Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti

Detalhes bibliográficos
Autor(a) principal: Caragata, Eric Pearce
Data de Publicação: 2016
Outros Autores: Rezende, Fernanda Oliveira, Simões, Taynãna César, Moreira, Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17901
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, Minas Gerais, Brazil.
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spelling Caragata, Eric PearceRezende, Fernanda OliveiraSimões, Taynãna CésarMoreira, Luciano Andrade2017-02-24T17:51:12Z2017-02-24T17:51:12Z2016CARAGATA, Eric Pearce et al. Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti. PLoS Negl Trop Dis. V. 10, n. 11, art.e0005158, 20161935-2735https://www.arca.fiocruz.br/handle/icict/1790110.1371/journal.pntd.0005158. eCollection 2016engPublic Library of ScienceAedes aegyptiWolbachiaAedes aegyptiWolbachiaDiet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegyptiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, Minas Gerais, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, Minas Gerais, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Serviço de Apoio a Métodos Quantitativos. Belo Horizonte, Minas Gerais, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, Minas Gerais, BrazilThe pathogen interference phenotype greatly restricts infection with dengue virus (DENV) and other pathogens in Wolbachia-infected Aedes aegypti, and is a vital component of Wolbachia-based mosquito control. Critically, the phenotype's causal mechanism is complex and poorly understood, with recent evidence suggesting that the cause may be species specific. To better understand this important phenotype, we investigated the role of diet-induced nutritional stress on interference against DENV and the avian malarial parasite Plasmodium gallinaceum in Wolbachia-infected Ae. aegypti, and on physiological processes linked to the phenotype. Wolbachia-infected mosquitoes were fed one of four different concentrations of sucrose, and then challenged with either P. gallinaceum or DENV. Interference against P. gallinaceum was significantly weakened by the change in diet however there was no effect on DENV interference. Immune gene expression and H2O2 levels have previously been linked to pathogen interference. These traits were assayed for mosquitoes on each diet using RT-qPCR and the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit, and it was observed that the change in diet did not significantly affect immune expression, but low carbohydrate levels led to a loss of ROS induction in Wolbachia-infected mosquitoes. Our data suggest that host nutrition may not influence DENV interference for Wolbachia-infected mosquitoes, but Plasmodium interference may be linked to both nutrition and oxidative stress. This pathogen-specific response to nutritional change highlights the complex nature of interactions between Wolbachia and pathogens in mosquitoes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/17901/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALve_Caragata_Eric_Diet-Induced_CPqRR_2016.pdfve_Caragata_Eric_Diet-Induced_CPqRR_2016.pdfapplication/pdf13225455https://www.arca.fiocruz.br/bitstream/icict/17901/2/ve_Caragata_Eric_Diet-Induced_CPqRR_2016.pdff8dd464b9978391dc3ae30abdae73ee5MD52TEXTve_Caragata_Eric_Diet-Induced_CPqRR_2016.pdf.txtve_Caragata_Eric_Diet-Induced_CPqRR_2016.pdf.txtExtracted texttext/plain948https://www.arca.fiocruz.br/bitstream/icict/17901/3/ve_Caragata_Eric_Diet-Induced_CPqRR_2016.pdf.txt237ad11bfcf91a6481b0890a16f62687MD53icict/179012019-06-19 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dc.title.pt_BR.fl_str_mv Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti
title Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti
spellingShingle Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti
Caragata, Eric Pearce
Aedes aegypti
Wolbachia
Aedes aegypti
Wolbachia
title_short Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti
title_full Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti
title_fullStr Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti
title_full_unstemmed Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti
title_sort Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti
author Caragata, Eric Pearce
author_facet Caragata, Eric Pearce
Rezende, Fernanda Oliveira
Simões, Taynãna César
Moreira, Luciano Andrade
author_role author
author2 Rezende, Fernanda Oliveira
Simões, Taynãna César
Moreira, Luciano Andrade
author2_role author
author
author
dc.contributor.author.fl_str_mv Caragata, Eric Pearce
Rezende, Fernanda Oliveira
Simões, Taynãna César
Moreira, Luciano Andrade
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Wolbachia
topic Aedes aegypti
Wolbachia
Aedes aegypti
Wolbachia
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Wolbachia
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, Minas Gerais, Brazil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-24T17:51:12Z
dc.date.available.fl_str_mv 2017-02-24T17:51:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARAGATA, Eric Pearce et al. Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti. PLoS Negl Trop Dis. V. 10, n. 11, art.e0005158, 2016
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17901
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0005158. eCollection 2016
identifier_str_mv CARAGATA, Eric Pearce et al. Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti. PLoS Negl Trop Dis. V. 10, n. 11, art.e0005158, 2016
1935-2735
10.1371/journal.pntd.0005158. eCollection 2016
url https://www.arca.fiocruz.br/handle/icict/17901
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/17901/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/17901/2/ve_Caragata_Eric_Diet-Induced_CPqRR_2016.pdf
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