STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS

Detalhes bibliográficos
Autor(a) principal: Carvalho,B. M. A.
Data de Publicação: 2016
Outros Autores: Silva Júnior,W. F., Carvalho,L. M., Minim,L. A., Carvalho,G. G. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322016000100215
Resumo: Abstract Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.
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spelling STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONSSMAIMACCryogelProteinAbstract Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.Brazilian Society of Chemical Engineering2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322016000100215Brazilian Journal of Chemical Engineering v.33 n.1 2016reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20160331s20140112info:eu-repo/semantics/openAccessCarvalho,B. M. A.Silva Júnior,W. F.Carvalho,L. M.Minim,L. A.Carvalho,G. G. P.eng2016-07-06T00:00:00Zoai:scielo:S0104-66322016000100215Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2016-07-06T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
title STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
spellingShingle STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
Carvalho,B. M. A.
SMA
IMAC
Cryogel
Protein
title_short STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
title_full STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
title_fullStr STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
title_full_unstemmed STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
title_sort STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
author Carvalho,B. M. A.
author_facet Carvalho,B. M. A.
Silva Júnior,W. F.
Carvalho,L. M.
Minim,L. A.
Carvalho,G. G. P.
author_role author
author2 Silva Júnior,W. F.
Carvalho,L. M.
Minim,L. A.
Carvalho,G. G. P.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho,B. M. A.
Silva Júnior,W. F.
Carvalho,L. M.
Minim,L. A.
Carvalho,G. G. P.
dc.subject.por.fl_str_mv SMA
IMAC
Cryogel
Protein
topic SMA
IMAC
Cryogel
Protein
description Abstract Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322016000100215
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322016000100215
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0104-6632.20160331s20140112
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.33 n.1 2016
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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