Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species

Detalhes bibliográficos
Autor(a) principal: Taverna, Constanza Giselle
Data de Publicação: 2013
Outros Autores: Borgeat, María Eugenia Bosco, Murisengo, Omar Alejandro, Davel, Graciela, Boité, Mariana Côrtes, Cupolillo, Elisa, Canteros, Cristina Elena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11781
Resumo: Instituto Nacional de Enfermedades Infecciosas Dr Carlos G Malbrán. Departamento Micología. Buenos Aires, Argentina.
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spelling Taverna, Constanza GiselleBorgeat, María Eugenia BoscoMurisengo, Omar AlejandroDavel, GracielaBoité, Mariana CôrtesCupolillo, ElisaCanteros, Cristina Elena2015-09-21T17:25:46Z2015-09-21T17:25:46Z2013TAVERNA, Constanza Giselle; et al. Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species. Mem Inst Oswaldo Cruz, v.108, n.2, p.178-185, April 2013.1678-8060https://www.arca.fiocruz.br/handle/icict/1178110.1590/0074-0276108022013009engFundação Oswaldo CruzComparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Enfermedades Infecciosas Dr Carlos G Malbrán. Departamento Micología. Buenos Aires, Argentina.Instituto Nacional de Enfermedades Infecciosas Dr Carlos G Malbrán. Departamento Micología. Buenos Aires, Argentina.Instituto Nacional de Enfermedades Infecciosas Dr Carlos G Malbrán. Departamento Micología. Buenos Aires, Argentina.Instituto Nacional de Enfermedades Infecciosas Dr Carlos G Malbrán. Departamento Micología. Buenos Aires, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Enfermedades Infecciosas Dr Carlos G Malbrán. Departamento Micología. Buenos Aires, Argentina.As the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means of accurately and rapidly identifying these species is necessary for the successful early resolution of infection and the subsequent reduction of morbidity and mortality. The current work aimed to evaluate ribosomal RNA gene sequencing for the identification of medically relevant Candida species in comparison with a standard phenotypic method. Eighteen reference strains (RSs), 69 phenotypically identified isolates and 20 inconclusively identified isolates were examined. Internal transcribed spaces (ITSs) and D1/D2 of the 26S ribosomal RNA gene regions were used as targets for sequencing. Additionally, the sequences of the ITS regions were used to establish evolutionary relationships. The sequencing of the ITS regions was successful for 88% (94/107) of the RS and isolates, whereas 100% of the remaining 12% (13/107) of the samples were successfully analysed by sequencing the D1/D2 region. Similarly, genotypic analysis identified all of the RS and isolates, including the 20 isolates that were not phenotypically identified. Phenotypic analysis, however, misidentified 10% (7/69) of the isolates. Phylogenetic analysis allowed the confirmation of the relationships between evolutionarily close species. Currently, the use of genotypic methods is necessary for the correct identification of Candida species.CandidaRibosomal DNADNA sequence analysisCandidaDNA RibossômicoAnálise de Sequência de DNAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11781/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmariana_boité_etal_IOC_2013.pdfapplication/pdf754087https://www.arca.fiocruz.br/bitstream/icict/11781/2/mariana_boit%c3%a9_etal_IOC_2013.pdfd93393de1339222992ebb04d972b71f6MD52TEXTmariana_boité_etal_IOC_2013.pdf.txtmariana_boité_etal_IOC_2013.pdf.txtExtracted texttext/plain43781https://www.arca.fiocruz.br/bitstream/icict/11781/3/mariana_boit%c3%a9_etal_IOC_2013.pdf.txtfc2fc0f72026c803cebe4a57e1d0bfb1MD53icict/117812022-06-24 13:11:04.475oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:11:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
spellingShingle Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
Taverna, Constanza Giselle
Candida
Ribosomal DNA
DNA sequence analysis
Candida
DNA Ribossômico
Análise de Sequência de DNA
title_short Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title_full Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title_fullStr Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title_full_unstemmed Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title_sort Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
author Taverna, Constanza Giselle
author_facet Taverna, Constanza Giselle
Borgeat, María Eugenia Bosco
Murisengo, Omar Alejandro
Davel, Graciela
Boité, Mariana Côrtes
Cupolillo, Elisa
Canteros, Cristina Elena
author_role author
author2 Borgeat, María Eugenia Bosco
Murisengo, Omar Alejandro
Davel, Graciela
Boité, Mariana Côrtes
Cupolillo, Elisa
Canteros, Cristina Elena
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Taverna, Constanza Giselle
Borgeat, María Eugenia Bosco
Murisengo, Omar Alejandro
Davel, Graciela
Boité, Mariana Côrtes
Cupolillo, Elisa
Canteros, Cristina Elena
dc.subject.en.pt_BR.fl_str_mv Candida
Ribosomal DNA
DNA sequence analysis
topic Candida
Ribosomal DNA
DNA sequence analysis
Candida
DNA Ribossômico
Análise de Sequência de DNA
dc.subject.decs.pt_BR.fl_str_mv Candida
DNA Ribossômico
Análise de Sequência de DNA
description Instituto Nacional de Enfermedades Infecciosas Dr Carlos G Malbrán. Departamento Micología. Buenos Aires, Argentina.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-09-21T17:25:46Z
dc.date.available.fl_str_mv 2015-09-21T17:25:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TAVERNA, Constanza Giselle; et al. Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species. Mem Inst Oswaldo Cruz, v.108, n.2, p.178-185, April 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11781
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0074-0276108022013009
identifier_str_mv TAVERNA, Constanza Giselle; et al. Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species. Mem Inst Oswaldo Cruz, v.108, n.2, p.178-185, April 2013.
1678-8060
10.1590/0074-0276108022013009
url https://www.arca.fiocruz.br/handle/icict/11781
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
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instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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