Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers

Detalhes bibliográficos
Autor(a) principal: Bianco, Kayo
Data de Publicação: 2015
Outros Autores: Barreto, Camila, Oliveira, Samara Sant’Anna, Pinto, Leonardo Henriques, Albano, Rodolpho Mattos, Miranda, Catia Chaia, Clementino, Maysa Mandetta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/52760
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.
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spelling Bianco, KayoBarreto, CamilaOliveira, Samara Sant’AnnaPinto, Leonardo HenriquesAlbano, Rodolpho MattosMiranda, Catia ChaiaClementino, Maysa Mandetta2022-05-18T17:38:04Z2022-05-18T17:38:04Z2015BIANCO, Kayo et al. Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers. Journal of Water and Health, v. 13, n. 4, p. 985-995, 2015.1996-7829https://www.arca.fiocruz.br/handle/icict/5276010.2166/wh.2015.292engBacteroidetesBiomarkersDrinking WaterFecesLimit of DetectionMethanobrevibacterPolymerase Chain ReactionRNA, Ribosomal, 16SSensitivity and SpecificityBacteroidalesFecal pollutionMethanobrevibacter spp.Microbial source trackingWater qualityBacteroidetesBiomarcadoresÁgua PotávelFezesLimite de DetecçãoMethanobrevibacterReação em Cadeia da PolimeraseRNA Ribossômico 16SSensibilidade e EspecificidadeFecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Rio de Janeiro. Centro Biomédico do Hospital Universitário Antônio Pedro. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Rio de Janeiro. Centro Biomédico do Hospital Universitário Antônio Pedro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.The determination of fecal pollution sources in aquatic ecosystems is essential to estimate associated health risks. In this study, we evaluate eight microbial source tracking (MST) markers including host-specific Bacteroidales and Methanobrevibacter spp. for discrimination between human, bovine, equine, and swine fecal contamination in waters intended for human supply. Overall, the novel host-specific archaeal and bacterial primers proposed in this study demonstrated high sensitivity and specificity. Markers for the Archaea domain were more prevalent in the fecal and water samples studied. We conclude that the investigations regarding the sources of fecal pollution in public water supplies can contribute to improve the quality of human health. To our knowledge, this is the first analysis using both archaeal and bacterial fecal MST markers on tropical water bodies of Rio de Janeiro city, Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83106https://www.arca.fiocruz.br/bitstream/icict/52760/1/license.txtc37b22128114f4f0d76b048c416c4594MD51ORIGINALjwh0130985.pdfjwh0130985.pdfapplication/pdf407011https://www.arca.fiocruz.br/bitstream/icict/52760/2/jwh0130985.pdf627601e70dbd541e05bd58368e36bc37MD52icict/527602022-06-03 00:19:07.035oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-03T03:19:07Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.pt_BR.fl_str_mv Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers
title Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers
spellingShingle Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers
Bianco, Kayo
Bacteroidetes
Biomarkers
Drinking Water
Feces
Limit of Detection
Methanobrevibacter
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Sensitivity and Specificity
Bacteroidales
Fecal pollution
Methanobrevibacter spp.
Microbial source tracking
Water quality
Bacteroidetes
Biomarcadores
Água Potável
Fezes
Limite de Detecção
Methanobrevibacter
Reação em Cadeia da Polimerase
RNA Ribossômico 16S
Sensibilidade e Especificidade
title_short Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers
title_full Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers
title_fullStr Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers
title_full_unstemmed Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers
title_sort Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers
author Bianco, Kayo
author_facet Bianco, Kayo
Barreto, Camila
Oliveira, Samara Sant’Anna
Pinto, Leonardo Henriques
Albano, Rodolpho Mattos
Miranda, Catia Chaia
Clementino, Maysa Mandetta
author_role author
author2 Barreto, Camila
Oliveira, Samara Sant’Anna
Pinto, Leonardo Henriques
Albano, Rodolpho Mattos
Miranda, Catia Chaia
Clementino, Maysa Mandetta
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bianco, Kayo
Barreto, Camila
Oliveira, Samara Sant’Anna
Pinto, Leonardo Henriques
Albano, Rodolpho Mattos
Miranda, Catia Chaia
Clementino, Maysa Mandetta
dc.subject.mesh.pt_BR.fl_str_mv Bacteroidetes
Biomarkers
Drinking Water
Feces
Limit of Detection
Methanobrevibacter
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Sensitivity and Specificity
topic Bacteroidetes
Biomarkers
Drinking Water
Feces
Limit of Detection
Methanobrevibacter
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Sensitivity and Specificity
Bacteroidales
Fecal pollution
Methanobrevibacter spp.
Microbial source tracking
Water quality
Bacteroidetes
Biomarcadores
Água Potável
Fezes
Limite de Detecção
Methanobrevibacter
Reação em Cadeia da Polimerase
RNA Ribossômico 16S
Sensibilidade e Especificidade
dc.subject.en.pt_BR.fl_str_mv Bacteroidales
Fecal pollution
Methanobrevibacter spp.
Microbial source tracking
Water quality
dc.subject.decs.pt_BR.fl_str_mv Bacteroidetes
Biomarcadores
Água Potável
Fezes
Limite de Detecção
Methanobrevibacter
Reação em Cadeia da Polimerase
RNA Ribossômico 16S
Sensibilidade e Especificidade
description Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2022-05-18T17:38:04Z
dc.date.available.fl_str_mv 2022-05-18T17:38:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BIANCO, Kayo et al. Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers. Journal of Water and Health, v. 13, n. 4, p. 985-995, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/52760
dc.identifier.issn.pt_BR.fl_str_mv 1996-7829
dc.identifier.doi.none.fl_str_mv 10.2166/wh.2015.292
identifier_str_mv BIANCO, Kayo et al. Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers. Journal of Water and Health, v. 13, n. 4, p. 985-995, 2015.
1996-7829
10.2166/wh.2015.292
url https://www.arca.fiocruz.br/handle/icict/52760
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/52760/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/52760/2/jwh0130985.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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