Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells

Detalhes bibliográficos
Autor(a) principal: Barbosa, Fernanda Marins Costa
Data de Publicação: 2018
Outros Autores: Dupin, Talita Vieira, Toledo, Mayte Dos Santos, Reis, Natasha Ferraz Dos Campos, Ribeiro, Kleber, Cronemberger-Andrade, André, Rugani, Jeronimo Nunes, Lorenzo, Beatriz Helena Pizarro De, Brito, Ronni Rômulo Novaes e, Soares, Rodrigo Pedro Pinto, Torrecilhas, Ana Claudia, Xander, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54699
Resumo: FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO
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spelling Barbosa, Fernanda Marins CostaDupin, Talita VieiraToledo, Mayte Dos SantosReis, Natasha Ferraz Dos CamposRibeiro, KleberCronemberger-Andrade, AndréRugani, Jeronimo NunesLorenzo, Beatriz Helena Pizarro DeBrito, Ronni Rômulo Novaes eSoares, Rodrigo Pedro PintoTorrecilhas, Ana ClaudiaXander, Patricia2022-08-18T12:58:48Z2022-08-18T12:58:48Z2018BARBOSA, Fernanda Marins Costa et al. Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells. Front Microbiol., v. 9, 3056, 2018. doi: 10.3389/fmicb.2018.030561664-302Xhttps://www.arca.fiocruz.br/handle/icict/5469910.3389/fmicb.2018.03056FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOUniversidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. Diadema, SP, Brazil.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. Diadema, SP, Brazil.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. Diadema, SP, Brazil.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. Diadema, SP, Brazil.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. Diadema, SP, Brazil.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. Diadema, SP, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Centro Universitário São Camilo. São Paulo, SP, Brazil.Centro Universitário São Camilo. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. Diadema, SP, Brazil.Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. Diadema, SP, Brazil.The extracellular vesicles (EVs) released by Leishmania can contribute to the establishment of infection and host immunomodulation. In this study, we characterized the shedding of EVs from Leishmania (Leishmania) amazonensis promastigotes. This species is the causative agent of cutaneous leishmaniasis, and its role during interactions with bone marrow-derived macrophages (BMDMs) and peritoneal B-1 cells was evaluated. Leishmania amazonensis promastigotes cultivated in vitro at different times and temperatures spontaneously released EVs. EVs were purified using size-exclusion chromatography (SEC) and quantitated by nanoparticle tracking analysis (NTA). NTA revealed that the average size of the EVs was approximately 180 nm, with concentrations ranging from 1.8 x 10(8) to 2.4 x 10(9) vesicles/mL. In addition, the presence of LPG and GP63 were detected in EVs obtained at different temperatures. NaNe BMDMs stimulated with EVs exhibited increased IL-10 and IL-6 expression. However, incubating B-1 cells with parasite EVs did not stimulate IL-10 expression but led to an increase in the expression of IL-6 and TNF alpha. After 7 weeks post-infection, animals infected with L. amazonensis promastigotes in the presence of parasite EVs had significant higher parasite load and a polarization to Th2 response, as compared to the group infected with the parasite alone. This work demonstrated that EVs isolated from L. amazonensis promastigotes were able to stimulate macrophages and B-1 cells to express different types of cytokines. Moreover, the immunomodulatory properties of EVs probably contributed to an increase in parasite burden in mice. These findings suggest that the functionality of L. amazonensis EVs on immune system favor of parasite survival and disease progression.engFrontiers Research FoundationExtracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleL. amazonensisextracellular vesiclesmacrophages, B-1 cellscytokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALExtracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells.pdfExtracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells.pdfapplication/pdf2400468https://www.arca.fiocruz.br/bitstream/icict/54699/2/Extracellular%20Vesicles%20Released%20by%20Leishmania%20%28Leishmania%29%20amazonensis%20Promote%20Disease%20Progression%20and%20Induce%20the%20Production%20of%20Different%20Cytokines%20in%20Macrophages%20and%20B-1%20Cells.pdf740989fec12829dc2ca385bd56044812MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/54699/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/546992022-08-18 09:58:48.918oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-08-18T12:58:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells
title Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells
spellingShingle Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells
Barbosa, Fernanda Marins Costa
L. amazonensis
extracellular vesicles
macrophages, B-1 cells
cytokines
title_short Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells
title_full Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells
title_fullStr Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells
title_full_unstemmed Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells
title_sort Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells
author Barbosa, Fernanda Marins Costa
author_facet Barbosa, Fernanda Marins Costa
Dupin, Talita Vieira
Toledo, Mayte Dos Santos
Reis, Natasha Ferraz Dos Campos
Ribeiro, Kleber
Cronemberger-Andrade, André
Rugani, Jeronimo Nunes
Lorenzo, Beatriz Helena Pizarro De
Brito, Ronni Rômulo Novaes e
Soares, Rodrigo Pedro Pinto
Torrecilhas, Ana Claudia
Xander, Patricia
author_role author
author2 Dupin, Talita Vieira
Toledo, Mayte Dos Santos
Reis, Natasha Ferraz Dos Campos
Ribeiro, Kleber
Cronemberger-Andrade, André
Rugani, Jeronimo Nunes
Lorenzo, Beatriz Helena Pizarro De
Brito, Ronni Rômulo Novaes e
Soares, Rodrigo Pedro Pinto
Torrecilhas, Ana Claudia
Xander, Patricia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Fernanda Marins Costa
Dupin, Talita Vieira
Toledo, Mayte Dos Santos
Reis, Natasha Ferraz Dos Campos
Ribeiro, Kleber
Cronemberger-Andrade, André
Rugani, Jeronimo Nunes
Lorenzo, Beatriz Helena Pizarro De
Brito, Ronni Rômulo Novaes e
Soares, Rodrigo Pedro Pinto
Torrecilhas, Ana Claudia
Xander, Patricia
dc.subject.en.pt_BR.fl_str_mv L. amazonensis
extracellular vesicles
macrophages, B-1 cells
cytokines
topic L. amazonensis
extracellular vesicles
macrophages, B-1 cells
cytokines
description FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2022-08-18T12:58:48Z
dc.date.available.fl_str_mv 2022-08-18T12:58:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Fernanda Marins Costa et al. Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells. Front Microbiol., v. 9, 3056, 2018. doi: 10.3389/fmicb.2018.03056
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54699
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2018.03056
identifier_str_mv BARBOSA, Fernanda Marins Costa et al. Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells. Front Microbiol., v. 9, 3056, 2018. doi: 10.3389/fmicb.2018.03056
1664-302X
10.3389/fmicb.2018.03056
url https://www.arca.fiocruz.br/handle/icict/54699
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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