High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients

Gomes, Shayane Martins Rodrigues; Brito, Andréia Carolinne de Souza; Manfro, Wânia Ferraz Pereira; Ribeiro-Alves, Marcelo; Ribeiro, Roberto Stefan de Almeida; Cal, Mariana Soares da; Lisboa, Vinicius da Cunha; Abreu, Daniel Paiva Barros de; Castilho, Leda dos Reis; Porto, Luís Cristóvão de Moares Sobrino; Mafort, Thiago Thomáz; Lopes, Agnaldo José; Silva, Silvia Amaral Gonçalves da; Dutra, Patrícia Maria Lourenço; Rodrigues, Luciana Silva

High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients

Detalhes bibliográficos
Autor(a) principal:	Gomes, Shayane Martins Rodrigues
Data de Publicação:	2023
Outros Autores:	Brito, Andréia Carolinne de Souza, Manfro, Wânia Ferraz Pereira, Ribeiro-Alves, Marcelo, Ribeiro, Roberto Stefan de Almeida, Cal, Mariana Soares da, Lisboa, Vinicius da Cunha, Abreu, Daniel Paiva Barros de, Castilho, Leda dos Reis, Porto, Luís Cristóvão de Moares Sobrino, Mafort, Thiago Thomáz, Lopes, Agnaldo José, Silva, Silvia Amaral Gonçalves da, Dutra, Patrícia Maria Lourenço, Rodrigues, Luciana Silva
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/58995
Resumo:	This work was supported by the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), award number E-26/211.155/2019. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Metadados do item

