Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/19665 |
Resumo: | Objective - To assess the transfection of the gene that encodes green fluorescent protein (GFP) through direct intramyocardial injection. Methods - The pREGFP plasmid vector was used. The EGFP gene was inserted downstream from the constitutive promoter of the Rous sarcoma virus. Five male dogs were used (mean weight 13.5 kg), in which 0.5 mL of saline solution (n=1) or 0.5 mL of plasmid solution containing 0.5 μg of pREGFP/dog (n=4) were injected into the myocardium of the left ventricular lateral wall. The dogs were euthanized 1 week later, and cardiac biopsies were obtained. Results - Fluorescence microscopy showed differences between the cells transfected and not transfected with pREGFP plasmid. Mild fluorescence was observed in the cardiac fibers that received saline solution; however, the myocardial cells transfected with pREGFP had overt EGFP expression. Conclusion - Transfection with the EGFP gene in healthy canine myocardium was effective. The reproduction of this efficacy using vascular endothelial growth factor (VEGF) instead of EGFP aims at developing gene therapy for ischemic heart disease. |
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Kalil, Renato Abdala KaramTeixeira, Leonardo Augusto KaramMastalir, Eduardo T.Moreno, Paulo Lavaniere de AzevedoFricke, Cecilia HelenaNardi, Nance Beyer2010-04-16T09:11:07Z20020066-782Xhttp://hdl.handle.net/10183/19665000372497Objective - To assess the transfection of the gene that encodes green fluorescent protein (GFP) through direct intramyocardial injection. Methods - The pREGFP plasmid vector was used. The EGFP gene was inserted downstream from the constitutive promoter of the Rous sarcoma virus. Five male dogs were used (mean weight 13.5 kg), in which 0.5 mL of saline solution (n=1) or 0.5 mL of plasmid solution containing 0.5 μg of pREGFP/dog (n=4) were injected into the myocardium of the left ventricular lateral wall. The dogs were euthanized 1 week later, and cardiac biopsies were obtained. Results - Fluorescence microscopy showed differences between the cells transfected and not transfected with pREGFP plasmid. Mild fluorescence was observed in the cardiac fibers that received saline solution; however, the myocardial cells transfected with pREGFP had overt EGFP expression. Conclusion - Transfection with the EGFP gene in healthy canine myocardium was effective. The reproduction of this efficacy using vascular endothelial growth factor (VEGF) instead of EGFP aims at developing gene therapy for ischemic heart disease.application/pdfengArquivos brasileiros de cardiologia. São Paulo. Vol. 79, n. 3 (2002), p. 228-232Terapia gênica : Cura : DoençasIsquemiaCoraçãoTransferência genéticaGene therapyGene transfectionIschemic heart diseaseExperimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart diseaseinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000372497.pdf000372497.pdfTexto completo (inglês)application/pdf210209http://www.lume.ufrgs.br/bitstream/10183/19665/1/000372497.pdfbf4cdbe838cd8f19ac90005c45c48342MD51TEXT000372497.pdf.txt000372497.pdf.txtExtracted Texttext/plain21311http://www.lume.ufrgs.br/bitstream/10183/19665/2/000372497.pdf.txt4047df81a80a6c6cd9a47eedb0c9dee0MD52THUMBNAIL000372497.pdf.jpg000372497.pdf.jpgGenerated Thumbnailimage/jpeg2079http://www.lume.ufrgs.br/bitstream/10183/19665/3/000372497.pdf.jpgfe0fa2ca0a4fde52102b3836a38d582bMD5310183/196652021-06-26 04:48:09.574247oai:www.lume.ufrgs.br:10183/19665Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-06-26T07:48:09Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease |
title |
Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease |
spellingShingle |
Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease Kalil, Renato Abdala Karam Terapia gênica : Cura : Doenças Isquemia Coração Transferência genética Gene therapy Gene transfection Ischemic heart disease |
title_short |
Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease |
title_full |
Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease |
title_fullStr |
Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease |
title_full_unstemmed |
Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease |
title_sort |
Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease |
author |
Kalil, Renato Abdala Karam |
author_facet |
Kalil, Renato Abdala Karam Teixeira, Leonardo Augusto Karam Mastalir, Eduardo T. Moreno, Paulo Lavaniere de Azevedo Fricke, Cecilia Helena Nardi, Nance Beyer |
author_role |
author |
author2 |
Teixeira, Leonardo Augusto Karam Mastalir, Eduardo T. Moreno, Paulo Lavaniere de Azevedo Fricke, Cecilia Helena Nardi, Nance Beyer |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Kalil, Renato Abdala Karam Teixeira, Leonardo Augusto Karam Mastalir, Eduardo T. Moreno, Paulo Lavaniere de Azevedo Fricke, Cecilia Helena Nardi, Nance Beyer |
dc.subject.por.fl_str_mv |
Terapia gênica : Cura : Doenças Isquemia Coração Transferência genética |
topic |
Terapia gênica : Cura : Doenças Isquemia Coração Transferência genética Gene therapy Gene transfection Ischemic heart disease |
dc.subject.eng.fl_str_mv |
Gene therapy Gene transfection Ischemic heart disease |
description |
Objective - To assess the transfection of the gene that encodes green fluorescent protein (GFP) through direct intramyocardial injection. Methods - The pREGFP plasmid vector was used. The EGFP gene was inserted downstream from the constitutive promoter of the Rous sarcoma virus. Five male dogs were used (mean weight 13.5 kg), in which 0.5 mL of saline solution (n=1) or 0.5 mL of plasmid solution containing 0.5 μg of pREGFP/dog (n=4) were injected into the myocardium of the left ventricular lateral wall. The dogs were euthanized 1 week later, and cardiac biopsies were obtained. Results - Fluorescence microscopy showed differences between the cells transfected and not transfected with pREGFP plasmid. Mild fluorescence was observed in the cardiac fibers that received saline solution; however, the myocardial cells transfected with pREGFP had overt EGFP expression. Conclusion - Transfection with the EGFP gene in healthy canine myocardium was effective. The reproduction of this efficacy using vascular endothelial growth factor (VEGF) instead of EGFP aims at developing gene therapy for ischemic heart disease. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2010-04-16T09:11:07Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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publishedVersion |
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http://hdl.handle.net/10183/19665 |
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0066-782X |
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000372497 |
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http://hdl.handle.net/10183/19665 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Arquivos brasileiros de cardiologia. São Paulo. Vol. 79, n. 3 (2002), p. 228-232 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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