Comparative genomics of the anopheline glutathione S-transferase epsilon cluster
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25887 |
Resumo: | No decorrer da pesquisa, a CFJA recebeu o apoio do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) do PDE 201223 / 2007-4. Os financiadores não tiveram nenhum papel no desenho do estudo, coleta e análise de dados, decisão de publicar ou preparação do manuscrito. |
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Ayres, Constância F. J.Müller, PieDyer, NaomiWilding, Craig S.Rigden, Daniel J.Donnelly, Martin J.2018-04-17T13:05:44Z2018-04-17T13:05:44Z2011AYRES, C. et al. Comparative Genomics of the Anopheline Glutathione S-Transferase Epsilon Cluster. PLoS ONE, v. 6, n. 12, p. e29237, 19 dez. 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/2588710.1371/journal.pone.0029237No decorrer da pesquisa, a CFJA recebeu o apoio do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) do PDE 201223 / 2007-4. Os financiadores não tiveram nenhum papel no desenho do estudo, coleta e análise de dados, decisão de publicar ou preparação do manuscrito.Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom / Swiss Tropical & Public Health Institute. Department of Medical Services and Diagnostics. Basel, Switzerland / University of Basel. Basel, Switzerland.Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.University of Liverpool. Institute of Integrative Biology. Liverpool, United Kingdom.Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.Enzymes of the glutathione S-transferase (GST) family play critical roles in detoxification of xenobiotics across many taxa. While GSTs are ubiquitous both in animals and plants, the GST epsilon class (GSTE) is insect-specific and has been associated with resistance to chemical insecticides. While both Aedes aegypti and Anopheles gambiae GSTE clusters consist of eight members, only four putative orthologs are identifiable between the species, suggesting independent expansions of the class in each lineage. We used a primer walking approach, sequencing almost the entire cluster from three Anopheles species (An. stephensi, An. funestus (both Cellia subgenus) and An. plumbeus (Anopheles subgenus)) and compared the sequences to putative orthologs in An. gambiae (Cellia) in an attempt to trace the evolution of the cluster within the subfamily Anophelinae. Furthermore, we measured transcript levels from the identified GSTE loci by real time reverse transcription PCR to determine if all genes were similarly transcribed at different life stages. Among the species investigated, gene order and orientation were similar with three exceptions: (i) GSTE1 was absent in An. plumbeus; (ii) GSTE2 is duplicated in An. plumbeus and (iii) an additional transcriptionally active pseudogene (ψAsGSTE2) was found in An. stephensi. Further statistical analysis and protein modelling gave evidence for positive selection on codons of the catalytic site in GSTE5 albeit its origin seems to predate the introduction of chemical insecticides. Gene expression profiles revealed differences in expression pattern among genes at different life stages. With the exception of GSTE1, ψAsGSTE2 and GSTE2b, all Anopheles species studied share orthologs and hence we assume that GSTE expansion generally predates radiation into subgenera, though the presence of GSTE1 may also suggest a recent duplication event in the Old World Cellia subgenus, instead of a secondary loss. The modifications of the catalytic site within GSTE5 may represent adaptations to new habitats.engEnzimasPCRGenesGenesEnzymesPCR3 'Regiões não traduzidas / genéticaSequência de AminoácidosAnimaisAnopheles / enzimologiaAnopheles / genéticaAnopheles / crescimento e desenvolvimentoEvolução MolecularÉxons / genéticaFêmeaDuplicação GenéticaRegulamento de Expressão Gênica, DesenvolvimentoGenômica / métodosGlutationa transferase / químicaGlutationa transferase / genéticaÍntrons / genéticaModelos, MolecularDados de Sequencia MolecularFamília Multigene / genéticaFilogeniaConformação de ProteínaSeleção GenéticaEspecificidade de EspéciesComparative genomics of the anopheline glutathione S-transferase epsilon clusterinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/25887/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALComparative Genomics of the Anopheline Glutathione.pdfComparative Genomics of the Anopheline Glutathione.pdfapplication/pdf1003367https://www.arca.fiocruz.br/bitstream/icict/25887/2/Comparative%20Genomics%20of%20the%20Anopheline%20Glutathione.pdff83f03fe1a89263f661d3549bf0d5363MD52TEXTComparative Genomics of the Anopheline Glutathione.pdf.txtComparative Genomics of the Anopheline Glutathione.pdf.txtExtracted texttext/plain75748https://www.arca.fiocruz.br/bitstream/icict/25887/3/Comparative%20Genomics%20of%20the%20Anopheline%20Glutathione.pdf.txt511320a732ee6af45591ca1784a0c007MD53icict/258872018-08-15 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| dc.title.pt_BR.fl_str_mv |
