CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7199 |
Resumo: | Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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Santos, Claire da SilvaOliveira, Viviane Sampaio Boaventura deCardoso, Cristina Ribeiro de BarrosTavares, Natalia MachadoLordelo, Morgana JNoronha, Almério Libório Lopes deCosta, Jackson Mauricio LopesBorges, Valeria de MatosOliveira, Camila Indiani deVan Weyenbergh, Johan Jozef Rosa MariaBarral, Aldina Maria PradoBarral Netto, ManoelBrodskyn, Claudia Ida2013-10-24T14:12:11Z2013-10-24T14:12:11Z2013SANTOS, C. da S. et al. CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis. Journal of Investigative Dermatology, v. 133, n. 6, p. 1533-1540, 2013.1523-1747https://www.arca.fiocruz.br/handle/icict/7199engThe Society for Investigative DermatologyCD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas . São Paulo, SP, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilA protective or deleterious role of CD8þT cells in human cutaneous leishmaniasis (CL) has been debated. The present report explores the participation of CD8þT cells in disease pathogenesis as well as in parasite killing. CD8þT cells accumulated in CL lesions as suggested by a higher frequency of CD8þCD45ROþT cells and CD8þCLAþT cells compared with peripheral blood mononuclear cells. Upon Leishmania braziliensis restimulation, most of the CD8þT cells from the lesion expressed cytolytic markers, CD107a and granzyme B. Granzyme B expression in CL lesions positively correlated with lesion size and percentage of TUNEL-positive cells. We also observed a significantly higher percentage of TUNEL-positive cells and granzyme B expression in the biopsies of patients showing a more intense necrotic process. Furthermore, coculture of infected macrophages and CD8þ T lymphocytes resulted in the release of granzyme B, and the use of granzyme B inhibitor, as well as z-VAD, Fas:Fc, or anti-IFN-g, had no effect upon parasite killing. However, coculture of infected macrophages with CD4þT cells strongly increased parasite killing, which was completely reversed by anti-IFN-g. Our results reveal a dichotomy in human CL: CD8þ granzyme BþT cells mediate tissue injury, whereas CD4þIFN-gþT cells mediate parasite killing.Linfócitos T CD4-Positivos/imunologiaLinfócitos T CD8-Positivos/imunologiaGranzimas/imunologiaInterferon gama/imunologiaLeishmania braziliensis/imunologiaLeishmaniose Cutânea/imunologiaAdolescenteAdultoMarcadores Biológicos/metabolismoMovimento Celular/imunologiaCélulas CultivadasTécnicas de CoculturaMarcação In Situ das Extremidades CortadasLeishmaniose Cutânea/patologiaPele/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALClaire SS CD8+ granzyme b...pdfClaire SS CD8+ granzyme b...pdfapplication/pdf4914876https://www.arca.fiocruz.br/bitstream/icict/7199/1/Claire%20SS%20CD8%2b%20granzyme%20b...pdfe54c8737a502be3105cd578fa7d10ef4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81867https://www.arca.fiocruz.br/bitstream/icict/7199/2/license.txt89d048e714e969a1adcda05fdb1978beMD52TEXTClaire SS CD8+ granzyme b...pdf.txtClaire SS CD8+ granzyme b...pdf.txtExtracted texttext/plain43172https://www.arca.fiocruz.br/bitstream/icict/7199/5/Claire%20SS%20CD8%2b%20granzyme%20b...pdf.txt2a17b9acb4357b286b0c002c3099f6e9MD55THUMBNAILClaire SS CD8+ granzyme b...pdf.jpgClaire SS CD8+ granzyme b...pdf.jpgGenerated Thumbnailimage/jpeg2401https://www.arca.fiocruz.br/bitstream/icict/7199/4/Claire%20SS%20CD8%2b%20granzyme%20b...pdf.jpg7b560361d263dfc6eff739f1c1008df8MD54icict/71992023-03-15 14:32:54.542oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis |
| title |
CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis |
| spellingShingle |
CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis Santos, Claire da Silva Linfócitos T CD4-Positivos/imunologia Linfócitos T CD8-Positivos/imunologia Granzimas/imunologia Interferon gama/imunologia Leishmania braziliensis/imunologia Leishmaniose Cutânea/imunologia Adolescente Adulto Marcadores Biológicos/metabolismo Movimento Celular/imunologia Células Cultivadas Técnicas de Cocultura Marcação In Situ das Extremidades Cortadas Leishmaniose Cutânea/patologia Pele/imunologia |
| title_short |
CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis |
| title_full |
CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis |
| title_fullStr |
CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis |
| title_full_unstemmed |
CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis |
| title_sort |
CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis |
| author |
Santos, Claire da Silva |
| author_facet |
Santos, Claire da Silva Oliveira, Viviane Sampaio Boaventura de Cardoso, Cristina Ribeiro de Barros Tavares, Natalia Machado Lordelo, Morgana J Noronha, Almério Libório Lopes de Costa, Jackson Mauricio Lopes Borges, Valeria de Matos Oliveira, Camila Indiani de Van Weyenbergh, Johan Jozef Rosa Maria Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
| author_role |
author |
| author2 |
Oliveira, Viviane Sampaio Boaventura de Cardoso, Cristina Ribeiro de Barros Tavares, Natalia Machado Lordelo, Morgana J Noronha, Almério Libório Lopes de Costa, Jackson Mauricio Lopes Borges, Valeria de Matos Oliveira, Camila Indiani de Van Weyenbergh, Johan Jozef Rosa Maria Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Claire da Silva Oliveira, Viviane Sampaio Boaventura de Cardoso, Cristina Ribeiro de Barros Tavares, Natalia Machado Lordelo, Morgana J Noronha, Almério Libório Lopes de Costa, Jackson Mauricio Lopes Borges, Valeria de Matos Oliveira, Camila Indiani de Van Weyenbergh, Johan Jozef Rosa Maria Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
| dc.subject.decs.pt_BR.fl_str_mv |
Linfócitos T CD4-Positivos/imunologia Linfócitos T CD8-Positivos/imunologia Granzimas/imunologia Interferon gama/imunologia Leishmania braziliensis/imunologia Leishmaniose Cutânea/imunologia Adolescente Adulto Marcadores Biológicos/metabolismo Movimento Celular/imunologia Células Cultivadas Técnicas de Cocultura Marcação In Situ das Extremidades Cortadas Leishmaniose Cutânea/patologia Pele/imunologia |
| topic |
Linfócitos T CD4-Positivos/imunologia Linfócitos T CD8-Positivos/imunologia Granzimas/imunologia Interferon gama/imunologia Leishmania braziliensis/imunologia Leishmaniose Cutânea/imunologia Adolescente Adulto Marcadores Biológicos/metabolismo Movimento Celular/imunologia Células Cultivadas Técnicas de Cocultura Marcação In Situ das Extremidades Cortadas Leishmaniose Cutânea/patologia Pele/imunologia |
| description |
Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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2013 |
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2013-10-24T14:12:11Z |
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2013-10-24T14:12:11Z |
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2013 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SANTOS, C. da S. et al. CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis. Journal of Investigative Dermatology, v. 133, n. 6, p. 1533-1540, 2013. |
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https://www.arca.fiocruz.br/handle/icict/7199 |
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1523-1747 |
| identifier_str_mv |
SANTOS, C. da S. et al. CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis. Journal of Investigative Dermatology, v. 133, n. 6, p. 1533-1540, 2013. 1523-1747 |
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eng |
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The Society for Investigative Dermatology |
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The Society for Investigative Dermatology |
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