Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure

Detalhes bibliográficos
Autor(a) principal: Lachtermacher, S.
Data de Publicação: 2010
Outros Autores: Esporcatte, B. L. B., Montalvão, F., Costa, P. C., Rodrigues, D. C., Belem, L., Rabischoffisky, A., Faria Neto, Hugo C. C., Vasconcellos, R., Iacobas, S., Dohmann, H. F. R., Spray, D. C., Goldenberg, R. C. S., Carvalho, A. C. Campos de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30450
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
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spelling Lachtermacher, S.Esporcatte, B. L. B.Montalvão, F.Costa, P. C.Rodrigues, D. C.Belem, L.Rabischoffisky, A.Faria Neto, Hugo C. C.Vasconcellos, R.Iacobas, S.Dohmann, H. F. R.Spray, D. C.Goldenberg, R. C. S.Carvalho, A. C. Campos de2018-12-09T18:10:01Z2018-12-09T18:10:01Z2010LACHTERMACHER, S. et al. Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure. Brazilian Journal of Medical and Biological Research, v.43, p.377-389, 2010.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/30450engAssociação Brasileira de Divulgação CientíficaInsuficiência cardíaca pós-isquêmica experimentalAnticorpos anti-coraçãoCitocinasExperimental post-ischemic heart failureAnti-heart antibodiesCytokinesImmunoarrayRNAm - MicroarrayRNA MensageiroCardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Centro de Ensino e Pesquisa, Hospital Pró-Cardíaco, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto de Imunologia. Departamento de Imunobiologia. Niterói, RJ. Brasil.Albert Einstein College of Medicine. Bronx, NY, USA.Albert Einstein College of Medicine. Bronx, NY, USA.Centro de Ensino e Pesquisa, Hospital Pró-Cardíaco, Rio de Janeiro, RJ, Brasil.Albert Einstein College of Medicine. Bronx, NY, USA.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Albert Einstein College of Medicine. Bronx, NY, USA.After myocardial infarction (MI), activation of the immune system and inflammatory mechanisms, among others, can lead to ventricular remodeling and heart failure (HF). The interaction between these systemic alterations and corresponding changes in the heart has not been extensively examined in the setting of chronic ischemia. The main purpose of this study was to investigate alterations in cardiac gene and systemic cytokine profile in mice with post-ischemic HF. Plasma was tested for IgM and IgG anti-heart reactive repertoire and inflammatory cytokines. Heart samples were assayed for gene expression by analyzing hybridization to AECOM 32k mouse microarrays. Ischemic HF significantly increased the levels of total serum IgM (by 5.2-fold) and total IgG (by 3.6-fold) associated with a relatively high content of anti-heart specificity. A comparable increase was observed in the levels of circulating pro-inflammatory cytokines such as IL-1β (3.8X) and TNF-α (6.0X). IFN-γ was also increased by 3.1-fold in the MI group. However, IL-4 and IL-10 were not significantly different between the MI and sham-operated groups. Chemokines such as MCP-1 and IL-8 were 1.4- and 13-fold increased, respectively, in the plasma of infarcted mice. We identified 2079 well annotated unigenes that were significantly regulated by post-ischemic HF. Complement activation and immune response were among the most up-regulated processes. Interestingly, 21 of the 101 quantified unigenes involved in the inflammatory response were significantly up-regulated and none were down-regulated. These data indicate that post-ischemic heart remodeling is accompanied by immune-mediated mechanisms that act both systemically and locally.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30450/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALhugocc2_farianeto_etal_IOC_2010.pdfhugocc2_farianeto_etal_IOC_2010.pdfapplication/pdf1188349https://www.arca.fiocruz.br/bitstream/icict/30450/2/hugocc2_farianeto_etal_IOC_2010.pdf8fd97f76f99ffad2ede3b0be771b7d34MD52TEXThugocc2_farianeto_etal_IOC_2010.pdf.txthugocc2_farianeto_etal_IOC_2010.pdf.txtExtracted texttext/plain60228https://www.arca.fiocruz.br/bitstream/icict/30450/3/hugocc2_farianeto_etal_IOC_2010.pdf.txt719c41cf9f85454d2acf573180e1ec4eMD53icict/304502018-12-10 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dc.title.pt_BR.fl_str_mv Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
title Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
spellingShingle Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
Lachtermacher, S.
Insuficiência cardíaca pós-isquêmica experimental
Anticorpos anti-coração
Citocinas
Experimental post-ischemic heart failure
Anti-heart antibodies
Cytokines
Immunoarray
RNAm - Microarray
RNA Mensageiro
title_short Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
title_full Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
title_fullStr Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
title_full_unstemmed Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
title_sort Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
author Lachtermacher, S.
author_facet Lachtermacher, S.
Esporcatte, B. L. B.
Montalvão, F.
Costa, P. C.
Rodrigues, D. C.
Belem, L.
Rabischoffisky, A.
Faria Neto, Hugo C. C.
Vasconcellos, R.
Iacobas, S.
Dohmann, H. F. R.
Spray, D. C.
Goldenberg, R. C. S.
Carvalho, A. C. Campos de
author_role author
author2 Esporcatte, B. L. B.
Montalvão, F.
Costa, P. C.
Rodrigues, D. C.
Belem, L.
Rabischoffisky, A.
Faria Neto, Hugo C. C.
Vasconcellos, R.
Iacobas, S.
Dohmann, H. F. R.
Spray, D. C.
Goldenberg, R. C. S.
Carvalho, A. C. Campos de
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lachtermacher, S.
Esporcatte, B. L. B.
Montalvão, F.
Costa, P. C.
Rodrigues, D. C.
Belem, L.
Rabischoffisky, A.
Faria Neto, Hugo C. C.
Vasconcellos, R.
Iacobas, S.
Dohmann, H. F. R.
Spray, D. C.
Goldenberg, R. C. S.
Carvalho, A. C. Campos de
dc.subject.other.pt_BR.fl_str_mv Insuficiência cardíaca pós-isquêmica experimental
Anticorpos anti-coração
Citocinas
topic Insuficiência cardíaca pós-isquêmica experimental
Anticorpos anti-coração
Citocinas
Experimental post-ischemic heart failure
Anti-heart antibodies
Cytokines
Immunoarray
RNAm - Microarray
RNA Mensageiro
dc.subject.en.pt_BR.fl_str_mv Experimental post-ischemic heart failure
Anti-heart antibodies
Cytokines
Immunoarray
RNAm - Microarray
dc.subject.decs.pt_BR.fl_str_mv RNA Mensageiro
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-12-09T18:10:01Z
dc.date.available.fl_str_mv 2018-12-09T18:10:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LACHTERMACHER, S. et al. Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure. Brazilian Journal of Medical and Biological Research, v.43, p.377-389, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30450
dc.identifier.issn.pt_BR.fl_str_mv 0100-879X
identifier_str_mv LACHTERMACHER, S. et al. Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure. Brazilian Journal of Medical and Biological Research, v.43, p.377-389, 2010.
0100-879X
url https://www.arca.fiocruz.br/handle/icict/30450
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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