Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2

Detalhes bibliográficos
Autor(a) principal: Amorim, Natália R. T.
Data de Publicação: 2020
Outros Autores: Almeida, Glaucia Souza, Gomes, Tatiana Luna, Bozza, Patrícia T., Canetti, Claudio, Diaz, Bruno L., Maya-Monteiro, Clarissa M., Melo, Christianne Bandeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3389/fendo.2020.572113
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44799
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
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spelling Amorim, Natália R. T.Almeida, Glaucia SouzaGomes, Tatiana LunaBozza, Patrícia T.Canetti, ClaudioDiaz, Bruno L.Maya-Monteiro, Clarissa M.Melo, Christianne Bandeira2020-12-10T19:46:03Z2020-12-10T19:46:03Z2020AMORIM, Natália R. T. Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2. Frontiers in Endocrinology, v. 11, Article 572113, 12p, Sept. 2020.1664-2392https://www.arca.fiocruz.br/handle/icict/4479910.3389/fendo.2020.572113engFrontiers MediaLeptinaEosinófilos in vivoMigração e ativaçãoPGD2Derivado de mastócitosLeptinIn Vivo EosinophilMigration and ActivationPGD2Mast Cell-DerivedLeptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade de Campinas. Instituto de Biologia. Laboratório de Imunoinflamação. Campinas, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Instituto de Aplicação Fernando Rodrigues da Silveira. Departamento de Ciências da Natureza. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Eosinophils are key regulators of adipose tissue homeostasis, thus characterization of adipose tissue-related molecular factors capable of regulating eosinophil activity is of great interest. Leptin is known to directly activate eosinophils in vitro, but leptin ability of inducing in vivo eosinophilic inflammatory response remains elusive. Here, we show that leptin elicits eosinophil influx as well as its activation, characterized by increased lipid body biogenesis and LTC4 synthesis. Such leptin-triggered eosinophilic inflammatory response was shown to be dependent on activation of the mTOR signaling pathway, since it was (i) inhibited by rapamycin pre-treatment and (ii) reduced in PI3K-deficient mice. Local infiltration of activated eosinophils within leptin-driven inflammatory site was preceded by increased levels of classical mast cell-derived molecules, including TNFα, CCL5 (RANTES), and PGD2. Thus, mice were pre-treated with a mast cell degranulating agent compound 48/80 which was capable to impair leptin-induced PGD2 release, as well as eosinophil recruitment and activation. In agreement with an indirect mast cell-driven phenomenon, eosinophil accumulation induced by leptin was abolished in TNFR-1 deficient and also in HQL-79–pretreated mice, but not in mice pretreated with neutralizing antibodies against CCL5, indicating that both typical mast cell-driven signals TNFα and PGD2, but not CCL5, contribute to leptin-induced eosinophil influx. Distinctly, leptin-induced eosinophil lipid body (lipid droplet) assembly and LTC4 synthesis appears to depend on both PGD2 and CCL5, since both HQL-79 and anti-CCL5 treatments were able to inhibit these eosinophil activation markers. Altogether, our data show that leptin triggers eosinophilic inflammation in vivo via an indirect mechanism dependent on activation of resident mast cell secretory activity and mediation by TNFα, CCL5, and specially PGD2.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44799/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGlauciaSAlmeida_ClarissaMOnteiro_etal_IOC_2020.pdfGlauciaSAlmeida_ClarissaMOnteiro_etal_IOC_2020.pdfapplication/pdf1424195https://www.arca.fiocruz.br/bitstream/icict/44799/2/GlauciaSAlmeida_ClarissaMOnteiro_etal_IOC_2020.pdf2448b63edbd104a6a3b042459acc369aMD52TEXTGlauciaSAlmeida_ClarissaMOnteiro_etal_IOC_2020.pdf.txtGlauciaSAlmeida_ClarissaMOnteiro_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2
title Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2
spellingShingle Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2
Amorim, Natália R. T.
Leptina
Eosinófilos in vivo
Migração e ativação
PGD2
Derivado de mastócitos
Leptin
In Vivo Eosinophil
Migration and Activation
PGD2
Mast Cell-Derived
title_short Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2
title_full Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2
title_fullStr Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2
title_full_unstemmed Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2
title_sort Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2
author Amorim, Natália R. T.
author_facet Amorim, Natália R. T.
Almeida, Glaucia Souza
Gomes, Tatiana Luna
Bozza, Patrícia T.
Canetti, Claudio
Diaz, Bruno L.
Maya-Monteiro, Clarissa M.
Melo, Christianne Bandeira
author_role author
author2 Almeida, Glaucia Souza
Gomes, Tatiana Luna
Bozza, Patrícia T.
Canetti, Claudio
Diaz, Bruno L.
Maya-Monteiro, Clarissa M.
Melo, Christianne Bandeira
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Amorim, Natália R. T.
Almeida, Glaucia Souza
Gomes, Tatiana Luna
Bozza, Patrícia T.
Canetti, Claudio
Diaz, Bruno L.
Maya-Monteiro, Clarissa M.
Melo, Christianne Bandeira
dc.subject.other.pt_BR.fl_str_mv Leptina
Eosinófilos in vivo
Migração e ativação
PGD2
Derivado de mastócitos
topic Leptina
Eosinófilos in vivo
Migração e ativação
PGD2
Derivado de mastócitos
Leptin
In Vivo Eosinophil
Migration and Activation
PGD2
Mast Cell-Derived
dc.subject.en.pt_BR.fl_str_mv Leptin
In Vivo Eosinophil
Migration and Activation
PGD2
Mast Cell-Derived
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-12-10T19:46:03Z
dc.date.available.fl_str_mv 2020-12-10T19:46:03Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AMORIM, Natália R. T. Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2. Frontiers in Endocrinology, v. 11, Article 572113, 12p, Sept. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44799
dc.identifier.issn.pt_BR.fl_str_mv 1664-2392
dc.identifier.doi.none.fl_str_mv 10.3389/fendo.2020.572113
identifier_str_mv AMORIM, Natália R. T. Leptin Elicits In Vivo Eosinophil Migration and Activation: Key Role of Mast Cell-Derived PGD2. Frontiers in Endocrinology, v. 11, Article 572113, 12p, Sept. 2020.
1664-2392
10.3389/fendo.2020.572113
url https://www.arca.fiocruz.br/handle/icict/44799
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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