The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18220 |
Resumo: | Universidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil. |
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Santos, Dilvani OliveiraCoelho, Janaína GuimarãesNeri, Eloah Christina LyrioSouza, Ivy de Castro CamposPonte, Carlos Germano GarridoAntas, Paulo Renato Zuquim2017-03-30T16:25:46Z2017-03-30T16:25:46Z2016SANTOS, Dilvani Oliveira; et al. The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion. Journal of Clinical & Cellular Immunology, v.7:4, 8p, Aug. 20162155-9899https://www.arca.fiocruz.br/handle/icict/18220engOMICS InternationalHanseníaseBCGMonócitosApresentação do antígenoCitocinasAdesão celularLeprosyBCGAntigen presentationMonocyteCytokineCell adhesionThe Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil.Universidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil.Universidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil.Universidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil.Clinical manifestations in leprosy, a chronic infectious disease caused by Mycobacterium leprae, depend on the immunologic reactions of the patient to the bacilli. Previously, we have shown a reversion of the inefficiency of the cellular-mediated immune response against M. leprae in lepromatous leprosy patients when dendritic cells (DCs) were used in vitro as APC. The aim of the current study is to investigate the cell-to-cell interaction when purified human monocytes-derived DCs and macrophages from healthy adult donors were co-cultured with autologous lymphocytes in the presence of either M. leprae or M. bovis BCG by using APCs which were either attached or in suspension. Subsequently, APCs were analyzed by optical microscopy, phenotypically monitored by flow cytometry, and cytokines secretion evaluated by ELISA and Luminex. Overall, our results suggest that: (1) the process of adhesion of the APC may not be absolutely essential for antigen presentation activity; (2) IL-12 production appears to be in direct relation to the susceptibility of the host cell to infection with M. leprae; (3) in the presence of autologous lymphocytes, there is a down-modulation of surface markers studied, except for CD209 in M. leprae-infected CD11c+ DCs, although a similar decreased expression of these markers on macrophages was also observed, and this step occurs in an antigen-dependent manner; and (4) the cytokines secretion is also dependent on the APCs’ adhesion process. In parallel, the human THP-1 cell line was assessed showing that no monokine was differentially regulated when cells remained either attached or in suspension, regardless of the stimulus employed. These results may further guide future pivotal experimental procedures when studying in vitro cellular immune responses against mycobacteria.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18220/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALpaulo2_antas_etal_IOC-2016.pdfpaulo2_antas_etal_IOC-2016.pdfapplication/pdf1463259https://www.arca.fiocruz.br/bitstream/icict/18220/2/paulo2_antas_etal_IOC-2016.pdfb8f0c270ba38bd89e2e76d6c8f419763MD52TEXTpaulo2_antas_etal_IOC-2016.pdf.txtpaulo2_antas_etal_IOC-2016.pdf.txtExtracted texttext/plain54538https://www.arca.fiocruz.br/bitstream/icict/18220/3/paulo2_antas_etal_IOC-2016.pdf.txtdb978e483e14ce422fbc28ec81ec1f45MD53icict/182202018-08-15 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| dc.title.pt_BR.fl_str_mv |
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion |
| title |
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion |
| spellingShingle |
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion Santos, Dilvani Oliveira Hanseníase BCG Monócitos Apresentação do antígeno Citocinas Adesão celular Leprosy BCG Antigen presentation Monocyte Cytokine Cell adhesion |
| title_short |
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion |
| title_full |
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion |
| title_fullStr |
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion |
| title_full_unstemmed |
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion |
| title_sort |
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion |
| author |
Santos, Dilvani Oliveira |
| author_facet |
Santos, Dilvani Oliveira Coelho, Janaína Guimarães Neri, Eloah Christina Lyrio Souza, Ivy de Castro Campos Ponte, Carlos Germano Garrido Antas, Paulo Renato Zuquim |
| author_role |
author |
| author2 |
Coelho, Janaína Guimarães Neri, Eloah Christina Lyrio Souza, Ivy de Castro Campos Ponte, Carlos Germano Garrido Antas, Paulo Renato Zuquim |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Dilvani Oliveira Coelho, Janaína Guimarães Neri, Eloah Christina Lyrio Souza, Ivy de Castro Campos Ponte, Carlos Germano Garrido Antas, Paulo Renato Zuquim |
| dc.subject.other.pt_BR.fl_str_mv |
Hanseníase BCG Monócitos Apresentação do antígeno Citocinas Adesão celular |
| topic |
Hanseníase BCG Monócitos Apresentação do antígeno Citocinas Adesão celular Leprosy BCG Antigen presentation Monocyte Cytokine Cell adhesion |
| dc.subject.en.pt_BR.fl_str_mv |
Leprosy BCG Antigen presentation Monocyte Cytokine Cell adhesion |
| description |
Universidade Federal Fluminense. Depto. de Biologia Celular e Molecular. Laboratório de Biopatógenos e Ativação Celular e Processos Educativos (LaBiopAC&ProEduc). Niterói, RJ, Brasil. |
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2016 |
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2016 |
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2017-03-30T16:25:46Z |
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2017-03-30T16:25:46Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SANTOS, Dilvani Oliveira; et al. The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion. Journal of Clinical & Cellular Immunology, v.7:4, 8p, Aug. 2016 |
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https://www.arca.fiocruz.br/handle/icict/18220 |
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2155-9899 |
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SANTOS, Dilvani Oliveira; et al. The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion. Journal of Clinical & Cellular Immunology, v.7:4, 8p, Aug. 2016 2155-9899 |
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