
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-02-10T16:06:24Z
dc.date.available.fl_str_mv 2023-02-10T16:06:24Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SEVERO, Vanessa et al. Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii. mSphere., v. 7 n. 6, p. 1–19, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57005
dc.identifier.issn.en_US.fl_str_mv 2379-5042
dc.identifier.doi.none.fl_str_mv 10.1128/msphere.00403-22
identifier_str_mv SEVERO, Vanessa et al. Previously unidentified histone H1-like protein is involved in cell division and ribosome biosynthesis in Toxoplasma gondii. mSphere., v. 7 n. 6, p. 1–19, 2022.
2379-5042
10.1128/msphere.00403-22
url https://www.arca.fiocruz.br/handle/icict/57005
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57005/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/57005/2/msphere.00403-22ok.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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