Evaluation of the commercial kit SIRE Nitratase for detecting resistant Mycobacterium tuberculosis in Brazil

• Autor(a) principal: Miranda, Silvana Spindola de
• Data de Publicação: 2017
• Outros Autores: Almeida, Isabela Neves de, Lopes, Maria Luiza, Figueiredo, Jamilly dos Reis de, Figueredo, Lida Jouca de Assis, Kritski, Afrânio Lineu, Carvalho, Wânia da Silva, Mansur, Maria de Fátima Filardi Oliveira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/2870
• topic: Mycobacterium tuberculosis; Mycobacterium tuberculosis / efeitos de drogas; Genes MDR / efeitos de drogas; Nitrato Redutase; Kit SIRE

Introduction: This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility of clinical Mycobacterium tuberculosis isolates. Methods: The accuracy of the Kit SIRE Nitratase was evaluated by examining the susceptibility (streptomycin, isoniazid, rifampicin, and ethambutol) of 40 M. tuberculosis isolates, using the proportion method with Lowenstein-Jensen medium or the BACTEC MGIT 960 system. Results: The detection accuracy for streptomycin, isoniazid, rifampicin, and ethambutol was 95%, 97.5%, 100%, and 80%, respectively. Conclusions: The exceptional accuracy demonstrated by Kit SIRE Nitratase for isoniazid and rifampicin makes the kit an attractive option for screening M. tuberculosis strain resistance.