Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes

Detalhes bibliográficos
Autor(a) principal: Diniz, Suelen Queiroz
Data de Publicação: 2021
Outros Autores: Carvalho, Andréa Teixeira, Figueiredo, Maria Marta, Costa, Pedro Augusto Carvalho, Rocha, Bruno Coelho, Martins Filho, Olindo Assis, Gonçalves, Ricardo, Pereira, Dhélio Batista, Tada, Mauro Shugiro, Oliveira, Fabiano, Gazzinelli, Ricardo Tostes, Antonelli, Lis Ribeiro do Valle
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51772
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.
id CRUZ_b2fd5442c244452aad25e5445053fdf9
oai_identifier_str oai:www.arca.fiocruz.br:icict/51772
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Diniz, Suelen QueirozCarvalho, Andréa TeixeiraFigueiredo, Maria MartaCosta, Pedro Augusto CarvalhoRocha, Bruno CoelhoMartins Filho, Olindo AssisGonçalves, RicardoPereira, Dhélio BatistaTada, Mauro ShugiroOliveira, FabianoGazzinelli, Ricardo TostesAntonelli, Lis Ribeiro do Valle2022-03-21T16:03:53Z2022-03-21T16:03:53Z2021DINIZ, Suelen Queiroz et al. Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes. mBio, v. 12, n. 4, e0124721 , 2021. doi: 10.1128/mBio.01247-21.2150-7511https://www.arca.fiocruz.br/handle/icict/5177210.1128/mBio.01247-21engAmerican Society for MicrobiologyPlasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Patologia Geral. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brazil.Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brazil.National Institutes of Healthgrid. Laboratory of Malaria and Vector Research. Rockville, Maryland, USAFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Monocytes play an important role in the host defense against Plasmodium vivax as the main source of inflammatory cytokines and mitochondrial reactive oxygen species (mROS). Here, we show that monocyte metabolism is altered during human P. vivax malaria, with mitochondria playing a major function in this switch. The process involves a reprograming in which the cells increase glucose uptake and produce ATP via glycolysis instead of oxidative phosphorylation. P. vivax infection results in dysregulated mitochondrial gene expression and in altered membrane potential leading to mROS increase rather than ATP production. When monocytes were incubated with P. vivax-infected reticulocytes, mitochondria colocalized with phagolysosomes containing parasites representing an important source mROS. Importantly, the mitochondrial enzyme superoxide dismutase 2 (SOD2) is simultaneously induced in monocytes from malaria patients. Taken together, the monocyte metabolic reprograming with an increased mROS production may contribute to protective responses against P. vivax while triggering immunomodulatory mechanisms to circumvent tissue damage. IMPORTANCE Plasmodium vivax is the most widely distributed causative agent of human malaria. To achieve parasite control, the human immune system develops a substantial inflammatory response that is also responsible for the symptoms of the disease. Among the cells involved in this response, monocytes play an important role. Here, we show that monocyte metabolism is altered during malaria, with its mitochondria playing a major function in this switch. This change involves a reprograming process in which the cells increase glucose uptake and produce ATP via glycolysis instead of oxidative phosphorylation. The resulting altered mitochondrial membrane potential leads to an increase in mitochondrial reactive oxygen species rather than ATP. These data suggest that agents that change metabolism should be investigated and used with caution during malaria.P. vivaxmalariametabolismmitochondriamitochondrial metabolismmonocytesreactive oxygen speciesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51772/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALPlasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes.pdfPlasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes.pdfapplication/pdf5295117https://www.arca.fiocruz.br/bitstream/icict/51772/2/Plasmodium%20vivax%20Infection%20Alters%20Mitochondrial%20Metabolism%20in%20Human%20Monocytes.pdf94da562d49855fd0a8340335f1ddba28MD52icict/517722022-03-21 13:03:53.768oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-03-21T16:03:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes
title Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes
spellingShingle Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes
Diniz, Suelen Queiroz
P. vivax
malaria
metabolism
mitochondria
mitochondrial metabolism
monocytes
reactive oxygen species
title_short Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes
title_full Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes
title_fullStr Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes
title_full_unstemmed Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes
title_sort Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes
author Diniz, Suelen Queiroz
author_facet Diniz, Suelen Queiroz
Carvalho, Andréa Teixeira
Figueiredo, Maria Marta
Costa, Pedro Augusto Carvalho
Rocha, Bruno Coelho
Martins Filho, Olindo Assis
Gonçalves, Ricardo
Pereira, Dhélio Batista
Tada, Mauro Shugiro
Oliveira, Fabiano
Gazzinelli, Ricardo Tostes
Antonelli, Lis Ribeiro do Valle
author_role author
author2 Carvalho, Andréa Teixeira
Figueiredo, Maria Marta
Costa, Pedro Augusto Carvalho
Rocha, Bruno Coelho
Martins Filho, Olindo Assis
Gonçalves, Ricardo
Pereira, Dhélio Batista
Tada, Mauro Shugiro
Oliveira, Fabiano
Gazzinelli, Ricardo Tostes
Antonelli, Lis Ribeiro do Valle
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Diniz, Suelen Queiroz
Carvalho, Andréa Teixeira
Figueiredo, Maria Marta
Costa, Pedro Augusto Carvalho
Rocha, Bruno Coelho
Martins Filho, Olindo Assis
Gonçalves, Ricardo
Pereira, Dhélio Batista
Tada, Mauro Shugiro
Oliveira, Fabiano
Gazzinelli, Ricardo Tostes
Antonelli, Lis Ribeiro do Valle
dc.subject.en.pt_BR.fl_str_mv P. vivax
malaria
metabolism
mitochondria
mitochondrial metabolism
monocytes
reactive oxygen species
topic P. vivax
malaria
metabolism
mitochondria
mitochondrial metabolism
monocytes
reactive oxygen species
description Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-03-21T16:03:53Z
dc.date.available.fl_str_mv 2022-03-21T16:03:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DINIZ, Suelen Queiroz et al. Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes. mBio, v. 12, n. 4, e0124721 , 2021. doi: 10.1128/mBio.01247-21.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51772
dc.identifier.issn.pt_BR.fl_str_mv 2150-7511
dc.identifier.doi.none.fl_str_mv 10.1128/mBio.01247-21
identifier_str_mv DINIZ, Suelen Queiroz et al. Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes. mBio, v. 12, n. 4, e0124721 , 2021. doi: 10.1128/mBio.01247-21.
2150-7511
10.1128/mBio.01247-21
url https://www.arca.fiocruz.br/handle/icict/51772
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51772/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51772/2/Plasmodium%20vivax%20Infection%20Alters%20Mitochondrial%20Metabolism%20in%20Human%20Monocytes.pdf
bitstream.checksum.fl_str_mv 9193a7c197bc67acd023525e72a03240
94da562d49855fd0a8340335f1ddba28
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009212153790464

Registros relacionados