Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Andrade, M. K. N.
Data de Publicação: 2009
Outros Autores: Machado, S. M. A., Leite, M. L., Saad, M. H. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28938
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Doenças do Tórax. Hospital Municipal Raphael de Paula Souza. Rio de Janeiro, RJ, Brasil.
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spelling Andrade, M. K. N.Machado, S. M. A.Leite, M. L.Saad, M. H. F.2018-09-20T14:34:59Z2018-09-20T14:34:59Z2009ANDRADE, M. K. N. et al. Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil. Brazilian Journal of medical and Biological Research, v.42, p.433-437, 2009.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/289381414-431XengAssociação Brasileira de Divulgação CientíficaTuberculoseMycobacterium tuberculosisMutação de GeneImpressão digitalTuberculosisMycobacterium tuberculosisTB-MDRTB-RFLPGene mutationFingerprintPhenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Doenças do Tórax. Hospital Municipal Raphael de Paula Souza. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Doenças do Tórax. Hospital Municipal Raphael de Paula Souza. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Assuming that the IS6110-restriction fragment length polymorphism (RFLP) changes at a constant rate of 3.2 years, this methodology was applied to demonstrate, for the first time, variant patterns of Mycobacterium tuberculosis (MTB) in multiple isolates obtained at short time intervals from sputum and blood of an HIV+ patient with multiple admissions to the Emergency Room and to the multidrug-resistant tuberculosis (MDR-TB) Reference Center of a secondary-care hospital in Rio de Janeiro, Brazil. In sputum, the IS6110-RFLP appeared in isolates with two variant patterns with 10 and 13 IS6110 copies. However, blood presented only the pattern corresponding to 10 copies, suggesting compartmentalization. With regard to the exact match of 10 of 13 bands, this may be a subpopulation with the same clonal origin and this may be related to the IS6110 transposition. A susceptibility test demonstrated an MDR profile (INHR, RIFR, SMR, and EMBR), with the sputum isolate also exhibiting EMBS (R = resistant; S = sensitive). A gene mutation confirmed resistance only to streptomycin. There was agreement between the results of the phenotypic test and the clinical response to MDR-TB treatment, suggesting serious implications with regard to treatment administration based exclusively on molecular methods, and calling attention to the fact that more effective control strategies against the emergence of MDR strains must be implemented by the TB control program to prevent transmission of MDR-MTB strains at health facilities in areas highly endemic for TB.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
title Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
spellingShingle Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
Andrade, M. K. N.
Tuberculose
Mycobacterium tuberculosis
Mutação de Gene
Impressão digital
Tuberculosis
Mycobacterium tuberculosis
TB-MDR
TB-RFLP
Gene mutation
Fingerprint
title_short Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
title_full Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
title_fullStr Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
title_full_unstemmed Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
title_sort Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
author Andrade, M. K. N.
author_facet Andrade, M. K. N.
Machado, S. M. A.
Leite, M. L.
Saad, M. H. F.
author_role author
author2 Machado, S. M. A.
Leite, M. L.
Saad, M. H. F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Andrade, M. K. N.
Machado, S. M. A.
Leite, M. L.
Saad, M. H. F.
dc.subject.other.pt_BR.fl_str_mv Tuberculose
Mycobacterium tuberculosis
Mutação de Gene
Impressão digital
topic Tuberculose
Mycobacterium tuberculosis
Mutação de Gene
Impressão digital
Tuberculosis
Mycobacterium tuberculosis
TB-MDR
TB-RFLP
Gene mutation
Fingerprint
dc.subject.en.pt_BR.fl_str_mv Tuberculosis
Mycobacterium tuberculosis
TB-MDR
TB-RFLP
Gene mutation
Fingerprint
description Universidade Federal do Rio de Janeiro. Instituto de Doenças do Tórax. Hospital Municipal Raphael de Paula Souza. Rio de Janeiro, RJ, Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-09-20T14:34:59Z
dc.date.available.fl_str_mv 2018-09-20T14:34:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANDRADE, M. K. N. et al. Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil. Brazilian Journal of medical and Biological Research, v.42, p.433-437, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28938
dc.identifier.issn.pt_BR.fl_str_mv 0100-879X
dc.identifier.eissn.none.fl_str_mv 1414-431X
identifier_str_mv ANDRADE, M. K. N. et al. Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil. Brazilian Journal of medical and Biological Research, v.42, p.433-437, 2009.
0100-879X
1414-431X
url https://www.arca.fiocruz.br/handle/icict/28938
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/28938/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/28938/2/Sma_machado_etal_IOC_2009.pdf
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