LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)

Detalhes bibliográficos
Autor(a) principal: Bonyek-Silva, Icaro
Data de Publicação: 2021
Outros Autores: Machado, Antônio Fernando Araujo, Silva, Thiago Cerqueira, Nunes, Sara, Cruz, Marcio Rivison Silva, Silva, Jessica, Santos, Reinan Lima, Barral, Aldina Maria do Prado, Oliveira, Pablo Rafael Silveira, Khouri, Antonio Ricardo, Serezani, C. Henrique, Brodskyn, Claudia Ida, Caldas, Juliana Ribeiro, Manoel, Barral Netto, Boaventura, Viviane, Tavares, Natalia Machado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50680
Resumo: Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil.
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spelling Bonyek-Silva, IcaroMachado, Antônio Fernando AraujoSilva, Thiago CerqueiraNunes, SaraCruz, Marcio Rivison SilvaSilva, JessicaSantos, Reinan LimaBarral, Aldina Maria do PradoOliveira, Pablo Rafael SilveiraKhouri, Antonio RicardoSerezani, C. HenriqueBrodskyn, Claudia IdaCaldas, Juliana RibeiroManoel, Barral NettoBoaventura, VivianeTavares, Natalia Machado2022-01-07T21:27:42Z2022-01-07T21:27:42Z2021BONYEK-SILVA, Icaro et al. LTB4-Driven Inflammation and Increased Expression of ALOX5/ACE2 During Severe COVID-19 in Individuals With Diabetes. Diabetes, p. 1-47, 15 Jun. 2021.0012-1797https://www.arca.fiocruz.br/handle/icict/50680engAmerican Diabetes Associationhttps://www.arca.fiocruz.br/handle/icict/49900COVID-19DiabetesSARS-CoV-2LTB4ACE2COVID-19DiabetesLipid mediatorsLTB4ACE2SARS-CoV-2LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil.Salvador University. Salvador, BA, Brazil.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil.Salvador University. Salvador, BA, Brazil / Ernesto Simões Filho Hospital. Critical Care Unit. Salvador, BA, Brazil. Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Pharmacy School. Salvador, BA, Brazil.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil / National Institute of Science and Technology. Institute of Investigation in Immunology. São Paulo, SP, Brazil.Federal University of Bahia. Institute of Biological Sciences. Salvador, BA, Brazil.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil.Vanderbilt University. Department of Medicine. Division of Infectious Diseases. Medical Center. Nashville, TN, USA.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil / National Institute of Science and Technology. Institute of Investigation in Immunology. São Paulo, SP, Brazil.Salvador University. Salvador, BA, Brazil / Ernesto Simões Filho Hospital. Critical Care Unit. Salvador, BA, Brazil / São Rafael Hospital–Rede d’Or. Critical Care Unit. Salvador, BA, Brazil / Bahiana School of Medicine and Public Health. Salvador, BA, Brazil.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil / National Institute of Science and Technology. Institute of Investigation in Immunology. São Paulo, SP, Brazil.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil.Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil / National Institute of Science and Technology. Institute of Investigation in Immunology. São Paulo, SP, Brazil.Diabetes is a known risk factor for severe COVID-19, the disease caused by the new coronavirus SARS-CoV-2. However, there is a lack of knowledge about the mechanisms involved in the evolution of COVID-19 in individuals with diabetes. Therefore, we aimed to evaluate whether the chronic low-grade inflammation of diabetes could play a role in the development of severe COVID-19. We collected clinical data and blood samples of hospitalized patients for COVID-19, with diabetes and without diabetes. Plasma samples were used to measure inflammatory mediators and peripheral blood mononuclear cells, for gene expression analysis of SARS-CoV-2 main receptor system (ACE2/TMPRSS2) and main molecule of LTB4 pathway (ALOX5). We found that diabetes activates LTB4 pathway, and during COVID-19, it increases ACE2/TMPRSS2 as well as ALOX5 expression. Diabetes was also associated with COVID-19-related disorders, such as reduced SpO2/FiO2 and PaO2/FiO2 levels, and increased disease duration. In addition, the expression of ACE2 and ALOX5 are positively correlated, with increased expression in COVID-19 patients with diabetes requiring intensive care assistance. We confirmed these molecular results at the protein level, where plasma LTB4 is significantly increased in individuals with