Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/56991 |
Resumo: | Oswaldo Cruz Foundation. Instituto Oswaldo Cruz. Department of Immunology. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brasil. |
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Carvalho, Leonardo J. M.Alves, Francisco A.Bianco Jr., CesareOliveira, Salma G.Zanini, Graziela M.Soe, SoeDruilhe, PierreTheisen, MichaelMuniz, José A. P. C.Daniel-Ribeiro, Cláudio T.2023-02-09T19:20:27Z2023-02-09T19:20:27Z2005CARVALHO, Leonardo J. M. et al. Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants. Clinical and Vaccine Immunology, v. 12, n. 2, p. 242-248, 2005.1556-6811https://www.arca.fiocruz.br/handle/icict/5699110.1128/CDLI.12.2.242-248.2005engAmerican Society for MicrobiologyImmunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOswaldo Cruz Foundation. Instituto Oswaldo Cruz. Department of Immunology. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brasil.Oswaldo Cruz Foundation. Instituto Oswaldo Cruz. Department of Immunology. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brasil.Oswaldo Cruz Foundation. Instituto Oswaldo Cruz. Department of Immunology. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brasil.Instituto Evandro Chagas. Belém, PA, Brasil.Oswaldo Cruz Foundation. Evandro Chagas Institute for Clinical Research. Rio de Janeiro, RJ, Brasil.Biomedical Parasitology. Institute Pasteur. Paris, France.Biomedical Parasitology. Institute Pasteur. Paris, France.Clinical Biochemistry. Statens Seruminstitut. Copenhagen, Denmark.Oswaldo Cruz Foundation. Evandro Chagas Institute for Clinical Research. Rio de Janeiro, RJ, Brasil.Oswaldo Cruz Foundation. Instituto Oswaldo Cruz. Department of Immunology. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brasil.The immunogenicity and efficacy of a hybrid recombinant protein derived from the N-terminal end of the glutamate-rich protein (GLURP) and the C-terminal portion of the merozoite surface protein 3 (MSP3) of Plasmodium falciparum was evaluated in Saimiri sciureus monkeys. The GLURP/MSP3 hybrid protein, expressed in Lactococcus lactis, was administered in association with alum, Montanide ISA720, or complete or incomplete Freund adjuvant (CFA/IFA) in groups of five animals each. The three formulations were shown to be immunogenic, but the one with alum was shown to be weak compared to the other two, particularly CFA/IFA, which provided very high antibody titers (enzyme-linked immunosorbent assay titers of >3,000,000 and immunofluorescence antibody test titers of 6,400). After a challenge infection with P. falciparum FUP strain, all five monkeys from the GLURP/MSP3-alum group showed a rapid increase in parasitemia, reaching 10% and were treated early. The two monkeys with the highest antibody titers in group GLURP/MSP3-Montanide ISA720 had a delay in the course of parasitemia and were treated late due to a low hematocrit. In the GLURP/MSP3-CFA/IFA group, parasitemia remained below this threshold in four of the five animals and, after it reached a peak, parasitemia started to decrease and monkeys were treated late. When all animals were grouped according to the outcome, a statistically significant association between high antibody titers and partial protection was observed. The challenge infection boosted the antibody titers, and the importance of this event for vaccine efficacy in areas where this parasite is endemic is discussed. In conclusion, these data suggest that GLURP and MSP3 can induce protection against malaria infection if antibodies are induced at properly high titers.Saimiri sciureus monkeysPlasmodium falciparum antigenProtein 3Immunogenicityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCarvalho_Leonardo_etal_INI_2005.pdfCarvalho_Leonardo_etal_INI_2005.pdfapplication/pdf291685https://www.arca.fiocruz.br/bitstream/icict/56991/2/Carvalho_Leonardo_etal_INI_2005.pdfa8be38eeeb9b5939059b3cfb0e7eee56MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/56991/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/569912023-02-09 16:22:18.454oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-02-09T19:22:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| spellingShingle |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants Carvalho, Leonardo J. M. Saimiri sciureus monkeys Plasmodium falciparum antigen Protein 3 Immunogenicity |
| title_short |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_full |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_fullStr |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_full_unstemmed |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| title_sort |
Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants |
| author |
Carvalho, Leonardo J. M. |
| author_facet |
Carvalho, Leonardo J. M. Alves, Francisco A. Bianco Jr., Cesare Oliveira, Salma G. Zanini, Graziela M. Soe, Soe Druilhe, Pierre Theisen, Michael Muniz, José A. P. C. Daniel-Ribeiro, Cláudio T. |
| author_role |
author |
| author2 |
Alves, Francisco A. Bianco Jr., Cesare Oliveira, Salma G. Zanini, Graziela M. Soe, Soe Druilhe, Pierre Theisen, Michael Muniz, José A. P. C. Daniel-Ribeiro, Cláudio T. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Carvalho, Leonardo J. M. Alves, Francisco A. Bianco Jr., Cesare Oliveira, Salma G. Zanini, Graziela M. Soe, Soe Druilhe, Pierre Theisen, Michael Muniz, José A. P. C. Daniel-Ribeiro, Cláudio T. |
| dc.subject.en.en_US.fl_str_mv |
Saimiri sciureus monkeys Plasmodium falciparum antigen Protein 3 Immunogenicity |
| topic |
Saimiri sciureus monkeys Plasmodium falciparum antigen Protein 3 Immunogenicity |
| description |
Oswaldo Cruz Foundation. Instituto Oswaldo Cruz. Department of Immunology. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2005 |
| dc.date.issued.fl_str_mv |
2005 |
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2023-02-09T19:20:27Z |
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2023-02-09T19:20:27Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
CARVALHO, Leonardo J. M. et al. Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants. Clinical and Vaccine Immunology, v. 12, n. 2, p. 242-248, 2005. |
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https://www.arca.fiocruz.br/handle/icict/56991 |
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1556-6811 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/CDLI.12.2.242-248.2005 |
| identifier_str_mv |
CARVALHO, Leonardo J. M. et al. Immunization of Saimiri sciureus monkeys with a recombinant hybrid protein derived from the Plasmodium falciparum antigen glutamate-rich protein and merozoite surface protein 3 can induce partial protection with Freund and Montanide ISA720 adjuvants. Clinical and Vaccine Immunology, v. 12, n. 2, p. 242-248, 2005. 1556-6811 10.1128/CDLI.12.2.242-248.2005 |
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https://www.arca.fiocruz.br/handle/icict/56991 |
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American Society for Microbiology |
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American Society for Microbiology |
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