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spelling	Gomes, Shayane Martins RodriguesBrito, Andréia Carolinne de SouzaManfro, Wânia Ferraz PereiraRibeiro-Alves, MarceloRibeiro, Roberto Stefan de AlmeidaCal, Mariana Soares daLisboa, Vinicius da CunhaAbreu, Daniel Paiva Barros deCastilho, Leda dos ReisPorto, Luís Cristóvão de Moares SobrinoMafort, Thiago ThomázLopes, Agnaldo JoséSilva, Silvia Amaral Gonçalves daDutra, Patrícia Maria LourençoRodrigues, Luciana Silva2023-06-13T01:03:07Z2023-06-13T01:03:07Z2023GOMES, Shayane Martins Rodrigues et al. High levels of pro-inflammatory SARS-CoV-2-specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients. PLoS One, v. 18, n. 4, p. 1-20, Apr. 2023.1932-6203https://www.arca.fiocruz.br/handle/icict/5899510.1371/journal.pone.02839831932-6203This work was supported by the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), award number E-26/211.155/2019. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil.Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil.Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil.Oswaldo Cruz Foundation. National Institute of Infectology Evandro Chagas. Laboratory of Clinical Research on STD/AID. Rio de Janeiro, RJ, Brazil.Rio de Janeiro State University. Department of General Pathology and Laboratories. Laboratory of Immunopathology. Rio de Janeiro, RJ, Brazil.Pedro Ernesto University Hospital. Policlínica Piquet Carneiro. Pulmonary and Tisiology Department. Rio de Janeiro, RJ, Brazil.Rio de Janeiro State University. Department of General Pathology and Laboratories. Laboratory of Immunopathology. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Chemical Engineering Program, Cell Culture Engineering Lab (COPPE). Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Chemical Engineering Program, Cell Culture Engineering Lab (COPPE). Rio de Janeiro, RJ, Brazil.Rio de Janeiro State University. Department of Histology and Embryology Institute of Biology Roberto Alcantara Gomes. Histocompatibility and Cryopreservation Laboratory. Rio de Janeiro, RJ, Brazil.Pedro Ernesto University Hospital. Policlínica Piquet Carneiro. Pulmonary and Tisiology Department. Rio de Janeiro, RJ, Brazil.Pedro Ernesto University Hospital. Policlínica Piquet Carneiro. Pulmonary and Tisiology Department. Rio de Janeiro, RJ, Brazil.Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil.Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil.Rio de Janeiro State University. Department of General Pathology and Laboratories. Laboratory of Immunopathology. Rio de Janeiro, RJ, Brazil.Background: Cytokines induced by SARS-CoV-2 infection play a crucial role in the pathophysiology of COVID-19 and hyperinflammatory responses have been associated with poor clinical outcomes, with progression to severe conditions or long-term subacute complications named as long-COVID-19. Methods: In this cross-sectional study, we aimed to evaluate a set of antigen-specific inflammatory cytokines in blood from recovered COVID-19 individuals or who suffered a post-acute phase of SARS-CoV-2 infection compared to healthy individuals with no history of COVID-19 exposition or infection. Interferon-gamma (IFN-γ), IFN-γ-induced protein 10 (IP-10), tumor necrosis factor (TNF), IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, and IL-17A were quantified by multiplex cytometric bead assay and enzyme-linked immunosorbent assay after stimulation of whole blood with recombinant Spike protein from SARS-CoV-2. Additionally, all participants have evaluated for anti-(S) protein-specific IgG antibodies. Clinical specimens were collected within two months of COVID-19 diagnosis. Results: A total of 47 individuals were enrolled in the study, a median age of 43 years (IQR = 14.5), grouped into healthy individuals with no history of infection or exposure to SARS-CoV-2 (unexposed group; N = 21); and patients from the Health Complex of the Rio de Janeiro State University (UERJ), Brazil, who were SARS-CoV-2 positive by RT-PCR (COVID-19 group)-categorized as recovered COVID-19 (N = 11) or long-COVID-19 (N = 15). All COVID-19 patients presented at least one signal or symptom during the first two weeks of infection. Six patients were hospitalized and required invasive mechanical ventilation. Our results showed that COVID-19 patients had significantly higher levels of IFN-γ, TNF, IL-1β, IL-2, IL-6, IL-8, and IP-10 than the unexposed group. The long-COVID-19 group has presented significantly high levels of IL-1β and IL-6 compared to unexposed individuals, but not from recovered COVID-19. A principal-component analysis demonstrated 84.3% of the total variance of inflammatory-SARS-CoV-2 response in the first two components, and it was possible to stratify IL-6, TNF, IL-1β, IL-10, and IL-2 as the top-five cytokines which are candidates to discriminate COVID-19 group (including long-COVID-19 subgroup) and healthy unexposed individuals. Conclusion: We revealed important S protein-specific differential biomarkers in individuals affected by COVID-19, bringing new insights into the inflammatory status or SARS-CoV-2 exposition determination.engPublic Library of ScienceHigh levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSARS-CoV-2COVID-19Cytokine Release Assay (CRA)Long-COVID-19 patientsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv	High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
title	High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
spellingShingle	High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients Gomes, Shayane Martins Rodrigues SARS-CoV-2 COVID-19 Cytokine Release Assay (CRA) Long-COVID-19 patients
title_short	High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
title_full	High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
title_fullStr	High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
title_full_unstemmed	High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
title_sort	High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
author	Gomes, Shayane Martins Rodrigues
author_facet	Gomes, Shayane Martins Rodrigues Brito, Andréia Carolinne de Souza Manfro, Wânia Ferraz Pereira Ribeiro-Alves, Marcelo Ribeiro, Roberto Stefan de Almeida Cal, Mariana Soares da Lisboa, Vinicius da Cunha Abreu, Daniel Paiva Barros de Castilho, Leda dos Reis Porto, Luís Cristóvão de Moares Sobrino Mafort, Thiago Thomáz Lopes, Agnaldo José Silva, Silvia Amaral Gonçalves da Dutra, Patrícia Maria Lourenço Rodrigues, Luciana Silva
author_role	author
author2	Brito, Andréia Carolinne de Souza Manfro, Wânia Ferraz Pereira Ribeiro-Alves, Marcelo Ribeiro, Roberto Stefan de Almeida Cal, Mariana Soares da Lisboa, Vinicius da Cunha Abreu, Daniel Paiva Barros de Castilho, Leda dos Reis Porto, Luís Cristóvão de Moares Sobrino Mafort, Thiago Thomáz Lopes, Agnaldo José Silva, Silvia Amaral Gonçalves da Dutra, Patrícia Maria Lourenço Rodrigues, Luciana Silva
author2_role	author author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Gomes, Shayane Martins Rodrigues Brito, Andréia Carolinne de Souza Manfro, Wânia Ferraz Pereira Ribeiro-Alves, Marcelo Ribeiro, Roberto Stefan de Almeida Cal, Mariana Soares da Lisboa, Vinicius da Cunha Abreu, Daniel Paiva Barros de Castilho, Leda dos Reis Porto, Luís Cristóvão de Moares Sobrino Mafort, Thiago Thomáz Lopes, Agnaldo José Silva, Silvia Amaral Gonçalves da Dutra, Patrícia Maria Lourenço Rodrigues, Luciana Silva
dc.subject.en.en_US.fl_str_mv	SARS-CoV-2 COVID-19 Cytokine Release Assay (CRA) Long-COVID-19 patients
topic	SARS-CoV-2 COVID-19 Cytokine Release Assay (CRA) Long-COVID-19 patients
description	This work was supported by the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), award number E-26/211.155/2019. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
publishDate	2023
dc.date.accessioned.fl_str_mv	2023-06-13T01:03:07Z
dc.date.available.fl_str_mv	2023-06-13T01:03:07Z
dc.date.issued.fl_str_mv	2023
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	GOMES, Shayane Martins Rodrigues et al. High levels of pro-inflammatory SARS-CoV-2-specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients. PLoS One, v. 18, n. 4, p. 1-20, Apr. 2023.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/58995
dc.identifier.issn.en_US.fl_str_mv	1932-6203
dc.identifier.doi.none.fl_str_mv	10.1371/journal.pone.0283983
dc.identifier.eissn.none.fl_str_mv	1932-6203
identifier_str_mv	GOMES, Shayane Martins Rodrigues et al. High levels of pro-inflammatory SARS-CoV-2-specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients. PLoS One, v. 18, n. 4, p. 1-20, Apr. 2023. 1932-6203 10.1371/journal.pone.0283983
url	https://www.arca.fiocruz.br/handle/icict/58995
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Public Library of Science
publisher.none.fl_str_mv	Public Library of Science
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/58995/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/58995/2/ve_Shayane_Gomes_etal_INI_2023.pdf
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients

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