Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13383 |
Resumo: | Ministério da Saúde. Instituto Nacional de Saúde. Laboratório Nacional de Referência de Microbiologia. Maputo, Mozambique. |
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Nhantumbo, Aquino AlbinoCantarelli, Vlademir VicenteCaireão, JulianaMunguambe, Alcides MonizComé, Charlotte ElizabethPinto, Gabriela do CarmoZimba, Tomás FranciscoMandomando, InácioSemá, Cynthia BaltazarDias, CíceroMoraes, Milton OzórioGudo, Eduardo Samo2016-03-29T14:10:33Z2016-03-29T14:10:33Z2015NHANTUMBO, Aquino Albino; et al. Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease. Plos One, v.10, n.9, e0138249, Sept. 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1338310.1371/journal.pone.0138249engPublic Library of ScienceFrequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMinistério da Saúde. Instituto Nacional de Saúde. Laboratório Nacional de Referência de Microbiologia. Maputo, Mozambique.Universidade Feevale. Rio Sul, RS, Brasil / Universidade Federal de Ciências de Saúde de Porto Alegre (UFCSPA). Porto Alegre, RS, Brasil.Universidade Federal de Ciências de Saúde de Porto Alegre (UFCSPA). Porto Alegre, RS, Brasil.Ministério da Saúde. Instituto Nacional de Saúde. Laboratório Nacional de Referência de Microbiologia. Maputo, Mozambique.Ministério da Saúde. Instituto Nacional de Saúde. Laboratório Nacional de Referência de Microbiologia. Maputo, Mozambique.Ministério da Saúde. Instituto Nacional de Saúde. Laboratório de Isolamento Viral. Maputo, Moazambique.Ministério da Saúde. Departamento de Medicina at the Hospital Central de Maputo. Maputo, Mozambique.Ministério da Saúde. Centro de Investigação em Saúde da Manhiça. Maputo, Mozambique.Ministério da Saúde. Instituto Nacional de Saúde. Maputo, Mozambique.Universidade Federal de Ciências de Saúde de Porto Alegre (UFCSPA). Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Instituto Nacional de Saúde. Maputo, Mozambique.BACKGROUND: In Sub-Saharan Africa, including Mozambique, acute bacterial meningitis (ABM) represents a main cause of childhood mortality. The burden of ABM is seriously underestimated because of the poor performance of culture sampling, the primary method of ABM surveillance in the region. Low quality cerebrospinal fluid (CSF) samples and frequent consumption of antibiotics prior to sample collection lead to a high rate of false-negative results. To our knowledge, this study is the first to determine the frequency of ABM in Mozambique using real-time polymerase chain reaction (qPCR) and to compare results to those of culture sampling. METHOD: Between March 2013 and March 2014, CSF samples were collected at 3 regional hospitals from patients under 5 years of age, who met World Health Organization case definition criteria for ABM. Macroscopic examination, cytochemical study, culture, and qPCR were performed on all samples. RESULTS: A total of 369 CSF samples were collected from children clinically suspected of ABM. qPCR showed a significantly higher detection rate of ABM-causing pathogens when compared to culture (52.3% [193/369] versus 7.3% [27/369], p = 0.000). The frequency of Streptococcus pneumoniae, Haemophilus influenzae, group B Streptococci, and Neisseria meningitidis were 32.8% (121⁄369), 12.2%, (45⁄369), 3.0% (16⁄369) and 4.3% (11⁄369), respectively, significantly higher compared to that obtained on culture (p < 0.001 for each). CONCLUSION: Our findings demonstrate that culture is less effective for the diagnosis of ABM than qPCR. The common use of culture rather than qPCR to identify ABM results in serious underestimation of the burden of the disease, and our findings strongly suggest that qPCR should be incorporated into surveillance activities for ABM. In addition, our data showed that S. pneumoniae represents the most common cause of ABM in children under 5 years of age.MeningitisMozambiqueBurden of DiseaseMultiplex Polymerase Chain ReactionMeningiteEfeitos Psicossociais da DoençaReação em Cadeia da Polimerase MultiplexMoçambiqueinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13383/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmilton_moraes_etal_IOC_2015.pdfmilton_moraes_etal_IOC_2015.pdfapplication/pdf1045840https://www.arca.fiocruz.br/bitstream/icict/13383/2/milton_moraes_etal_IOC_2015.pdfff68fd24b3e235f6323cb9332fb96d88MD52TEXTmilton_moraes_etal_IOC_2015.pdf.txtmilton_moraes_etal_IOC_2015.pdf.txtExtracted 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Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease |
| title |
Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease |
| spellingShingle |
Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease Nhantumbo, Aquino Albino Meningitis Mozambique Burden of Disease Multiplex Polymerase Chain Reaction Meningite Efeitos Psicossociais da Doença Reação em Cadeia da Polimerase Multiplex Moçambique |
| title_short |
Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease |
| title_full |
Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease |
| title_fullStr |
Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease |
| title_full_unstemmed |
Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease |
| title_sort |
Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease |
| author |
Nhantumbo, Aquino Albino |
| author_facet |
Nhantumbo, Aquino Albino Cantarelli, Vlademir Vicente Caireão, Juliana Munguambe, Alcides Moniz Comé, Charlotte Elizabeth Pinto, Gabriela do Carmo Zimba, Tomás Francisco Mandomando, Inácio Semá, Cynthia Baltazar Dias, Cícero Moraes, Milton Ozório Gudo, Eduardo Samo |
| author_role |
author |
| author2 |
Cantarelli, Vlademir Vicente Caireão, Juliana Munguambe, Alcides Moniz Comé, Charlotte Elizabeth Pinto, Gabriela do Carmo Zimba, Tomás Francisco Mandomando, Inácio Semá, Cynthia Baltazar Dias, Cícero Moraes, Milton Ozório Gudo, Eduardo Samo |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nhantumbo, Aquino Albino Cantarelli, Vlademir Vicente Caireão, Juliana Munguambe, Alcides Moniz Comé, Charlotte Elizabeth Pinto, Gabriela do Carmo Zimba, Tomás Francisco Mandomando, Inácio Semá, Cynthia Baltazar Dias, Cícero Moraes, Milton Ozório Gudo, Eduardo Samo |
| dc.subject.en.pt_BR.fl_str_mv |
Meningitis Mozambique Burden of Disease Multiplex Polymerase Chain Reaction |
| topic |
Meningitis Mozambique Burden of Disease Multiplex Polymerase Chain Reaction Meningite Efeitos Psicossociais da Doença Reação em Cadeia da Polimerase Multiplex Moçambique |
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Meningite Efeitos Psicossociais da Doença Reação em Cadeia da Polimerase Multiplex Moçambique |
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Ministério da Saúde. Instituto Nacional de Saúde. Laboratório Nacional de Referência de Microbiologia. Maputo, Mozambique. |
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2015 |
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2015 |
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2016-03-29T14:10:33Z |
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NHANTUMBO, Aquino Albino; et al. Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease. Plos One, v.10, n.9, e0138249, Sept. 2015. |
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1932-6203 |
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10.1371/journal.pone.0138249 |
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NHANTUMBO, Aquino Albino; et al. Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease. Plos One, v.10, n.9, e0138249, Sept. 2015. 1932-6203 10.1371/journal.pone.0138249 |
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