PCR-RFLP assay as an option for primary HPV test
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28878 |
Resumo: | Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Microbiologia e Sorologia. Florianópolis, SC, Brasil |
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Golfetto, LisléiaAlves, Eduardo VenâncioMartins, Toni RicardoSincero, Thais Cristine MarquesCastro, J.B.S.Dannebrock, ChristianOliveira, Jaquelline Germano deLevi, Jose EduardoOnofre, Alexandre Sherlley CasimiroBazzo, Maria Luiza2018-09-19T11:56:47Z2018-09-19T11:56:47Z2018GOLFETTO, Lisléia et al. PCR-RFLP assay as an option for primary HPV test. Braz J Med Biol Res, v. 51, n. 5, e7098, 2018.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/2887810.1590/1414-431x20177098engAssociação Brasileira de Divulgação Científicacancer cervicalHPVTriagemPCR-RFLPMicroarranjo PapilloCheck®Cervical cancerHPVScreeningPCR-RFLPPapilloCheck® microarrayPCR-RFLP assay as an option for primary HPV testinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Microbiologia e Sorologia. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Microbiologia e Sorologia. Florianópolis, SC, BrasilUniversidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Virologia. São Paulo, SP, BrasilUniversidade Federal de Santa Catarina. Departamento de Análises Clínicas. Florianópolis, SC, BrasilPrefeitura de São Miguel do Oeste. Secretaria de Saúde. Posto Central. São Miguel do Oeste, SC, BrasilLaboratório Prevent Citopatologia. São Miguel do Oeste, SC, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Imunologia Celular e Molecular. Belo Horizonte, MG, BrasilUniversidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Virologia. São Paulo, SP, BrasilUniversidade Federal de Santa Catarina. Departamento de Análises Clínicas. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Microbiologia e Sorologia. Florianópolis, SC, Brasil/Universidade Federal de Santa Catarina. Departamento de Análises Clínicas. Florianópolis, SC, BrasilPersistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24.6%) were positive for HPV gene by PCR-Multiplex and were then submitted to PCR-RFLP and PapilloCheck® microarray. There was a genotyping agreement in 71.25% (57/80) on at least one HPV type between PCR-RFLP and PapilloCheck® microarray. In 22 samples (27.5%), the results were discordant and those samples were additionally analyzed by DNA sequencing. HPV 16 was the most prevalent HPV type found in both methods, followed by HPVs 53, 68, 18, 39, and 66 using PCR-RFLP analysis, and HPVs 39, 53, 68, 56, 31, and 66 using PapilloCheck® microarray. In the present study, a perfect agreement using Cohen's kappa (κ) was found in HPV 33 and 58 (κ=1), very good for HPV 51, and good for types 16, 18, 53, 59, 66, 68, 70, and 73. PCR-RFLP analysis identified only 25% (20/80) HPV coinfection, and PapilloCheck® microarray found 62.5% (50/80). Our Cohen's kappa results indicate that our in-house HPV genotyping testing (PCR-RFLP analysis) could be applied as a primary HPV test screening, especially in low income countries. If multiple HPV types are found in this primary test, a more descriptive test, such as PapilloCheck® microarray, could be performed.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/28878/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALPCR-RFLP assay.pdfPCR-RFLP assay.pdfapplication/pdf192291https://www.arca.fiocruz.br/bitstream/icict/28878/2/PCR-RFLP%20assay.pdfb74efe965a98dad99302b9fbcd713ecbMD52TEXTPCR-RFLP assay.pdf.txtPCR-RFLP assay.pdf.txtExtracted texttext/plain31635https://www.arca.fiocruz.br/bitstream/icict/28878/3/PCR-RFLP%20assay.pdf.txt4f27bdc39ee417aafa4d4200e0e82efdMD53icict/288782019-06-19 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| dc.title.pt_BR.fl_str_mv |
PCR-RFLP assay as an option for primary HPV test |
| title |
PCR-RFLP assay as an option for primary HPV test |
| spellingShingle |
PCR-RFLP assay as an option for primary HPV test Golfetto, Lisléia cancer cervical HPV Triagem PCR-RFLP Microarranjo PapilloCheck® Cervical cancer HPV Screening PCR-RFLP PapilloCheck® microarray |
| title_short |
PCR-RFLP assay as an option for primary HPV test |
| title_full |
PCR-RFLP assay as an option for primary HPV test |
| title_fullStr |
PCR-RFLP assay as an option for primary HPV test |
| title_full_unstemmed |
PCR-RFLP assay as an option for primary HPV test |
| title_sort |
PCR-RFLP assay as an option for primary HPV test |
| author |
Golfetto, Lisléia |
| author_facet |
Golfetto, Lisléia Alves, Eduardo Venâncio Martins, Toni Ricardo Sincero, Thais Cristine Marques Castro, J.B.S. Dannebrock, Christian Oliveira, Jaquelline Germano de Levi, Jose Eduardo Onofre, Alexandre Sherlley Casimiro Bazzo, Maria Luiza |
| author_role |
author |
| author2 |
Alves, Eduardo Venâncio Martins, Toni Ricardo Sincero, Thais Cristine Marques Castro, J.B.S. Dannebrock, Christian Oliveira, Jaquelline Germano de Levi, Jose Eduardo Onofre, Alexandre Sherlley Casimiro Bazzo, Maria Luiza |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Golfetto, Lisléia Alves, Eduardo Venâncio Martins, Toni Ricardo Sincero, Thais Cristine Marques Castro, J.B.S. Dannebrock, Christian Oliveira, Jaquelline Germano de Levi, Jose Eduardo Onofre, Alexandre Sherlley Casimiro Bazzo, Maria Luiza |
| dc.subject.other.pt_BR.fl_str_mv |
cancer cervical HPV Triagem PCR-RFLP Microarranjo PapilloCheck® |
| topic |
cancer cervical HPV Triagem PCR-RFLP Microarranjo PapilloCheck® Cervical cancer HPV Screening PCR-RFLP PapilloCheck® microarray |
| dc.subject.en.pt_BR.fl_str_mv |
Cervical cancer HPV Screening PCR-RFLP PapilloCheck® microarray |
| description |
Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Microbiologia e Sorologia. Florianópolis, SC, Brasil |
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2018 |
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2018-09-19T11:56:47Z |
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2018-09-19T11:56:47Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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GOLFETTO, Lisléia et al. PCR-RFLP assay as an option for primary HPV test. Braz J Med Biol Res, v. 51, n. 5, e7098, 2018. |
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https://www.arca.fiocruz.br/handle/icict/28878 |
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0100-879X |
| dc.identifier.doi.none.fl_str_mv |
10.1590/1414-431x20177098 |
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GOLFETTO, Lisléia et al. PCR-RFLP assay as an option for primary HPV test. Braz J Med Biol Res, v. 51, n. 5, e7098, 2018. 0100-879X 10.1590/1414-431x20177098 |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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