Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species

Detalhes bibliográficos
Autor(a) principal: Taverna,Constanza Giselle
Data de Publicação: 2013
Outros Autores: Bosco-Borgeat,María Eugenia, Murisengo,Omar Alejandro, Davel,Graciela, Boité,Mariana Côrtes, Cupolillo,Elisa, Canteros,Cristina Elena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200178
Resumo: As the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means of accurately and rapidly identifying these species is necessary for the successful early resolution of infection and the subsequent reduction of morbidity and mortality. The current work aimed to evaluate ribosomal RNA gene sequencing for the identification of medically relevant Candida species in comparison with a standard phenotypic method. Eighteen reference strains (RSs), 69 phenotypically identified isolates and 20 inconclusively identified isolates were examined. Internal transcribed spaces (ITSs) and D1/D2 of the 26S ribosomal RNA gene regions were used as targets for sequencing. Additionally, the sequences of the ITS regions were used to establish evolutionary relationships. The sequencing of the ITS regions was successful for 88% (94/107) of the RS and isolates, whereas 100% of the remaining 12% (13/107) of the samples were successfully analysed by sequencing the D1/D2 region. Similarly, genotypic analysis identified all of the RS and isolates, including the 20 isolates that were not phenotypically identified. Phenotypic analysis, however, misidentified 10% (7/69) of the isolates. Phylogenetic analysis allowed the confirmation of the relationships between evolutionarily close species. Currently, the use of genotypic methods is necessary for the correct identification of Candida species.
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spelling Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida speciesCandidaribosomal DNADNA sequence analysisAs the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means of accurately and rapidly identifying these species is necessary for the successful early resolution of infection and the subsequent reduction of morbidity and mortality. The current work aimed to evaluate ribosomal RNA gene sequencing for the identification of medically relevant Candida species in comparison with a standard phenotypic method. Eighteen reference strains (RSs), 69 phenotypically identified isolates and 20 inconclusively identified isolates were examined. Internal transcribed spaces (ITSs) and D1/D2 of the 26S ribosomal RNA gene regions were used as targets for sequencing. Additionally, the sequences of the ITS regions were used to establish evolutionary relationships. The sequencing of the ITS regions was successful for 88% (94/107) of the RS and isolates, whereas 100% of the remaining 12% (13/107) of the samples were successfully analysed by sequencing the D1/D2 region. Similarly, genotypic analysis identified all of the RS and isolates, including the 20 isolates that were not phenotypically identified. Phenotypic analysis, however, misidentified 10% (7/69) of the isolates. Phylogenetic analysis allowed the confirmation of the relationships between evolutionarily close species. Currently, the use of genotypic methods is necessary for the correct identification of Candida species.Instituto Oswaldo Cruz, Ministério da Saúde2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200178Memórias do Instituto Oswaldo Cruz v.108 n.2 2013reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276108022013009info:eu-repo/semantics/openAccessTaverna,Constanza GiselleBosco-Borgeat,María EugeniaMurisengo,Omar AlejandroDavel,GracielaBoité,Mariana CôrtesCupolillo,ElisaCanteros,Cristina Elenaeng2020-04-25T17:51:23Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:52.985Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
spellingShingle Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
Taverna,Constanza Giselle
Candida
ribosomal DNA
DNA sequence analysis
title_short Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title_full Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title_fullStr Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title_full_unstemmed Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
title_sort Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
author Taverna,Constanza Giselle
author_facet Taverna,Constanza Giselle
Bosco-Borgeat,María Eugenia
Murisengo,Omar Alejandro
Davel,Graciela
Boité,Mariana Côrtes
Cupolillo,Elisa
Canteros,Cristina Elena
author_role author
author2 Bosco-Borgeat,María Eugenia
Murisengo,Omar Alejandro
Davel,Graciela
Boité,Mariana Côrtes
Cupolillo,Elisa
Canteros,Cristina Elena
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Taverna,Constanza Giselle
Bosco-Borgeat,María Eugenia
Murisengo,Omar Alejandro
Davel,Graciela
Boité,Mariana Côrtes
Cupolillo,Elisa
Canteros,Cristina Elena
dc.subject.por.fl_str_mv Candida
ribosomal DNA
DNA sequence analysis
topic Candida
ribosomal DNA
DNA sequence analysis
dc.description.none.fl_txt_mv As the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means of accurately and rapidly identifying these species is necessary for the successful early resolution of infection and the subsequent reduction of morbidity and mortality. The current work aimed to evaluate ribosomal RNA gene sequencing for the identification of medically relevant Candida species in comparison with a standard phenotypic method. Eighteen reference strains (RSs), 69 phenotypically identified isolates and 20 inconclusively identified isolates were examined. Internal transcribed spaces (ITSs) and D1/D2 of the 26S ribosomal RNA gene regions were used as targets for sequencing. Additionally, the sequences of the ITS regions were used to establish evolutionary relationships. The sequencing of the ITS regions was successful for 88% (94/107) of the RS and isolates, whereas 100% of the remaining 12% (13/107) of the samples were successfully analysed by sequencing the D1/D2 region. Similarly, genotypic analysis identified all of the RS and isolates, including the 20 isolates that were not phenotypically identified. Phenotypic analysis, however, misidentified 10% (7/69) of the isolates. Phylogenetic analysis allowed the confirmation of the relationships between evolutionarily close species. Currently, the use of genotypic methods is necessary for the correct identification of Candida species.
description As the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means of accurately and rapidly identifying these species is necessary for the successful early resolution of infection and the subsequent reduction of morbidity and mortality. The current work aimed to evaluate ribosomal RNA gene sequencing for the identification of medically relevant Candida species in comparison with a standard phenotypic method. Eighteen reference strains (RSs), 69 phenotypically identified isolates and 20 inconclusively identified isolates were examined. Internal transcribed spaces (ITSs) and D1/D2 of the 26S ribosomal RNA gene regions were used as targets for sequencing. Additionally, the sequences of the ITS regions were used to establish evolutionary relationships. The sequencing of the ITS regions was successful for 88% (94/107) of the RS and isolates, whereas 100% of the remaining 12% (13/107) of the samples were successfully analysed by sequencing the D1/D2 region. Similarly, genotypic analysis identified all of the RS and isolates, including the 20 isolates that were not phenotypically identified. Phenotypic analysis, however, misidentified 10% (7/69) of the isolates. Phylogenetic analysis allowed the confirmation of the relationships between evolutionarily close species. Currently, the use of genotypic methods is necessary for the correct identification of Candida species.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200178
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200178
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-0276108022013009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.108 n.2 2013
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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