pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/21004 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil |
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da Silva, Luís Cláudio NascimentoAlves, Neyla Maria Pereirade Castro, Maria Carolina Accioly BrelazHigino, Taciana Mirely Macielda Cunha, Cássia Regina AlbuquerquePereira, Valéria Rêgo Alvesda Paz, Nathalia Varejão NogueiraCoelho, Luana Cassandra Breitenbach BarrosoCorreia, Maria Tereza dos Santosde Figueiredo, Regina Celia Bressan Queiroz2017-09-13T18:45:46Z2017-09-13T18:45:46Z2015DA SILVA, L. C. N. et al. pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide. Oxidative Medicine and Cellular Longevity, v. 2015, p. 520872, 2015.1942-0994https://www.arca.fiocruz.br/handle/icict/2100410.1155/2015/520872porPeróxido de HidrogênioLectinasEstresse OxidativoSubstâncias ProtetorasAnimalsApoptosisCell ProliferationCercopithecus aethiopsDNA DamageFabaceaeHydrogen PeroxideLectinsMitochondria/Oxidative StressProtective AgentsReactive Oxygen SpeciesRecombinant ProteinsSeedsVero CellsPeróxido de HidrogênioLectinasEstresse OxidativoSubstâncias ProtetorasAnimaisProliferação CelularApoptoseCercopithecus aethiopsDano ao DNAFabaceaePotencial da Membrana MitocondrialMitocôndriasEspécies Reativas de OxigênioProteínas RecombinantesSementesCélulas VeropCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxideinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilOxidative stress plays an important role in the induction of cell death and is associated with various pathologic disorders; therefore, the search for natural products that attenuate the effects produced by oxidant agents is greatly increased. Here, the protective effects of native lectin from Cratylia mollis seeds (pCramoll) and recombinant Cramoll 1 (rCramoll) against H2O2-induced oxidative stress in Vero cells were evaluated. Both lectins significantly attenuated the H2O2-induced cytotoxicity in a concentration-dependent way. The maximum protective effects were 96.85 ± 15.59% (rCramoll) and 59.48 ± 23.44% (pCramoll). The Live/Dead analysis showed a reduction in the percentage of dead cells from 65.04 ± 3.29% (H2O2) to 39.77 ± 2.93% (pCramoll) and 13.90 ± 9.01% (rCramoll). The deleterious effects of H2O2 on cell proliferation were reduced to 10.83% (pCramoll) and 24.17% (rCramoll). Lectins treatment attenuated the excessive superoxide production, the collapse of the mitochondrial membrane potential, and the lysosomal and DNA damage in H2O2-treated cells. In conclusion, our results suggest that pCramoll and rCramoll blocked H2O2-induced cytotoxicity through decreasing reactive oxygen species, restoring the mitochondrial potential, preventing the lysosomal damage and DNA fragmentation, and thus promoting cell survival and proliferation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/21004/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL26576224 2015 sil-prc.oa.pdf26576224 2015 sil-prc.oa.pdfapplication/pdf1289207https://www.arca.fiocruz.br/bitstream/icict/21004/2/26576224%202015%20sil-prc.oa.pdf3a0e33cf77fb4987297bc1c4c59ab62eMD52TEXT26576224 2015 sil-prc.oa.pdf.txt26576224 2015 sil-prc.oa.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide |
title |
pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide |
spellingShingle |
pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide da Silva, Luís Cláudio Nascimento Peróxido de Hidrogênio Lectinas Estresse Oxidativo Substâncias Protetoras Animals Apoptosis Cell Proliferation Cercopithecus aethiops DNA Damage Fabaceae Hydrogen Peroxide Lectins Mitochondria/ Oxidative Stress Protective Agents Reactive Oxygen Species Recombinant Proteins Seeds Vero Cells Peróxido de Hidrogênio Lectinas Estresse Oxidativo Substâncias Protetoras Animais Proliferação Celular Apoptose Cercopithecus aethiops Dano ao DNA Fabaceae Potencial da Membrana Mitocondrial Mitocôndrias Espécies Reativas de Oxigênio Proteínas Recombinantes Sementes Células Vero |
