The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121594 https://doi.org/10.1186/s40104-019-0422-x |
Resumo: | The success of different species of ruminants in the colonization of a diverse range of environments is due to their ability to digest and absorb nutrients from cellulose, a complex polysaccharide found in leaves and grass. Ruminants rely on a complex and diverse microbial community, or microbiota, in a unique compartment known as the rumen to break down this polysaccharide. Changes in microbial populations of the rumen can affect the host?s development, health, and productivity. However, accessing the rumen is stressful for the animal. Therefore, the development and use of alternative sampling methods are needed if this technique is to be routinely used in cattle breeding. To this end, we tested if the fecal microbiome could be used as a proxy for the rumen microbiome due to its accessibility. We investigated the taxonomic composition, diversity and inter-relations of two different GIT compartments, rumen and feces, of 26 Nelore (Bos indicus) bulls, using Next Generation Sequencing (NGS) metabarcoding of bacteria, archaea and ciliate protozoa. |
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The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen.MicrobiotaMetabarcodingBactériaBos IndicusGado NeloreArchaeaMethanobrevibacterMicrobiomeThe success of different species of ruminants in the colonization of a diverse range of environments is due to their ability to digest and absorb nutrients from cellulose, a complex polysaccharide found in leaves and grass. Ruminants rely on a complex and diverse microbial community, or microbiota, in a unique compartment known as the rumen to break down this polysaccharide. Changes in microbial populations of the rumen can affect the host?s development, health, and productivity. However, accessing the rumen is stressful for the animal. Therefore, the development and use of alternative sampling methods are needed if this technique is to be routinely used in cattle breeding. To this end, we tested if the fecal microbiome could be used as a proxy for the rumen microbiome due to its accessibility. We investigated the taxonomic composition, diversity and inter-relations of two different GIT compartments, rumen and feces, of 26 Nelore (Bos indicus) bulls, using Next Generation Sequencing (NGS) metabarcoding of bacteria, archaea and ciliate protozoa.Bruno Gabriel Nascimento Andrade, FAPESP; FLAVIA ALINE BRESSANI DONATONI, CPPSE; Rafael R. C. Cuadrat, German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE); Polyana C. Tizioto, NGS Genomic Solutions; Priscila S. N. de Oliveira, CAPES - UFSCar; Gerson B. Mourão, USP; Luiz L. Coutinho, USP; James M. Reecy, Iowa State University; James E. Koltes, Iowa State University; Paul Walsh, NSilico Life Science; ALEXANDRE BERNDT, CPPSE; JULIO CESAR PASCALE PALHARES, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.ANDRADE, B. G. N.DONATONI, F. A. B.CUADRAT, R. R. C.TIZIOTO, P. C.OLIVEIRA, P. S. N. deMOURÃO, G. B.COUTINHO, L. L.REECY, J. M.KOLTES, J. E.WALSH, P.BERNDT, A.PALHARES, J. C. P.REGITANO, L. C. de A.2020-04-09T00:50:04Z2020-04-09T00:50:04Z2020-04-0820202020-04-20T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10 p.Journal of Animal Science and Biotechnology, v.11, n. 6, 2020.2049-1891http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121594https://doi.org/10.1186/s40104-019-0422-xenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-04-09T00:50:12Zoai:www.alice.cnptia.embrapa.br:doc/1121594Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-04-09T00:50:12falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-04-09T00:50:12Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. |
title |
The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. |
spellingShingle |
The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. ANDRADE, B. G. N. Microbiota Metabarcoding Bactéria Bos Indicus Gado Nelore Archaea Methanobrevibacter Microbiome |
title_short |
The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. |
title_full |
The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. |
title_fullStr |
The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. |
title_full_unstemmed |
The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. |
title_sort |
The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. |
author |
ANDRADE, B. G. N. |
author_facet |
ANDRADE, B. G. N. DONATONI, F. A. B. CUADRAT, R. R. C. TIZIOTO, P. C. OLIVEIRA, P. S. N. de MOURÃO, G. B. COUTINHO, L. L. REECY, J. M. KOLTES, J. E. WALSH, P. BERNDT, A. PALHARES, J. C. P. REGITANO, L. C. de A. |
author_role |
author |
author2 |
DONATONI, F. A. B. CUADRAT, R. R. C. TIZIOTO, P. C. OLIVEIRA, P. S. N. de MOURÃO, G. B. COUTINHO, L. L. REECY, J. M. KOLTES, J. E. WALSH, P. BERNDT, A. PALHARES, J. C. P. REGITANO, L. C. de A. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Bruno Gabriel Nascimento Andrade, FAPESP; FLAVIA ALINE BRESSANI DONATONI, CPPSE; Rafael R. C. Cuadrat, German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE); Polyana C. Tizioto, NGS Genomic Solutions; Priscila S. N. de Oliveira, CAPES - UFSCar; Gerson B. Mourão, USP; Luiz L. Coutinho, USP; James M. Reecy, Iowa State University; James E. Koltes, Iowa State University; Paul Walsh, NSilico Life Science; ALEXANDRE BERNDT, CPPSE; JULIO CESAR PASCALE PALHARES, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
dc.contributor.author.fl_str_mv |
ANDRADE, B. G. N. DONATONI, F. A. B. CUADRAT, R. R. C. TIZIOTO, P. C. OLIVEIRA, P. S. N. de MOURÃO, G. B. COUTINHO, L. L. REECY, J. M. KOLTES, J. E. WALSH, P. BERNDT, A. PALHARES, J. C. P. REGITANO, L. C. de A. |
dc.subject.por.fl_str_mv |
Microbiota Metabarcoding Bactéria Bos Indicus Gado Nelore Archaea Methanobrevibacter Microbiome |
topic |
Microbiota Metabarcoding Bactéria Bos Indicus Gado Nelore Archaea Methanobrevibacter Microbiome |
description |
The success of different species of ruminants in the colonization of a diverse range of environments is due to their ability to digest and absorb nutrients from cellulose, a complex polysaccharide found in leaves and grass. Ruminants rely on a complex and diverse microbial community, or microbiota, in a unique compartment known as the rumen to break down this polysaccharide. Changes in microbial populations of the rumen can affect the host?s development, health, and productivity. However, accessing the rumen is stressful for the animal. Therefore, the development and use of alternative sampling methods are needed if this technique is to be routinely used in cattle breeding. To this end, we tested if the fecal microbiome could be used as a proxy for the rumen microbiome due to its accessibility. We investigated the taxonomic composition, diversity and inter-relations of two different GIT compartments, rumen and feces, of 26 Nelore (Bos indicus) bulls, using Next Generation Sequencing (NGS) metabarcoding of bacteria, archaea and ciliate protozoa. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-09T00:50:04Z 2020-04-09T00:50:04Z 2020-04-08 2020 2020-04-20T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Animal Science and Biotechnology, v.11, n. 6, 2020. 2049-1891 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121594 https://doi.org/10.1186/s40104-019-0422-x |
identifier_str_mv |
Journal of Animal Science and Biotechnology, v.11, n. 6, 2020. 2049-1891 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121594 https://doi.org/10.1186/s40104-019-0422-x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 p. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503491619651584 |