Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells

Detalhes bibliográficos
Autor(a) principal: Peixoto, Mariana Lima Perazzini
Data de Publicação: 2014
Outros Autores: Santos, Dilvani Oliveira, Souza, Ivy de Castro Campos de, Neri, Eloah Christina Lyrio, Sequeira, Danielly Correa Moreira de, De Luca, Paula Mello, Borba, Cintia de Moraes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10707
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
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spelling Peixoto, Mariana Lima PerazziniSantos, Dilvani OliveiraSouza, Ivy de Castro Campos deNeri, Eloah Christina LyrioSequeira, Danielly Correa Moreira deDe Luca, Paula MelloBorba, Cintia de Moraes2015-06-08T14:01:54Z2015-06-08T14:01:54Z2014PEIXOTO, Mariana Lima Perazzini et al. Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells. Revista da Sociedade Brasileira de Medicina Tropical, v.47, n.5, p.613-617, Sep-Oct, 2014.0037-8682https://www.arca.fiocruz.br/handle/icict/1070710.1590/0037-8682-0105-2014engSociedade Brasileira de Medicina TropicalInteraction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Laboratório de Biopatógenos e Ativação Celular. Niterói, RJ, Brasil.Universidade Federal Fluminense. Laboratório de Biopatógenos e Ativação Celular. Niterói, RJ, Brasil.Universidade Federal Fluminense. Laboratório de Biopatógenos e Ativação Celular. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Purpureocillium lilacinum is emerging as a causal agent of hyalohyphomycosis that is refractory to antifungal drugs; however, the pathogenic mechanisms underlying P. lilacinum infection are not understood. In this study, we investigated the interaction of P. lilacinum conidia with human macrophages and dendritic cells in vitro. Methods: Spores of a P. lilacinum clinical isolate were obtained by chill-heat shock. Mononuclear cells were isolated from eight healthy individuals. Monocytes were separated by cold aggregation and differentiated into macrophages by incubation for 7 to 10 days at 37°C or into dendritic cells by the addition of the cytokines human granulocyte-macrophage colony stimulating factor and interleukin-4. Conidial suspension was added to the human cells at 1:1, 2:1, and 5:1 (conidia:cells) ratios for 1h, 6h, and 24h, and the infection was evaluated by Giemsa staining and light microscopy. Results: After 1h interaction, P. lilacinum conidia were internalized by human cells and after 6h contact, some conidia became infl ated. After 24h interaction, the conidia produced germ tubes and hyphae, leading to the disruption of macrophage and dendritic cell membranes. The infection rate analyzed after 6h incubation of P. lilacinum conidia with cells at 2:1 and 1:1 ratios was 76.5% and 25.5%, respectively, for macrophages and 54.3% and 19.5%, respectively, for cultured dendritic cells. Conclusions: P. lilacinum conidia are capable of infecting and destroying both macrophages and dendritic cells, clearly demonstrating the ability of this pathogenic fungus to invade human phagocytic cells.Purpureocillium lilacinumMacrophagesDendritic cellsInteraction in vitroMacrófagosCélulas Dendríticasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10707/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmariana_peixotoetal_IOC_2014.pdfapplication/pdf1206857https://www.arca.fiocruz.br/bitstream/icict/10707/2/mariana_peixotoetal_IOC_2014.pdf60ae36b4af1399c667d4f6006814a29eMD52TEXTmariana_peixotoetal_IOC_2014.pdf.txtmariana_peixotoetal_IOC_2014.pdf.txtExtracted texttext/plain26996https://www.arca.fiocruz.br/bitstream/icict/10707/3/mariana_peixotoetal_IOC_2014.pdf.txta5c19b0566f9aa83b51cb08794b3a321MD53icict/107072022-06-24 13:08:59.083oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
title Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
spellingShingle Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
Peixoto, Mariana Lima Perazzini
Purpureocillium lilacinum
Macrophages
Dendritic cells
Interaction in vitro
Macrófagos
Células Dendríticas
title_short Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
title_full Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
title_fullStr Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
title_full_unstemmed Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
title_sort Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
author Peixoto, Mariana Lima Perazzini
author_facet Peixoto, Mariana Lima Perazzini
Santos, Dilvani Oliveira
Souza, Ivy de Castro Campos de
Neri, Eloah Christina Lyrio
Sequeira, Danielly Correa Moreira de
De Luca, Paula Mello
Borba, Cintia de Moraes
author_role author
author2 Santos, Dilvani Oliveira
Souza, Ivy de Castro Campos de
Neri, Eloah Christina Lyrio
Sequeira, Danielly Correa Moreira de
De Luca, Paula Mello
Borba, Cintia de Moraes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Peixoto, Mariana Lima Perazzini
Santos, Dilvani Oliveira
Souza, Ivy de Castro Campos de
Neri, Eloah Christina Lyrio
Sequeira, Danielly Correa Moreira de
De Luca, Paula Mello
Borba, Cintia de Moraes
dc.subject.en.pt_BR.fl_str_mv Purpureocillium lilacinum
Macrophages
Dendritic cells
Interaction in vitro
topic Purpureocillium lilacinum
Macrophages
Dendritic cells
Interaction in vitro
Macrófagos
Células Dendríticas
dc.subject.decs.pt_BR.fl_str_mv Macrófagos
Células Dendríticas
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:54Z
dc.date.available.fl_str_mv 2015-06-08T14:01:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv PEIXOTO, Mariana Lima Perazzini et al. Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells. Revista da Sociedade Brasileira de Medicina Tropical, v.47, n.5, p.613-617, Sep-Oct, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10707
dc.identifier.issn.pt_BR.fl_str_mv 0037-8682
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0037-8682-0105-2014
identifier_str_mv PEIXOTO, Mariana Lima Perazzini et al. Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells. Revista da Sociedade Brasileira de Medicina Tropical, v.47, n.5, p.613-617, Sep-Oct, 2014.
0037-8682
10.1590/0037-8682-0105-2014
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
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