Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10707 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. |
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Peixoto, Mariana Lima PerazziniSantos, Dilvani OliveiraSouza, Ivy de Castro Campos deNeri, Eloah Christina LyrioSequeira, Danielly Correa Moreira deDe Luca, Paula MelloBorba, Cintia de Moraes2015-06-08T14:01:54Z2015-06-08T14:01:54Z2014PEIXOTO, Mariana Lima Perazzini et al. Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells. Revista da Sociedade Brasileira de Medicina Tropical, v.47, n.5, p.613-617, Sep-Oct, 2014.0037-8682https://www.arca.fiocruz.br/handle/icict/1070710.1590/0037-8682-0105-2014engSociedade Brasileira de Medicina TropicalInteraction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Laboratório de Biopatógenos e Ativação Celular. Niterói, RJ, Brasil.Universidade Federal Fluminense. Laboratório de Biopatógenos e Ativação Celular. Niterói, RJ, Brasil.Universidade Federal Fluminense. Laboratório de Biopatógenos e Ativação Celular. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Purpureocillium lilacinum is emerging as a causal agent of hyalohyphomycosis that is refractory to antifungal drugs; however, the pathogenic mechanisms underlying P. lilacinum infection are not understood. In this study, we investigated the interaction of P. lilacinum conidia with human macrophages and dendritic cells in vitro. Methods: Spores of a P. lilacinum clinical isolate were obtained by chill-heat shock. Mononuclear cells were isolated from eight healthy individuals. Monocytes were separated by cold aggregation and differentiated into macrophages by incubation for 7 to 10 days at 37°C or into dendritic cells by the addition of the cytokines human granulocyte-macrophage colony stimulating factor and interleukin-4. Conidial suspension was added to the human cells at 1:1, 2:1, and 5:1 (conidia:cells) ratios for 1h, 6h, and 24h, and the infection was evaluated by Giemsa staining and light microscopy. Results: After 1h interaction, P. lilacinum conidia were internalized by human cells and after 6h contact, some conidia became infl ated. After 24h interaction, the conidia produced germ tubes and hyphae, leading to the disruption of macrophage and dendritic cell membranes. The infection rate analyzed after 6h incubation of P. lilacinum conidia with cells at 2:1 and 1:1 ratios was 76.5% and 25.5%, respectively, for macrophages and 54.3% and 19.5%, respectively, for cultured dendritic cells. Conclusions: P. lilacinum conidia are capable of infecting and destroying both macrophages and dendritic cells, clearly demonstrating the ability of this pathogenic fungus to invade human phagocytic cells.Purpureocillium lilacinumMacrophagesDendritic cellsInteraction in vitroMacrófagosCélulas Dendríticasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10707/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmariana_peixotoetal_IOC_2014.pdfapplication/pdf1206857https://www.arca.fiocruz.br/bitstream/icict/10707/2/mariana_peixotoetal_IOC_2014.pdf60ae36b4af1399c667d4f6006814a29eMD52TEXTmariana_peixotoetal_IOC_2014.pdf.txtmariana_peixotoetal_IOC_2014.pdf.txtExtracted texttext/plain26996https://www.arca.fiocruz.br/bitstream/icict/10707/3/mariana_peixotoetal_IOC_2014.pdf.txta5c19b0566f9aa83b51cb08794b3a321MD53icict/107072022-06-24 13:08:59.083oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
spellingShingle |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells Peixoto, Mariana Lima Perazzini Purpureocillium lilacinum Macrophages Dendritic cells Interaction in vitro Macrófagos Células Dendríticas |
title_short |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_full |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_fullStr |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_full_unstemmed |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_sort |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
author |
Peixoto, Mariana Lima Perazzini |
author_facet |
Peixoto, Mariana Lima Perazzini Santos, Dilvani Oliveira Souza, Ivy de Castro Campos de Neri, Eloah Christina Lyrio Sequeira, Danielly Correa Moreira de De Luca, Paula Mello Borba, Cintia de Moraes |
author_role |
author |
author2 |
Santos, Dilvani Oliveira Souza, Ivy de Castro Campos de Neri, Eloah Christina Lyrio Sequeira, Danielly Correa Moreira de De Luca, Paula Mello Borba, Cintia de Moraes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Peixoto, Mariana Lima Perazzini Santos, Dilvani Oliveira Souza, Ivy de Castro Campos de Neri, Eloah Christina Lyrio Sequeira, Danielly Correa Moreira de De Luca, Paula Mello Borba, Cintia de Moraes |
dc.subject.en.pt_BR.fl_str_mv |
Purpureocillium lilacinum Macrophages Dendritic cells Interaction in vitro |
topic |
Purpureocillium lilacinum Macrophages Dendritic cells Interaction in vitro Macrófagos Células Dendríticas |
dc.subject.decs.pt_BR.fl_str_mv |
Macrófagos Células Dendríticas |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2015-06-08T14:01:54Z |
dc.date.available.fl_str_mv |
2015-06-08T14:01:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEIXOTO, Mariana Lima Perazzini et al. Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells. Revista da Sociedade Brasileira de Medicina Tropical, v.47, n.5, p.613-617, Sep-Oct, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/10707 |
dc.identifier.issn.pt_BR.fl_str_mv |
0037-8682 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/0037-8682-0105-2014 |
identifier_str_mv |
PEIXOTO, Mariana Lima Perazzini et al. Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells. Revista da Sociedade Brasileira de Medicina Tropical, v.47, n.5, p.613-617, Sep-Oct, 2014. 0037-8682 10.1590/0037-8682-0105-2014 |
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https://www.arca.fiocruz.br/handle/icict/10707 |
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eng |
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eng |
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Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical |
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