Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form

Detalhes bibliográficos
Autor(a) principal: Neves, Antonio Pereira
Data de Publicação: 2015
Outros Autores: Gonzaga, Luiz, Menna-Barreto, Rubem F. S., Benchimol, Marlene
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13056
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Programa de Pós-Graduação em Ciências Morfológicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Aggeu Magalhães. Departamento de Microbiologia. Laboratório de Microbiologia e Biologia Celular. Recife, PE, Brasil.
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spelling Neves, Antonio PereiraGonzaga, LuizMenna-Barreto, Rubem F. S.Benchimol, Marlene2016-03-08T14:59:36Z2016-03-08T14:59:36Z2015NEVES, Antonio Pereira et al. Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form. Plos One, v. 10, n. 8, p. 1-32, June 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1305610.1371/journal.pone.0129165engPublic Library of ScienceCharacterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Forminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Programa de Pós-Graduação em Ciências Morfológicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Aggeu Magalhães. Departamento de Microbiologia. Laboratório de Microbiologia e Biologia Celular. Recife, PE, Brasil.Laboratório Nacional de Computação Cientifica. Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Universidade do Grande Rio. Duque de Caxias, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Proteasomes are intracellular complexes that control selective protein degradation in organisms ranging from Archaea to higher eukaryotes. These structures have multiple proteolytic activities that are required for cell differentiation, replication and maintaining cellular homeostasis. Here, we document the presence of the 20S proteasome in the protist parasite Tritrichomonas foetus. Complementary techniques, such as a combination of whole genome sequencing technologies, bioinformatics algorithms, cell fractionation and biochemistry and microscopy approaches were used to characterise the 20S proteasome of T. foetus. The 14 homologues of the typical eukaryotic proteasome subunits were identified in the T. foetus genome. Alignment analyses showed that the main regulatory and catalytic domains of the proteasome were conserved in the predicted amino acid sequences from T. foetus-proteasome subunits. Immunofluorescence assays using an anti-proteasome antibody revealed a labelling distributed throughout the cytosol as punctate cytoplasmic structures and in the perinuclear region. Electron microscopy of a T. foetus-proteasome-enriched fraction confirmed the presence of particles that resembled the typical eukaryotic 20S proteasome. Fluorogenic assays using specific peptidyl substrates detected presence of the three typical peptidase activities of eukaryotic proteasomes in T. foetus. As expected, these peptidase activities were inhibited by lactacystin, a well-known specific proteasome inhibitor, and were not affected by inhibitors of serine or cysteine proteases. During the transformation of T. foetus to endoflagellar form (EFF), also known as pseudocyst, we observed correlations between the EFF formation rates, increases in the proteasome activities and reduced levels of ubiquitin-protein conjugates. The growth, cell cycle and EFF transformation of T. foetus were inhibited after treatment with lactacystin in a dose-dependent manner. Lactacystin treatment also resulted in an accumulation of ubiquitinated proteins and caused increase in the amount of endoplasmic reticulum membranes in the parasite. Taken together, our results suggest that the ubiquitin-proteasome pathway is required for cell cycle and EFF transformation in T. foetus.ProteasomeTritrichomonas foetusCell cycleEndoflagellar form (EFF)ProteasomeTritrichomonas foetusCiclo Celularinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13056/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALantonio_neves_etal_IOC_2015.pdfantonio_neves_etal_IOC_2015.pdfapplication/pdf13265975https://www.arca.fiocruz.br/bitstream/icict/13056/2/antonio_neves_etal_IOC_2015.pdf744078c2154cd15d78220568fd5f29cdMD52TEXTantonio_neves_etal_IOC_2015.pdf.txtantonio_neves_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form
title Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form
spellingShingle Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form
Neves, Antonio Pereira
Proteasome
Tritrichomonas foetus
Cell cycle
Endoflagellar form (EFF)
Proteasome
Tritrichomonas foetus
Ciclo Celular
title_short Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form
title_full Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form
title_fullStr Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form
title_full_unstemmed Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form
title_sort Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form
author Neves, Antonio Pereira
author_facet Neves, Antonio Pereira
Gonzaga, Luiz
Menna-Barreto, Rubem F. S.
Benchimol, Marlene
author_role author
author2 Gonzaga, Luiz
Menna-Barreto, Rubem F. S.
Benchimol, Marlene
author2_role author
author
author
dc.contributor.author.fl_str_mv Neves, Antonio Pereira
Gonzaga, Luiz
Menna-Barreto, Rubem F. S.
Benchimol, Marlene
dc.subject.en.pt_BR.fl_str_mv Proteasome
Tritrichomonas foetus
Cell cycle
Endoflagellar form (EFF)
topic Proteasome
Tritrichomonas foetus
Cell cycle
Endoflagellar form (EFF)
Proteasome
Tritrichomonas foetus
Ciclo Celular
dc.subject.decs.pt_BR.fl_str_mv Proteasome
Tritrichomonas foetus
Ciclo Celular
description Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Programa de Pós-Graduação em Ciências Morfológicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Aggeu Magalhães. Departamento de Microbiologia. Laboratório de Microbiologia e Biologia Celular. Recife, PE, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-08T14:59:36Z
dc.date.available.fl_str_mv 2016-03-08T14:59:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NEVES, Antonio Pereira et al. Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form. Plos One, v. 10, n. 8, p. 1-32, June 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13056
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0129165
identifier_str_mv NEVES, Antonio Pereira et al. Characterisation of 20S Proteasome in Tritrichomonas foetus and Its Role during the Cell Cycle and Transformation into Endoflagellar Form. Plos One, v. 10, n. 8, p. 1-32, June 2015.
1932-6203
10.1371/journal.pone.0129165
url https://www.arca.fiocruz.br/handle/icict/13056
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/13056/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/13056/2/antonio_neves_etal_IOC_2015.pdf
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