Comparative genomics of the anopheline glutathione S-transferase epsilon cluster |
| title |
Comparative genomics of the anopheline glutathione S-transferase epsilon cluster |
| spellingShingle |
Comparative genomics of the anopheline glutathione S-transferase epsilon cluster Ayres, Constância F. J. Enzimas PCR Genes Genes Enzymes PCR 3 'Regiões não traduzidas / genética Sequência de Aminoácidos Animais Anopheles / enzimologia Anopheles / genética Anopheles / crescimento e desenvolvimento Evolução Molecular Éxons / genética Fêmea Duplicação Genética Regulamento de Expressão Gênica, Desenvolvimento Genômica / métodos Glutationa transferase / química Glutationa transferase / genética Íntrons / genética Modelos, Molecular Dados de Sequencia Molecular Família Multigene / genética Filogenia Conformação de Proteína Seleção Genética Especificidade de Espécies |
| title_short |
Comparative genomics of the anopheline glutathione S-transferase epsilon cluster |
| title_full |
Comparative genomics of the anopheline glutathione S-transferase epsilon cluster |
| title_fullStr |
Comparative genomics of the anopheline glutathione S-transferase epsilon cluster |
| title_full_unstemmed |
Comparative genomics of the anopheline glutathione S-transferase epsilon cluster |
| title_sort |
Comparative genomics of the anopheline glutathione S-transferase epsilon cluster |
| author |
Ayres, Constância F. J. |
| author_facet |
Ayres, Constância F. J. Müller, Pie Dyer, Naomi Wilding, Craig S. Rigden, Daniel J. Donnelly, Martin J. |
| author_role |
author |
| author2 |
Müller, Pie Dyer, Naomi Wilding, Craig S. Rigden, Daniel J. Donnelly, Martin J. |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Ayres, Constância F. J. Müller, Pie Dyer, Naomi Wilding, Craig S. Rigden, Daniel J. Donnelly, Martin J. |
| dc.subject.other.pt_BR.fl_str_mv |
Enzimas PCR Genes |
| topic |
Enzimas PCR Genes Genes Enzymes PCR 3 'Regiões não traduzidas / genética Sequência de Aminoácidos Animais Anopheles / enzimologia Anopheles / genética Anopheles / crescimento e desenvolvimento Evolução Molecular Éxons / genética Fêmea Duplicação Genética Regulamento de Expressão Gênica, Desenvolvimento Genômica / métodos Glutationa transferase / química Glutationa transferase / genética Íntrons / genética Modelos, Molecular Dados de Sequencia Molecular Família Multigene / genética Filogenia Conformação de Proteína Seleção Genética Especificidade de Espécies |
| dc.subject.en.pt_BR.fl_str_mv |
Genes Enzymes PCR |
| dc.subject.decs.pt_BR.fl_str_mv |
3 'Regiões não traduzidas / genética Sequência de Aminoácidos Animais Anopheles / enzimologia Anopheles / genética Anopheles / crescimento e desenvolvimento Evolução Molecular Éxons / genética Fêmea Duplicação Genética Regulamento de Expressão Gênica, Desenvolvimento Genômica / métodos Glutationa transferase / química Glutationa transferase / genética Íntrons / genética Modelos, Molecular Dados de Sequencia Molecular Família Multigene / genética Filogenia Conformação de Proteína Seleção Genética Especificidade de Espécies |
| description |
No decorrer da pesquisa, a CFJA recebeu o apoio do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) do PDE 201223 / 2007-4. Os financiadores não tiveram nenhum papel no desenho do estudo, coleta e análise de dados, decisão de publicar ou preparação do manuscrito. |
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2011 |
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2011 |
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2018-04-17T13:05:44Z |
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2018-04-17T13:05:44Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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AYRES, C. et al. Comparative Genomics of the Anopheline Glutathione S-Transferase Epsilon Cluster. PLoS ONE, v. 6, n. 12, p. e29237, 19 dez. 2011. |
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https://www.arca.fiocruz.br/handle/icict/25887 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0029237 |
| identifier_str_mv |
AYRES, C. et al. Comparative Genomics of the Anopheline Glutathione S-Transferase Epsilon Cluster. PLoS ONE, v. 6, n. 12, p. e29237, 19 dez. 2011. 1932-6203 10.1371/journal.pone.0029237 |
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eng |
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eng |
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