diabetes. Besides, IL-6 serum levels are increased only in individuals with diabetes requiring intensive care assistance. Together, these results indicate that LTB4 and IL-6 systemic levels, as well as, ACE2/ALOX5 blood expression could be early markers of severe COVID-19 in individuals with diabetes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83134https://www.arca.fiocruz.br/bitstream/icict/50680/1/license.txt0ab46789d568c4ba27c7b5d29a9fe9c4MD51ORIGINALBonyek-Silva_Icaro_etal_IGM_COVID-19_2021.pdfBonyek-Silva_Icaro_etal_IGM_COVID-19_2021.pdfapplication/pdf2662004https://www.arca.fiocruz.br/bitstream/icict/50680/2/Bonyek-Silva_Icaro_etal_IGM_COVID-19_2021.pdf24a199f0edbb704297c76b0cee02af90MD52icict/506802022-01-07 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dc.title.pt_BR.fl_str_mv LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)
title LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)
spellingShingle LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)
Bonyek-Silva, Icaro
COVID-19
Diabetes
SARS-CoV-2
LTB4
ACE2
COVID-19
Diabetes
Lipid mediators
LTB4
ACE2
SARS-CoV-2
title_short LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)
title_full LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)
title_fullStr LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)
title_full_unstemmed LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)
title_sort LTB4-driven inflammation and increased expression of ALOX5/ACE2 during severe COVID-19 in individuals with diabetes (Preprint)
author Bonyek-Silva, Icaro
author_facet Bonyek-Silva, Icaro
Machado, Antônio Fernando Araujo
Silva, Thiago Cerqueira
Nunes, Sara
Cruz, Marcio Rivison Silva
Silva, Jessica
Santos, Reinan Lima
Barral, Aldina Maria do Prado
Oliveira, Pablo Rafael Silveira
Khouri, Antonio Ricardo
Serezani, C. Henrique
Brodskyn, Claudia Ida
Caldas, Juliana Ribeiro
Manoel, Barral Netto
Boaventura, Viviane
Tavares, Natalia Machado
author_role author
author2 Machado, Antônio Fernando Araujo
Silva, Thiago Cerqueira
Nunes, Sara
Cruz, Marcio Rivison Silva
Silva, Jessica
Santos, Reinan Lima
Barral, Aldina Maria do Prado
Oliveira, Pablo Rafael Silveira
Khouri, Antonio Ricardo
Serezani, C. Henrique
Brodskyn, Claudia Ida
Caldas, Juliana Ribeiro
Manoel, Barral Netto
Boaventura, Viviane
Tavares, Natalia Machado
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bonyek-Silva, Icaro
Machado, Antônio Fernando Araujo
Silva, Thiago Cerqueira
Nunes, Sara
Cruz, Marcio Rivison Silva
Silva, Jessica
Santos, Reinan Lima
Barral, Aldina Maria do Prado
Oliveira, Pablo Rafael Silveira
Khouri, Antonio Ricardo
Serezani, C. Henrique
Brodskyn, Claudia Ida
Caldas, Juliana Ribeiro
Manoel, Barral Netto
Boaventura, Viviane
Tavares, Natalia Machado
dc.subject.other.pt_BR.fl_str_mv COVID-19
Diabetes
SARS-CoV-2
LTB4
ACE2
topic COVID-19
Diabetes
SARS-CoV-2
LTB4
ACE2
COVID-19
Diabetes
Lipid mediators
LTB4
ACE2
SARS-CoV-2
dc.subject.en.pt_BR.fl_str_mv COVID-19
Diabetes
Lipid mediators
LTB4
ACE2
SARS-CoV-2
description Oswaldo Cruz Foundation. Gonçalo Moniz Institute. Salvador, BA, Brazil / Federal University of Bahia. Medical School. Salvador, BA, Brazil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-07T21:27:42Z
dc.date.available.fl_str_mv 2022-01-07T21:27:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BONYEK-SILVA, Icaro et al. LTB4-Driven Inflammation and Increased Expression of ALOX5/ACE2 During Severe COVID-19 in Individuals With Diabetes. Diabetes, p. 1-47, 15 Jun. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50680
dc.identifier.issn.pt_BR.fl_str_mv 0012-1797
identifier_str_mv BONYEK-SILVA, Icaro et al. LTB4-Driven Inflammation and Increased Expression of ALOX5/ACE2 During Severe COVID-19 in Individuals With Diabetes. Diabetes, p. 1-47, 15 Jun. 2021.
0012-1797
url https://www.arca.fiocruz.br/handle/icict/50680
dc.language.iso.fl_str_mv eng
language eng
dc.relation.hasversion.pt_BR.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49900
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Diabetes Association
publisher.none.fl_str_mv American Diabetes Association
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50680/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50680/2/Bonyek-Silva_Icaro_etal_IGM_COVID-19_2021.pdf
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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