title_short |
pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide |
title_full |
pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide |
title_fullStr |
pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide |
title_full_unstemmed |
pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide |
title_sort |
pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide |
author |
da Silva, Luís Cláudio Nascimento |
author_facet |
da Silva, Luís Cláudio Nascimento Alves, Neyla Maria Pereira de Castro, Maria Carolina Accioly Brelaz Higino, Taciana Mirely Maciel da Cunha, Cássia Regina Albuquerque Pereira, Valéria Rêgo Alves da Paz, Nathalia Varejão Nogueira Coelho, Luana Cassandra Breitenbach Barroso Correia, Maria Tereza dos Santos de Figueiredo, Regina Celia Bressan Queiroz |
author_role |
author |
author2 |
Alves, Neyla Maria Pereira de Castro, Maria Carolina Accioly Brelaz Higino, Taciana Mirely Maciel da Cunha, Cássia Regina Albuquerque Pereira, Valéria Rêgo Alves da Paz, Nathalia Varejão Nogueira Coelho, Luana Cassandra Breitenbach Barroso Correia, Maria Tereza dos Santos de Figueiredo, Regina Celia Bressan Queiroz |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
da Silva, Luís Cláudio Nascimento Alves, Neyla Maria Pereira de Castro, Maria Carolina Accioly Brelaz Higino, Taciana Mirely Maciel da Cunha, Cássia Regina Albuquerque Pereira, Valéria Rêgo Alves da Paz, Nathalia Varejão Nogueira Coelho, Luana Cassandra Breitenbach Barroso Correia, Maria Tereza dos Santos de Figueiredo, Regina Celia Bressan Queiroz |
dc.subject.other.pt_BR.fl_str_mv |
Peróxido de Hidrogênio Lectinas Estresse Oxidativo Substâncias Protetoras |
topic |
Peróxido de Hidrogênio Lectinas Estresse Oxidativo Substâncias Protetoras Animals Apoptosis Cell Proliferation Cercopithecus aethiops DNA Damage Fabaceae Hydrogen Peroxide Lectins Mitochondria/ Oxidative Stress Protective Agents Reactive Oxygen Species Recombinant Proteins Seeds Vero Cells Peróxido de Hidrogênio Lectinas Estresse Oxidativo Substâncias Protetoras Animais Proliferação Celular Apoptose Cercopithecus aethiops Dano ao DNA Fabaceae Potencial da Membrana Mitocondrial Mitocôndrias Espécies Reativas de Oxigênio Proteínas Recombinantes Sementes Células Vero |
dc.subject.en.pt_BR.fl_str_mv |
Animals Apoptosis Cell Proliferation Cercopithecus aethiops DNA Damage Fabaceae Hydrogen Peroxide Lectins Mitochondria/ Oxidative Stress Protective Agents Reactive Oxygen Species Recombinant Proteins Seeds Vero Cells |
dc.subject.decs.pt_BR.fl_str_mv |
Peróxido de Hidrogênio Lectinas Estresse Oxidativo Substâncias Protetoras Animais Proliferação Celular Apoptose Cercopithecus aethiops Dano ao DNA Fabaceae Potencial da Membrana Mitocondrial Mitocôndrias Espécies Reativas de Oxigênio Proteínas Recombinantes Sementes Células Vero |
description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2017-09-13T18:45:46Z |
dc.date.available.fl_str_mv |
2017-09-13T18:45:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DA SILVA, L. C. N. et al. pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide. Oxidative Medicine and Cellular Longevity, v. 2015, p. 520872, 2015. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/21004 |
dc.identifier.issn.pt_BR.fl_str_mv |
1942-0994 |
dc.identifier.eissn.none.fl_str_mv |
10.1155/2015/520872 |
identifier_str_mv |
DA SILVA, L. C. N. et al. pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide. Oxidative Medicine and Cellular Longevity, v. 2015, p. 520872, 2015. 1942-0994 10.1155/2015/520872 |
url |
https://www.arca.fiocruz.br/handle/icict/21004 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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