A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide

Detalhes bibliográficos
Autor(a) principal: Pinho, Rosa Teixeira de
Data de Publicação: 1991
Outros Autores: Dutra, Helio dos Santos, De Simone, Salvatore Giovanni, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8342
Resumo: WHO Collaborating Center on Immunology of Parasitic Diseases. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
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spelling Pinho, Rosa Teixeira deDutra, Helio dos SantosDe Simone, Salvatore GiovanniPontes-de-Carvalho, Lain Carlos2014-09-10T14:17:25Z2014-09-10T14:17:25Z1991PINHO, Rosa Teixeira de et al. A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide. Acta Tropica, v. 50, n. 1, p. 29-38, 1991.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/8342engElsevier Science Publishers B.VA glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptideinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleWHO Collaborating Center on Immunology of Parasitic Diseases. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.WHO Collaborating Center on Immunology of Parasitic Diseases. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niteroi, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Glass wool, hydrophilic cotton wool, non-electrically charged BIO-GEL P2 and common tissue paper columns were used to purify trypomastigotes from a mixed Trypanosoma cruzi population grown in axenic culture medium. With all these columns, highly purified (up to 98%) trypomastigote preparations were obtained. Trypomastigote yields from cotton wool, BIO-GEL P2 and common tissue paper columns were not as high as from glass wool columns, from which yields varied from 69 to 80%. Purification on glass wool did not affect trypomastigote infectivity or virulence. Dead trypomastigotes could not be purified on glass wool columns. A glass-adherent amphiphilic peptide of 45 kDa, present in the cell membrane, was isolated from epimastigote but not from trypomastigote preparations.Trypanosoma cruziGlass wool purificationTrypomastigotesSurface peptideTriton X-114Doença de Chagas/parasitologiaVidroProteínas de Membrana/análiseProteínas de Protozoários/análiseTrypanosoma cruzi/isolamento & purificaçãoAnimaisCromatografia DEAE-CeluloseEletroforese em Gel de PoliacrilamidaInsetos Vetores/parasitologiaMasculinoProteínas de Membrana/metabolismoCamundongosProteínas de Protozoários/metabolismoTriatoma/parasitologiaTrypanosoma cruzi/químicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8342/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALPinho RT A glass wool-based....pdfPinho RT A glass wool-based....pdfapplication/pdf774453https://www.arca.fiocruz.br/bitstream/icict/8342/2/Pinho%20RT%20A%20glass%20wool-based....pdf72ff80be71df1cee97a4e7497caa52fbMD52TEXTPinho RT A glass wool-based....pdf.txtPinho RT A glass wool-based....pdf.txtExtracted texttext/plain25673https://www.arca.fiocruz.br/bitstream/icict/8342/3/Pinho%20RT%20A%20glass%20wool-based....pdf.txt179c9fdf0b1a59370d0d8c082ca52b04MD53icict/83422023-09-14 15:53:38.258oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T18:53:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
title A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
spellingShingle A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
Pinho, Rosa Teixeira de
Trypanosoma cruzi
Glass wool purification
Trypomastigotes
Surface peptide
Triton X-114
Doença de Chagas/parasitologia
Vidro
Proteínas de Membrana/análise
Proteínas de Protozoários/análise
Trypanosoma cruzi/isolamento & purificação
Animais
Cromatografia DEAE-Celulose
Eletroforese em Gel de Poliacrilamida
Insetos Vetores/parasitologia
Masculino
Proteínas de Membrana/metabolismo
Camundongos
Proteínas de Protozoários/metabolismo
Triatoma/parasitologia
Trypanosoma cruzi/química
title_short A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
title_full A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
title_fullStr A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
title_full_unstemmed A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
title_sort A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
author Pinho, Rosa Teixeira de
author_facet Pinho, Rosa Teixeira de
Dutra, Helio dos Santos
De Simone, Salvatore Giovanni
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Dutra, Helio dos Santos
De Simone, Salvatore Giovanni
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
author
dc.contributor.author.fl_str_mv Pinho, Rosa Teixeira de
Dutra, Helio dos Santos
De Simone, Salvatore Giovanni
Pontes-de-Carvalho, Lain Carlos
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Glass wool purification
Trypomastigotes
Surface peptide
Triton X-114
topic Trypanosoma cruzi
Glass wool purification
Trypomastigotes
Surface peptide
Triton X-114
Doença de Chagas/parasitologia
Vidro
Proteínas de Membrana/análise
Proteínas de Protozoários/análise
Trypanosoma cruzi/isolamento & purificação
Animais
Cromatografia DEAE-Celulose
Eletroforese em Gel de Poliacrilamida
Insetos Vetores/parasitologia
Masculino
Proteínas de Membrana/metabolismo
Camundongos
Proteínas de Protozoários/metabolismo
Triatoma/parasitologia
Trypanosoma cruzi/química
dc.subject.decs.pt_BR.fl_str_mv Doença de Chagas/parasitologia
Vidro
Proteínas de Membrana/análise
Proteínas de Protozoários/análise
Trypanosoma cruzi/isolamento & purificação
Animais
Cromatografia DEAE-Celulose
Eletroforese em Gel de Poliacrilamida
Insetos Vetores/parasitologia
Masculino
Proteínas de Membrana/metabolismo
Camundongos
Proteínas de Protozoários/metabolismo
Triatoma/parasitologia
Trypanosoma cruzi/química
description WHO Collaborating Center on Immunology of Parasitic Diseases. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
publishDate 1991
dc.date.issued.fl_str_mv 1991
dc.date.accessioned.fl_str_mv 2014-09-10T14:17:25Z
dc.date.available.fl_str_mv 2014-09-10T14:17:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PINHO, Rosa Teixeira de et al. A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide. Acta Tropica, v. 50, n. 1, p. 29-38, 1991.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8342
dc.identifier.issn.none.fl_str_mv 0001-706X
identifier_str_mv PINHO, Rosa Teixeira de et al. A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide. Acta Tropica, v. 50, n. 1, p. 29-38, 1991.
0001-706X
url https://www.arca.fiocruz.br/handle/icict/8342
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Science Publishers B.V
publisher.none.fl_str_mv Elsevier Science Publishers B.V
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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