Rab7 controls lipid droplet-phagosome association during mycobacterial infection
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44773 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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Roque, Natalia R.Lage, Sivia L.Navarro, RobertaFazolini, NarayanaMonteiro, Clarissa M. MayaRiedorf, JensMelo, Rossana C. N.D'Avila, HeloisaBozza, Patrícia T.2020-12-09T20:00:15Z2020-12-09T20:00:15Z2020ROQUE, Natalia R. et al. Rab7 controls lipid droplet-phagosome association during mycobacterial infection. Biochimica et Piophysica Acta (BBA) - Molecular and Cell Biology of Lipids, v. 1865, n. 8, 158703, Mar. 2020.1388-1981https://www.arca.fiocruz.br/handle/icict/4477310.1016/j.bbalip.2020.158703engElsevierLeucemia Basofílica AgudaGotículas LipídicasFagossomosM. bovis BCGLAMLipid dropletMycobacteriumPhagosomesRabM. bovis BCGGotículas LipídicasLeucemia Basofílica AgudaComplexo Mycobacterium aviumFagossomosProteína rab2 de Ligação ao GTPRab7 controls lipid droplet-phagosome association during mycobacterial infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil;Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratóro de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratóro de Biologia Celular. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Lipid droplets (LDs) are organelles that have multiple roles in inflammatory and infectious diseases. LD act as essential platforms for immunometabolic regulation, including as sites for lipid storage and metabolism, inflammatory lipid mediator production, and signaling pathway compartmentalization. Accumulating evidence indicates that intracellular pathogens may exploit host LDs as source of nutrients and as part of their strategy to promote immune evasion. Notably, numerous studies have demonstrated the interaction between LDs and pathogen-containing phagosomes. However, the mechanism involved in this phenomenon remains elusive. Here, we observed LDs and PLIN2 surrounding M. bovis BCG-containing phagosomes, which included observations of a bacillus cell surrounded by lipid content inside a phagosome and LAM from mycobacteria co-localizing with LDs; these results were suggestive of exchange of contents between these compartments. By using beads coated with M.tb lipids, we demonstrated that LD-phagosome associations are regulated through the mycobacterial cell wall components LAM and PIM. In addition, we demonstrated that Rab7 and RILP, but not Rab5, localizes to LDs of infected macrophages and observed the presence of Rab7 at the site of interaction with an infected phagosome. Moreover, treatment of macrophages with the Rab7 inhibitor CID1067700 significantly inhibited the association between LDs and LAM-coated beads. Altogether, our data demonstrate that LD-phagosome interactions are controlled by mycobacterial cell wall components and Rab7, which enables the exchange of contents between LDs and phagosomes and may represent a fundamental aspect of bacterial pathogenesis and immune evasion.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44773/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNataliaRoque_`PatriciaBozza_etal-IOC_2020.pdfNataliaRoque_`PatriciaBozza_etal-IOC_2020.pdfapplication/pdf4228991https://www.arca.fiocruz.br/bitstream/icict/44773/2/NataliaRoque_%60PatriciaBozza_etal-IOC_2020.pdfef5adeb199b77324a2a229fe90a309edMD52TEXTNataliaRoque_`PatriciaBozza_etal-IOC_2020.pdf.txtNataliaRoque_`PatriciaBozza_etal-IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Rab7 controls lipid droplet-phagosome association during mycobacterial infection |
title |
Rab7 controls lipid droplet-phagosome association during mycobacterial infection |
spellingShingle |
Rab7 controls lipid droplet-phagosome association during mycobacterial infection Roque, Natalia R. Leucemia Basofílica Aguda Gotículas Lipídicas Fagossomos M. bovis BCG LAM Lipid droplet Mycobacterium Phagosomes Rab M. bovis BCG Gotículas Lipídicas Leucemia Basofílica Aguda Complexo Mycobacterium avium Fagossomos Proteína rab2 de Ligação ao GTP |
title_short |
Rab7 controls lipid droplet-phagosome association during mycobacterial infection |
title_full |
Rab7 controls lipid droplet-phagosome association during mycobacterial infection |
title_fullStr |
Rab7 controls lipid droplet-phagosome association during mycobacterial infection |
title_full_unstemmed |
Rab7 controls lipid droplet-phagosome association during mycobacterial infection |
title_sort |
Rab7 controls lipid droplet-phagosome association during mycobacterial infection |
author |
Roque, Natalia R. |
author_facet |
Roque, Natalia R. Lage, Sivia L. Navarro, Roberta Fazolini, Narayana Monteiro, Clarissa M. Maya Riedorf, Jens Melo, Rossana C. N. D'Avila, Heloisa Bozza, Patrícia T. |
author_role |
author |
author2 |
Lage, Sivia L. Navarro, Roberta Fazolini, Narayana Monteiro, Clarissa M. Maya Riedorf, Jens Melo, Rossana C. N. D'Avila, Heloisa Bozza, Patrícia T. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Roque, Natalia R. Lage, Sivia L. Navarro, Roberta Fazolini, Narayana Monteiro, Clarissa M. Maya Riedorf, Jens Melo, Rossana C. N. D'Avila, Heloisa Bozza, Patrícia T. |
dc.subject.other.pt_BR.fl_str_mv |
Leucemia Basofílica Aguda Gotículas Lipídicas Fagossomos M. bovis BCG |
topic |
Leucemia Basofílica Aguda Gotículas Lipídicas Fagossomos M. bovis BCG LAM Lipid droplet Mycobacterium Phagosomes Rab M. bovis BCG Gotículas Lipídicas Leucemia Basofílica Aguda Complexo Mycobacterium avium Fagossomos Proteína rab2 de Ligação ao GTP |
dc.subject.en.pt_BR.fl_str_mv |
LAM Lipid droplet Mycobacterium Phagosomes Rab M. bovis BCG |
dc.subject.decs.pt_BR.fl_str_mv |
Gotículas Lipídicas Leucemia Basofílica Aguda Complexo Mycobacterium avium Fagossomos Proteína rab2 de Ligação ao GTP |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-12-09T20:00:15Z |
dc.date.available.fl_str_mv |
2020-12-09T20:00:15Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ROQUE, Natalia R. et al. Rab7 controls lipid droplet-phagosome association during mycobacterial infection. Biochimica et Piophysica Acta (BBA) - Molecular and Cell Biology of Lipids, v. 1865, n. 8, 158703, Mar. 2020. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/44773 |
dc.identifier.issn.pt_BR.fl_str_mv |
1388-1981 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.bbalip.2020.158703 |
identifier_str_mv |
ROQUE, Natalia R. et al. Rab7 controls lipid droplet-phagosome association during mycobacterial infection. Biochimica et Piophysica Acta (BBA) - Molecular and Cell Biology of Lipids, v. 1865, n. 8, 158703, Mar. 2020. 1388-1981 10.1016/j.bbalip.2020.158703 |
url |
https://www.arca.fiocruz.br/handle/icict/44773 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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