Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions

Detalhes bibliográficos
Autor(a) principal: Carvalho, B. M. A.
Data de Publicação: 2014
Outros Autores: Carvalho, L. M., Silva Jr. W. F., Minim, L.A., Soares, A. M., Carvalho, G. G. P., Silva, S. L. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13586
Resumo: 2017-11-30
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spelling Carvalho, B. M. A.Carvalho, L. M.Silva Jr. W. F.Minim, L.A.Soares, A. M.Carvalho, G. G. P.Silva, S. L. da2016-04-06T14:41:18Z2014CARVALHO, B. M. A. et al. Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions. Food Chemistry, v. 154, p. 308-314, 2014.https://www.arca.fiocruz.br/handle/icict/1358610.1016/j.foodchem.2014.01.010engElsevierDirect capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2017-11-30Federal University of São João Del Rei. Department of Chemistry, Biotechnology and Bioprocess Engineering. Ouro Branco, MG, Brazil.Federal University of Viçosa. Department of Veterinary. Viçosa, MG, Brazil.Federal University of São João Del Rei. Department of Chemistry, Biotechnology and Bioprocess Engineering. Ouro Branco, MG, Brazil.Federal University of Viçosa. Department of Food Technology. Viçosa, MG, Brazil.Oswaldo Cruz Foundation. Federal University of Rondônia. Porto Velho, RO, Brazil.Federal University of Bahia. Department of Animal Science. Salvador, BA, Brazil.Federal University of São João Del Rei. Department of Chemistry, Biotechnology and Bioprocess Engineering. Ouro Branco, MG, Brazil.Lactoferrin is a protein that is present in cheese whey (a waste product from the dairy industry) and has several biological activities. However, its production from whey must have a high yield and low cost for industrial applications. As such, this study reports the use of polyacrylamide cryogel, loaded with Cu2+ (through the bond with iminodiacetic acid (IDA)), as an adsorbent for the chromatographic process to capture lactoferrin whey. Ultrafiltered cheese whey was passed through the cryogel-IDA-Cu2+ system. The eluates were subjected to analysis of total protein, SDS–PAGE and size exclusion chromatography. The results showed an axial dispersion coefficients, at different superficial velocities of liquid, in a range of 10–6–10–5 m2/s. The cryogel demonstrated good hydraulic permeability (4.7086 _ 10–13 m2) and a porosity of approximately 78.2%. The IDA-Cu2+ cryogel system was also able to capture lactoferrin in high purity.IMAC purificationWhey proteinSupermacroporous cryogelsLactoferrininfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13586/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDirect capture of lactoferrin from cheese.pdfDirect capture of lactoferrin from cheese.pdfapplication/pdf883071https://www.arca.fiocruz.br/bitstream/icict/13586/2/Direct%20capture%20of%20lactoferrin%20from%20cheese.pdf344c25983aa998e8a9f04e1a835d798dMD52TEXTDirect capture of lactoferrin from cheese.pdf.txtDirect capture of lactoferrin from cheese.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions
title Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions
spellingShingle Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions
Carvalho, B. M. A.
IMAC purification
Whey protein
Supermacroporous cryogels
Lactoferrin
title_short Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions
title_full Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions
title_fullStr Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions
title_full_unstemmed Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions
title_sort Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions
author Carvalho, B. M. A.
author_facet Carvalho, B. M. A.
Carvalho, L. M.
Silva Jr. W. F.
Minim, L.A.
Soares, A. M.
Carvalho, G. G. P.
Silva, S. L. da
author_role author
author2 Carvalho, L. M.
Silva Jr. W. F.
Minim, L.A.
Soares, A. M.
Carvalho, G. G. P.
Silva, S. L. da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, B. M. A.
Carvalho, L. M.
Silva Jr. W. F.
Minim, L.A.
Soares, A. M.
Carvalho, G. G. P.
Silva, S. L. da
dc.subject.en.pt_BR.fl_str_mv IMAC purification
Whey protein
Supermacroporous cryogels
Lactoferrin
topic IMAC purification
Whey protein
Supermacroporous cryogels
Lactoferrin
description 2017-11-30
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2016-04-06T14:41:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, B. M. A. et al. Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions. Food Chemistry, v. 154, p. 308-314, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13586
dc.identifier.doi.none.fl_str_mv 10.1016/j.foodchem.2014.01.010
identifier_str_mv CARVALHO, B. M. A. et al. Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions. Food Chemistry, v. 154, p. 308-314, 2014.
10.1016/j.foodchem.2014.01.010
url https://www.arca.fiocruz.br/handle/icict/13586
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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