Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain

Detalhes bibliográficos
Autor(a) principal: Decco, Danielle Angst
Data de Publicação: 2014
Outros Autores: Balassiano, Ilana Teruskin, Boente, Renata Ferreira, Miranda, Karla Rodrigues, Brazier, Jon, Hall, Val, Santos Filho, Joaquim dos, Lobo, Leandro Araújo, Nouér, Simone Aranha, Domingues, Regina Maria Cavalcanti Pilotto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10957
Resumo: Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes . Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.
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spelling Decco, Danielle AngstBalassiano, Ilana TeruskinBoente, Renata FerreiraMiranda, Karla RodriguesBrazier, JonHall, ValSantos Filho, Joaquim dosLobo, Leandro AraújoNouér, Simone AranhaDomingues, Regina Maria Cavalcanti Pilotto2015-06-24T12:26:36Z2015-06-24T12:26:36Z2014SECCO, Danielle Angst et al. Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain. Anaerobe, v.28, p.85-89, 2014.1075-9964https://www.arca.fiocruz.br/handle/icict/109570.1016/j.anaerobe.2014.05.013engElsevierBactériaResistência a medicamentosClostridium difficileCDINosocomial infectionMoxifloxacin resistanceClostridium difficileClostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 straininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes . Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes . Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes . Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.University Hospital of Wales. Public Health Wales Microbiology. Anaerobe Reference Unit. Cardiff, Wales, UK.University Hospital of Wales. Public Health Wales Microbiology. Anaerobe Reference Unit. Cardiff, Wales, UK.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes . Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes . Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro.Hospital Universitário Clementino Fraga Filho. Coordenação de Controle de Infecções Hospitalares. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes . Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Clostridium difficile is a Gram-positive spore forming anaerobic bacterium, often associated with nosocomial diarrhea and pseudomembranous colitis. The acquisition of this organism occurs primarily in hospitals through accidental ingestion of spores, and its establishment and proliferation in the colon results from the removal of members of the normal intestinal flora during or after antibiotic therapy. In this study, stool samples from patients admitted to the University Hospital Clementino Fraga Filho (HUCCF/UFRJ) were screened for C. difficile toxins with an ELISA test and cultured with standard techniques for C. difficile isolation. A total of 74 stool samples were collected from patients undergoing antibiotic therapy between August 2009 and November 2010, only two (2.7%) were positive in the ELISA test and culture. A third isolate was obtained from a negative ELISA test sample. All cases of CDI were identified in patients with acute lymphoid or myeloid leukemia. Genotypic and phenotypic characterization showed that all strains carried toxins A and B genes, and belonged to PCR-ribotypes 014, 043 and 046. The isolated strains were sensitive to metronidazole and vancomycin, and resistant to ciprofloxacin and levofloxacin. Resistance to moxifloxacin, was present in the strain from PCR-ribotype 014, that showed an amino acid substitution in gyrB gene (Asp 426 → Asn). This is the first time that this mutation in a PCR-ribotype 014 strain has been described in Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10957/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALdanielle_seccoetal_IOC_2014.pdfapplication/pdf300153https://www.arca.fiocruz.br/bitstream/icict/10957/2/danielle_seccoetal_IOC_2014.pdfe999ac0f5d596e556da1e8a9d76798b4MD52TEXTdanielle_seccoetal_IOC_2014.pdf.txtdanielle_seccoetal_IOC_2014.pdf.txtExtracted texttext/plain31959https://www.arca.fiocruz.br/bitstream/icict/10957/3/danielle_seccoetal_IOC_2014.pdf.txt27f55b363657b8452632850f585fccabMD53icict/109572022-06-24 13:02:55.902oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:02:55Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain
title Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain
spellingShingle Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain
Decco, Danielle Angst
Bactéria
Resistência a medicamentos
Clostridium difficile
CDI
Nosocomial infection
Moxifloxacin resistance
Clostridium difficile
title_short Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain
title_full Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain
title_fullStr Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain
title_full_unstemmed Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain
title_sort Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain
author Decco, Danielle Angst
author_facet Decco, Danielle Angst
Balassiano, Ilana Teruskin
Boente, Renata Ferreira
Miranda, Karla Rodrigues
Brazier, Jon
Hall, Val
Santos Filho, Joaquim dos
Lobo, Leandro Araújo
Nouér, Simone Aranha
Domingues, Regina Maria Cavalcanti Pilotto
author_role author
author2 Balassiano, Ilana Teruskin
Boente, Renata Ferreira
Miranda, Karla Rodrigues
Brazier, Jon
Hall, Val
Santos Filho, Joaquim dos
Lobo, Leandro Araújo
Nouér, Simone Aranha
Domingues, Regina Maria Cavalcanti Pilotto
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Decco, Danielle Angst
Balassiano, Ilana Teruskin
Boente, Renata Ferreira
Miranda, Karla Rodrigues
Brazier, Jon
Hall, Val
Santos Filho, Joaquim dos
Lobo, Leandro Araújo
Nouér, Simone Aranha
Domingues, Regina Maria Cavalcanti Pilotto
dc.subject.other.pt_BR.fl_str_mv Bactéria
Resistência a medicamentos
topic Bactéria
Resistência a medicamentos
Clostridium difficile
CDI
Nosocomial infection
Moxifloxacin resistance
Clostridium difficile
dc.subject.en.pt_BR.fl_str_mv Clostridium difficile
CDI
Nosocomial infection
Moxifloxacin resistance
dc.subject.decs.pt_BR.fl_str_mv Clostridium difficile
description Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes . Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-24T12:26:36Z
dc.date.available.fl_str_mv 2015-06-24T12:26:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SECCO, Danielle Angst et al. Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain. Anaerobe, v.28, p.85-89, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10957
dc.identifier.issn.pt_BR.fl_str_mv 1075-9964
dc.identifier.doi.pt_BR.fl_str_mv 0.1016/j.anaerobe.2014.05.013
identifier_str_mv SECCO, Danielle Angst et al. Clostridium difficile infection among immunocompromised patients in Rio de Janeiro, Brazil and detection of moxifloxacin resistance in a ribotype 014 strain. Anaerobe, v.28, p.85-89, 2014.
1075-9964
0.1016/j.anaerobe.2014.05.013
url https://www.arca.fiocruz.br/handle/icict/10957
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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