Serological Screening of the Schistosoma mansoni Adult Worm Proteome
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/9415 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Biologia e Genômica Computacional. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil. |
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Ludolf, Fernanda RibeiroPatrocinio, Paola RezendeOliveira, Rodrigo CorrêaOliveira, Andréa Gazzinelli Corrêa deFalcone, Franco H.Ferreira, Andre TeixeiraPerales, JonasOliveira, Guilherme CorrêaPereira, Rosiane Aparecida da Silva2015-02-03T12:11:28Z2015-02-03T12:11:28Z2014LUDOLF, Fernanda Ribeiro et al. Serological Screening of the Schistosoma mansoni Adult Worm Proteome. PLoS Neglected Tropical Diseases, v. 8, n. 3, p. 1-16, 2014.1935-2735https://www.arca.fiocruz.br/handle/icict/941510.1371/journal.pntd.0002745engPublic Library of ScienceSerological Screening of the Schistosoma mansoni Adult Worm Proteomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Biologia e Genômica Computacional. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Biologia e Genômica Computacional. Belo Horizonte, MG, Brasil.Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil / Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brasil.The University of Nottingham. School of Pharmacy. Division of Molecular and Cellular Science. Nottingham, East Midlands, United Kingdom.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Toxicologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmodinâmica. Laboratório de Toxicologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Biologia e Genômica Computacional. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Biologia e Genômica Computacional. Belo Horizonte, MG, Brasil.BACKGROUND: New interventions tools are a priority for schistosomiasis control and elimination, as the disease is still highly prevalent. The identification of proteins associated with active infection and protective immune response may constitute the basis for the development of a successful vaccine and could also indicate new diagnostic candidates. In this context, post-genomic technologies have been progressing, resulting in a more rational discovery of new biomarkers of resistance and antigens for diagnosis. METHODOLOGY/PRINCIPAL FINDINGS: Two-dimensional electrophoresed Schistosoma mansoni adult worm protein extracts were probed with pooled sera of infected and non-infected (naturally resistant) individuals from a S. mansoni endemic area. A total of 47 different immunoreactive proteins were identified by mass spectrometry. Although the different pooled sera shared most of the immunoreactive protein spots, nine protein spots reacted exclusively with the serum pool of infected individuals, which correspond to annexin, major egg antigen, troponin T, filamin, disulphide-isomerase ER-60 precursor, actin and reticulocalbin. One protein spot, corresponding to eukaryotic translation elongation factor, reacted exclusively with the pooled sera of non-infected individuals living in the endemic area. Western blotting of two selected recombinant proteins, major egg antigen and hemoglobinase, showed a similar recognition pattern of that of the native protein. CONCLUDING/SIGNIFICANCE: Using a serological proteome analysis, a group of antigens related to the different infection status of the endemic area residents was identified and may be related to susceptibility or resistance to infection.AntibodiesAntigensElectrophoresisProteomeSchistosoma mansoniHelminthImmunologyTwo-Dimensionalinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9415/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_028.pdf2014_028.pdfapplication/pdf5119906https://www.arca.fiocruz.br/bitstream/icict/9415/2/2014_028.pdf8854d1cf236c709d1f6750e605a352a4MD52TEXT2014_028.pdf.txt2014_028.pdf.txtExtracted texttext/plain90159https://www.arca.fiocruz.br/bitstream/icict/9415/3/2014_028.pdf.txt6320f336f6e9632daf48510664bb74c0MD53icict/94152023-09-05 10:30:52.595oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:30:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Serological Screening of the Schistosoma mansoni Adult Worm Proteome |
| title |
Serological Screening of the Schistosoma mansoni Adult Worm Proteome |
| spellingShingle |
Serological Screening of the Schistosoma mansoni Adult Worm Proteome Ludolf, Fernanda Ribeiro Antibodies Antigens Electrophoresis Proteome Schistosoma mansoni Helminth Immunology Two-Dimensional |
| title_short |
Serological Screening of the Schistosoma mansoni Adult Worm Proteome |
| title_full |
Serological Screening of the Schistosoma mansoni Adult Worm Proteome |
| title_fullStr |
Serological Screening of the Schistosoma mansoni Adult Worm Proteome |
| title_full_unstemmed |
Serological Screening of the Schistosoma mansoni Adult Worm Proteome |
| title_sort |
Serological Screening of the Schistosoma mansoni Adult Worm Proteome |
| author |
Ludolf, Fernanda Ribeiro |
| author_facet |
Ludolf, Fernanda Ribeiro Patrocinio, Paola Rezende Oliveira, Rodrigo Corrêa Oliveira, Andréa Gazzinelli Corrêa de Falcone, Franco H. Ferreira, Andre Teixeira Perales, Jonas Oliveira, Guilherme Corrêa Pereira, Rosiane Aparecida da Silva |
| author_role |
author |
| author2 |
Patrocinio, Paola Rezende Oliveira, Rodrigo Corrêa Oliveira, Andréa Gazzinelli Corrêa de Falcone, Franco H. Ferreira, Andre Teixeira Perales, Jonas Oliveira, Guilherme Corrêa Pereira, Rosiane Aparecida da Silva |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ludolf, Fernanda Ribeiro Patrocinio, Paola Rezende Oliveira, Rodrigo Corrêa Oliveira, Andréa Gazzinelli Corrêa de Falcone, Franco H. Ferreira, Andre Teixeira Perales, Jonas Oliveira, Guilherme Corrêa Pereira, Rosiane Aparecida da Silva |
| dc.subject.en.pt_BR.fl_str_mv |
Antibodies Antigens Electrophoresis Proteome Schistosoma mansoni Helminth Immunology Two-Dimensional |
| topic |
Antibodies Antigens Electrophoresis Proteome Schistosoma mansoni Helminth Immunology Two-Dimensional |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Grupo de Biologia e Genômica Computacional. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil. |
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2014 |
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2014 |
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2015-02-03T12:11:28Z |
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2015-02-03T12:11:28Z |
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info:eu-repo/semantics/article |
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LUDOLF, Fernanda Ribeiro et al. Serological Screening of the Schistosoma mansoni Adult Worm Proteome. PLoS Neglected Tropical Diseases, v. 8, n. 3, p. 1-16, 2014. |
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10.1371/journal.pntd.0002745 |
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LUDOLF, Fernanda Ribeiro et al. Serological Screening of the Schistosoma mansoni Adult Worm Proteome. PLoS Neglected Tropical Diseases, v. 8, n. 3, p. 1-16, 2014. 1935-2735 10.1371/journal.pntd.0002745 |
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