Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease.
| Autor(a) principal: | |
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| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26785 |
Resumo: | 2020-01-01 |
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Medeiros, Nayara Ingrid deGusmão, Rafaelle Christine Gomes FaresFranco, Eliza P.Sousa, Giovane R.Mattos, Rafael Teixeira deChaves, Ana TherezaNunes, Maria do Carmo PereiraDutra, Walderez OrnelasOliveira, Rodrigo Correa deRocha, Manoel Otávio da CostaEstanislau, Juliana de Assis Silva Gomes2018-06-07T18:14:28Z2018-06-07T18:14:28Z2017MEDEIROS, Nayara Ingrid de et al. Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. PLoS Negl Trop Dis., v. 11, n. 1, e0005284, 2017.1935-2727https://www.arca.fiocruz.br/handle/icict/2678510.1371/journal.pntd.0005284engPublic Library of ScienceDoença de ChagasDoenças cardiacasChagas diseasecardiac diseasesDifferential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, BrasilUnited States Food and Drug Administration. Center for Biologics Evaluation and Research - Silver Spring. Maryland, United States of America/Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil/Instituto Nacional de Ciência e Tecnologia em Doenças Topicais. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Instituto Nacional de Ciência e Tecnologia em Doenças Topicais. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, BrasilDilated cardiomyopathy, the most severe manifestation in chronic phase of Chagas disease, affects about 30% of patients and is characterized by myocardial dysfunction and interstitial fibrosis due to extracellular matrix (ECM) remodeling. ECM remodeling is regulated by proteolytic enzymes such as matrix metalloproteinases (MMPs) and cytokines produced by immune cells, including phagocytes. We evaluated by flow cytometry the expression of MMP-2, MMP-9, IL-1β, TNF-α, TGF-β and IL-10 by neutrophils and monocytes from patients with indeterminate (IND) and cardiac (CARD) clinical forms of Chagas disease and non-infected individuals (NI), before and after in vitro stimulation with Trypanosoma cruzi antigens. Our results showed an important contribution of neutrophils for MMPs production, while monocytes seemed to be involved in cytokine production. The results showed that neutrophils and monocytes from IND and CARD patients had higher intracellular levels of MMP-2 and MMP-9 than NI individuals. On the other hand, T. cruzi derived-antigens promote a differential expression of MMP-2 and MMP-9 in patients with Chagas disease and may regulate MMPs expression in neutrophils and monocytes, mainly when a cardiac alteration is not present. Our data also showed that in the presence of T. cruzi derived-antigens the production of cytokines by neutrophils and monocytes, but mainly by monocytes, may be intensified. Correlation analysis demonstrated that MMP-2 had a positive correlation with IL-10 and a negative correlation with IL-1β, whereas MMP-9 showed a negative correlation with IL-10. We also observed that IND patients presented a greater percentage of high producer cells of regulatory molecules when compared to CARD patients, indicating a different pattern in the immune response. Our data suggest that MMPs and cytokines produced by neutrophils and monocytes are important contributors for cardiac remodeling and may be an interesting target for new biomarker research.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/26785/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALDifferential Expression of Matrix Metalloproteinases.pdfDifferential Expression of Matrix Metalloproteinases.pdfapplication/pdf1619739https://www.arca.fiocruz.br/bitstream/icict/26785/2/Differential%20Expression%20of%20Matrix%20Metalloproteinases.pdf351c68a912f737a0e9c1e317a65b5a37MD52TEXTDifferential Expression of Matrix Metalloproteinases.pdf.txtDifferential Expression of Matrix Metalloproteinases.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. |
| title |
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. |
| spellingShingle |
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. Medeiros, Nayara Ingrid de Doença de Chagas Doenças cardiacas Chagas disease cardiac diseases |
| title_short |
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. |
| title_full |
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. |
| title_fullStr |
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. |
| title_full_unstemmed |
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. |
| title_sort |
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. |
| author |
Medeiros, Nayara Ingrid de |
| author_facet |
Medeiros, Nayara Ingrid de Gusmão, Rafaelle Christine Gomes Fares Franco, Eliza P. Sousa, Giovane R. Mattos, Rafael Teixeira de Chaves, Ana Thereza Nunes, Maria do Carmo Pereira Dutra, Walderez Ornelas Oliveira, Rodrigo Correa de Rocha, Manoel Otávio da Costa Estanislau, Juliana de Assis Silva Gomes |
| author_role |
author |
| author2 |
Gusmão, Rafaelle Christine Gomes Fares Franco, Eliza P. Sousa, Giovane R. Mattos, Rafael Teixeira de Chaves, Ana Thereza Nunes, Maria do Carmo Pereira Dutra, Walderez Ornelas Oliveira, Rodrigo Correa de Rocha, Manoel Otávio da Costa Estanislau, Juliana de Assis Silva Gomes |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Medeiros, Nayara Ingrid de Gusmão, Rafaelle Christine Gomes Fares Franco, Eliza P. Sousa, Giovane R. Mattos, Rafael Teixeira de Chaves, Ana Thereza Nunes, Maria do Carmo Pereira Dutra, Walderez Ornelas Oliveira, Rodrigo Correa de Rocha, Manoel Otávio da Costa Estanislau, Juliana de Assis Silva Gomes |
| dc.subject.other.pt_BR.fl_str_mv |
Doença de Chagas Doenças cardiacas |
| topic |
Doença de Chagas Doenças cardiacas Chagas disease cardiac diseases |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas disease cardiac diseases |
| description |
2020-01-01 |
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2017 |
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2017 |
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2018-06-07T18:14:28Z |
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2018-06-07T18:14:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MEDEIROS, Nayara Ingrid de et al. Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. PLoS Negl Trop Dis., v. 11, n. 1, e0005284, 2017. |
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https://www.arca.fiocruz.br/handle/icict/26785 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1935-2727 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0005284 |
| identifier_str_mv |
MEDEIROS, Nayara Ingrid de et al. Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. PLoS Negl Trop Dis., v. 11, n. 1, e0005284, 2017. 1935-2727 10.1371/journal.pntd.0005284 |
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Public Library of Science |
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Public Library of Science |
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