Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach

Detalhes bibliográficos
Autor(a) principal: Bonin, Renata Fajardo
Data de Publicação: 2014
Outros Autores: Chapeaurouge, Alex, Perales, Jonas, Silva Jr., José Godinho da, Nascimento, Hilton Jorge do, Assef, Ana Paula D`Alincourt Carvalho, Senna, José Procópio Moreno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10704
Resumo: Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.
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spelling Bonin, Renata FajardoChapeaurouge, AlexPerales, JonasSilva Jr., José Godinho daNascimento, Hilton Jorge doAssef, Ana Paula D`Alincourt CarvalhoSenna, José Procópio Moreno2015-06-08T14:01:53Z2015-06-08T14:01:53Z2014BONIN, Renata Fajardo et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clin. Appl., v.00, p.1-8, 2014.1862-8354https://www.arca.fiocruz.br/handle/icict/1070410.1002/prca.201300133engWILEY-VCH Verlag GmbH & CoInfecção hospitalarAcinetobacter baumanniiHospital infectionImmunoproteomicsMALDI-TOF/TOFAcinetobacter baumanniiProteômicaIdentification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa de Infecções Hospitalares. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Purpose: Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection. Experimental design: Sera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii,OMPs were separated by 2DE (2Delectrophoresis), and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins. Results: This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth. Conclusions and clinical relevance: Our results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10704/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALanapaula_assefetal_IOC_2014.pdfapplication/pdf1859957https://www.arca.fiocruz.br/bitstream/icict/10704/2/anapaula_assefetal_IOC_2014.pdf09c3b5c760f35acea9aafe7444daa579MD52TEXTanapaula_assefetal_IOC_2014.pdf.txtanapaula_assefetal_IOC_2014.pdf.txtExtracted texttext/plain45683https://www.arca.fiocruz.br/bitstream/icict/10704/3/anapaula_assefetal_IOC_2014.pdf.txt0c940a6591ee92d9787100152381b9faMD53icict/107042022-06-24 12:17:40.186oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
title Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
spellingShingle Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
Bonin, Renata Fajardo
Infecção hospitalar
Acinetobacter baumannii
Hospital infection
Immunoproteomics
MALDI-TOF/TOF
Acinetobacter baumannii
Proteômica
title_short Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
title_full Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
title_fullStr Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
title_full_unstemmed Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
title_sort Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
author Bonin, Renata Fajardo
author_facet Bonin, Renata Fajardo
Chapeaurouge, Alex
Perales, Jonas
Silva Jr., José Godinho da
Nascimento, Hilton Jorge do
Assef, Ana Paula D`Alincourt Carvalho
Senna, José Procópio Moreno
author_role author
author2 Chapeaurouge, Alex
Perales, Jonas
Silva Jr., José Godinho da
Nascimento, Hilton Jorge do
Assef, Ana Paula D`Alincourt Carvalho
Senna, José Procópio Moreno
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bonin, Renata Fajardo
Chapeaurouge, Alex
Perales, Jonas
Silva Jr., José Godinho da
Nascimento, Hilton Jorge do
Assef, Ana Paula D`Alincourt Carvalho
Senna, José Procópio Moreno
dc.subject.other.pt_BR.fl_str_mv Infecção hospitalar
topic Infecção hospitalar
Acinetobacter baumannii
Hospital infection
Immunoproteomics
MALDI-TOF/TOF
Acinetobacter baumannii
Proteômica
dc.subject.en.pt_BR.fl_str_mv Acinetobacter baumannii
Hospital infection
Immunoproteomics
MALDI-TOF/TOF
dc.subject.decs.pt_BR.fl_str_mv Acinetobacter baumannii
Proteômica
description Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:53Z
dc.date.available.fl_str_mv 2015-06-08T14:01:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BONIN, Renata Fajardo et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clin. Appl., v.00, p.1-8, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10704
dc.identifier.issn.pt_BR.fl_str_mv 1862-8354
dc.identifier.doi.pt_BR.fl_str_mv 10.1002/prca.201300133
identifier_str_mv BONIN, Renata Fajardo et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clin. Appl., v.00, p.1-8, 2014.
1862-8354
10.1002/prca.201300133
url https://www.arca.fiocruz.br/handle/icict/10704
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY-VCH Verlag GmbH & Co
publisher.none.fl_str_mv WILEY-VCH Verlag GmbH & Co
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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