Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10704 |
Resumo: | Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil. |
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Bonin, Renata FajardoChapeaurouge, AlexPerales, JonasSilva Jr., José Godinho daNascimento, Hilton Jorge doAssef, Ana Paula D`Alincourt CarvalhoSenna, José Procópio Moreno2015-06-08T14:01:53Z2015-06-08T14:01:53Z2014BONIN, Renata Fajardo et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clin. Appl., v.00, p.1-8, 2014.1862-8354https://www.arca.fiocruz.br/handle/icict/1070410.1002/prca.201300133engWILEY-VCH Verlag GmbH & CoInfecção hospitalarAcinetobacter baumanniiHospital infectionImmunoproteomicsMALDI-TOF/TOFAcinetobacter baumanniiProteômicaIdentification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa de Infecções Hospitalares. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Purpose: Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection. Experimental design: Sera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii,OMPs were separated by 2DE (2Delectrophoresis), and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins. Results: This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth. Conclusions and clinical relevance: Our results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10704/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALanapaula_assefetal_IOC_2014.pdfapplication/pdf1859957https://www.arca.fiocruz.br/bitstream/icict/10704/2/anapaula_assefetal_IOC_2014.pdf09c3b5c760f35acea9aafe7444daa579MD52TEXTanapaula_assefetal_IOC_2014.pdf.txtanapaula_assefetal_IOC_2014.pdf.txtExtracted texttext/plain45683https://www.arca.fiocruz.br/bitstream/icict/10704/3/anapaula_assefetal_IOC_2014.pdf.txt0c940a6591ee92d9787100152381b9faMD53icict/107042022-06-24 12:17:40.186oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach |
title |
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach |
spellingShingle |
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach Bonin, Renata Fajardo Infecção hospitalar Acinetobacter baumannii Hospital infection Immunoproteomics MALDI-TOF/TOF Acinetobacter baumannii Proteômica |
title_short |
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach |
title_full |
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach |
title_fullStr |
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach |
title_full_unstemmed |
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach |
title_sort |
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach |
author |
Bonin, Renata Fajardo |
author_facet |
Bonin, Renata Fajardo Chapeaurouge, Alex Perales, Jonas Silva Jr., José Godinho da Nascimento, Hilton Jorge do Assef, Ana Paula D`Alincourt Carvalho Senna, José Procópio Moreno |
author_role |
author |
author2 |
Chapeaurouge, Alex Perales, Jonas Silva Jr., José Godinho da Nascimento, Hilton Jorge do Assef, Ana Paula D`Alincourt Carvalho Senna, José Procópio Moreno |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Bonin, Renata Fajardo Chapeaurouge, Alex Perales, Jonas Silva Jr., José Godinho da Nascimento, Hilton Jorge do Assef, Ana Paula D`Alincourt Carvalho Senna, José Procópio Moreno |
dc.subject.other.pt_BR.fl_str_mv |
Infecção hospitalar |
topic |
Infecção hospitalar Acinetobacter baumannii Hospital infection Immunoproteomics MALDI-TOF/TOF Acinetobacter baumannii Proteômica |
dc.subject.en.pt_BR.fl_str_mv |
Acinetobacter baumannii Hospital infection Immunoproteomics MALDI-TOF/TOF |
dc.subject.decs.pt_BR.fl_str_mv |
Acinetobacter baumannii Proteômica |
description |
Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2015-06-08T14:01:53Z |
dc.date.available.fl_str_mv |
2015-06-08T14:01:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BONIN, Renata Fajardo et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clin. Appl., v.00, p.1-8, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/10704 |
dc.identifier.issn.pt_BR.fl_str_mv |
1862-8354 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1002/prca.201300133 |
identifier_str_mv |
BONIN, Renata Fajardo et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clin. Appl., v.00, p.1-8, 2014. 1862-8354 10.1002/prca.201300133 |
url |
https://www.arca.fiocruz.br/handle/icict/10704 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
WILEY-VCH Verlag GmbH & Co |
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WILEY-VCH Verlag GmbH & Co |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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