Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test

Detalhes bibliográficos
Autor(a) principal: Moraes, Márcia T. B.
Data de Publicação: 1998
Outros Autores: Silva, Messias da, Leite, José Paulo G., Nascimento, Jussara P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43929
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Moraes, Márcia T. B.Silva, Messias daLeite, José Paulo G.Nascimento, Jussara P.2020-10-12T12:42:17Z2020-10-12T12:42:17Z1998MORAES, Márcia T. B. et al. Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n.2, p. 231-235, Mar./Apr. 1998.0074-0276https://www.arca.fiocruz.br/handle/icict/4392910.1590/S0074-027619980002000191678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Análise genética e antigênicaAdenovirus type 3Adenovirus type 3Genetic and antigenic analysisGenetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Testinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.During an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with different enzymes were analyzed and showed a typical Ad3h profile. Using a cross-neutralization test in which both Ad3p and Ad7p antisera were used in different concentration against 100 TCID50 of each adenovirus standard and both isolates, we were able to confirm that the two isolates belong to serotype 3. An hemagglutination inhibition test also corroborated the identification of both strains as adenovirus type 3. Comparing Ad3h and Ad3p genome, we observed 16 different restriction enzyme sites, three of which were located in genomic regions encoding polypeptides involved in neutralization sites.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43929/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarciaTB_Moraes_etal_IOC_1998.pdfMarciaTB_Moraes_etal_IOC_1998.pdfapplication/pdf130878https://www.arca.fiocruz.br/bitstream/icict/43929/2/MarciaTB_Moraes_etal_IOC_1998.pdfc4640671dba85100dee69be84d339b84MD52TEXTMarciaTB_Moraes_etal_IOC_1998.pdf.txtMarciaTB_Moraes_etal_IOC_1998.pdf.txtExtracted texttext/plain19573https://www.arca.fiocruz.br/bitstream/icict/43929/3/MarciaTB_Moraes_etal_IOC_1998.pdf.txt5da2a1eba748e0d796aa8093d7e7fb71MD53icict/439292020-10-13 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dc.title.pt_BR.fl_str_mv Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
title Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
spellingShingle Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
Moraes, Márcia T. B.
Análise genética e antigênica
Adenovirus type 3
Adenovirus type 3
Genetic and antigenic analysis
title_short Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
title_full Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
title_fullStr Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
title_full_unstemmed Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
title_sort Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
author Moraes, Márcia T. B.
author_facet Moraes, Márcia T. B.
Silva, Messias da
Leite, José Paulo G.
Nascimento, Jussara P.
author_role author
author2 Silva, Messias da
Leite, José Paulo G.
Nascimento, Jussara P.
author2_role author
author
author
dc.contributor.author.fl_str_mv Moraes, Márcia T. B.
Silva, Messias da
Leite, José Paulo G.
Nascimento, Jussara P.
dc.subject.other.pt_BR.fl_str_mv Análise genética e antigênica
Adenovirus type 3
topic Análise genética e antigênica
Adenovirus type 3
Adenovirus type 3
Genetic and antigenic analysis
dc.subject.en.pt_BR.fl_str_mv Adenovirus type 3
Genetic and antigenic analysis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 1998
dc.date.issued.fl_str_mv 1998
dc.date.accessioned.fl_str_mv 2020-10-12T12:42:17Z
dc.date.available.fl_str_mv 2020-10-12T12:42:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORAES, Márcia T. B. et al. Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n.2, p. 231-235, Mar./Apr. 1998.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43929
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761998000200019
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MORAES, Márcia T. B. et al. Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n.2, p. 231-235, Mar./Apr. 1998.
0074-0276
10.1590/S0074-02761998000200019
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43929
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/43929/3/MarciaTB_Moraes_etal_IOC_1998.pdf.txt
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