Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48251 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.. |
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Moreira, Daniele CorrêaSantos, Arethuza dosMenezes, Rodrigo C.Morgado, Fernanda N.Borba, Cintia M.Ferreira, Joseli Oliveira2021-07-15T14:58:29Z2021-07-15T14:58:29Z2021MOREIRA, Danielly Corrêa et al. Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum. Frontiers in Microbiology. v. 12, Article 615383, p. 1-10, June 2021.1664-302https://www.arca.fiocruz.br/handle/icict/4825110.3389/fmicb.2021.615383engFrontiers MediaPurpureocillium lilacinumResposta imune da peleModelo experimentalImunossupressãoImunohistoquímicaPurpureocillium lilacinumSkin immune responseExperimental modelImmunosuppressionImmunohistochemistrySkin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonose em Animais Domésticos. Rio de Janeiro, RJ, Brasil,Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Hyalohyphomycosis is a fungal infection characterized by the presence of a hyaline mycelium in the host. It is caused by several agents, such as Purpureocillium lilacinum. Our study aimed to evaluate some cell subsets and inflammatory markers involved in the in situ immune response to subcutaneous hyalohyphomycosis by P. lilacinum in C57BL/6 murine models. The fungal isolate was inoculated in mice randomly distributed in immunocompetent/infected (CI) and immunosuppressed/infected (SI) groups. Mice were evaluated on days 1, 3, 5, and 7 after inoculation. Histopathological studies showed several lesions in the site of infection as well as the formation of multifocal and mixed inflammatory infiltrates, which differed between the CI and SI groups. This analysis also revealed conidia and hypha-like structures in subcutaneous tissues of mice of both groups. The immunohistochemical analysis showed lower percentages of macrophages and neutrophils in the SI group compared to those in the CI group. Moreover, the intensity of interleukin (IL)-1β and nitric oxide synthase 2 production by cells of immunosuppressed mice was discreet, compared to immunocompetent mice that ranged from moderate to intense over time. The quantitative interference of dexamethasone in the response to the fungus was also demonstrated. We concluded that our results can be useful not only to broaden the knowledge on P. lilacinum but also, based on this host–parasite relationship, to contribute to the understanding of the mechanisms of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48251/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDaniellyC_Moreira_etal_IOC_2021.pdfDaniellyC_Moreira_etal_IOC_2021.pdfapplication/pdf1244790https://www.arca.fiocruz.br/bitstream/icict/48251/2/DaniellyC_Moreira_etal_IOC_2021.pdf9cf6ec270eaf6d5bf7dcd7c988172479MD52TEXTDaniellyC_Moreira_etal_IOC_2021.pdf.txtDaniellyC_Moreira_etal_IOC_2021.pdf.txtExtracted texttext/plain44410https://www.arca.fiocruz.br/bitstream/icict/48251/3/DaniellyC_Moreira_etal_IOC_2021.pdf.txt8539a562c47b19f3e8c82476f223ff6cMD53icict/482512021-07-16 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| dc.title.pt_BR.fl_str_mv |
Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum |
| title |
Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum |
| spellingShingle |
Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum Moreira, Daniele Corrêa Purpureocillium lilacinum Resposta imune da pele Modelo experimental Imunossupressão Imunohistoquímica Purpureocillium lilacinum Skin immune response Experimental model Immunosuppression Immunohistochemistry |
| title_short |
Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum |
| title_full |
Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum |
| title_fullStr |
Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum |
| title_full_unstemmed |
Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum |
| title_sort |
Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum |
| author |
Moreira, Daniele Corrêa |
| author_facet |
Moreira, Daniele Corrêa Santos, Arethuza dos Menezes, Rodrigo C. Morgado, Fernanda N. Borba, Cintia M. Ferreira, Joseli Oliveira |
| author_role |
author |
| author2 |
Santos, Arethuza dos Menezes, Rodrigo C. Morgado, Fernanda N. Borba, Cintia M. Ferreira, Joseli Oliveira |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Moreira, Daniele Corrêa Santos, Arethuza dos Menezes, Rodrigo C. Morgado, Fernanda N. Borba, Cintia M. Ferreira, Joseli Oliveira |
| dc.subject.other.pt_BR.fl_str_mv |
Purpureocillium lilacinum Resposta imune da pele Modelo experimental Imunossupressão Imunohistoquímica |
| topic |
Purpureocillium lilacinum Resposta imune da pele Modelo experimental Imunossupressão Imunohistoquímica Purpureocillium lilacinum Skin immune response Experimental model Immunosuppression Immunohistochemistry |
| dc.subject.en.pt_BR.fl_str_mv |
Purpureocillium lilacinum Skin immune response Experimental model Immunosuppression Immunohistochemistry |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.. |
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2021 |
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2021-07-15T14:58:29Z |
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2021-07-15T14:58:29Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MOREIRA, Danielly Corrêa et al. Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum. Frontiers in Microbiology. v. 12, Article 615383, p. 1-10, June 2021. |
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https://www.arca.fiocruz.br/handle/icict/48251 |
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1664-302 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2021.615383 |
| identifier_str_mv |
MOREIRA, Danielly Corrêa et al. Skin Immune Response of Immunocompetent and Immunosuppressed C57BL/6 Mice After Experimental Subcutaneous Infection Caused by Purpureocillium lilacinum. Frontiers in Microbiology. v. 12, Article 615383, p. 1-10, June 2021. 1664-302 10.3389/fmicb.2021.615383 |
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Frontiers Media |
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Frontiers Media |
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