Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil

Detalhes bibliográficos
Autor(a) principal: Rahman, Rafi Ur
Data de Publicação: 2021
Outros Autores: Cosme, Luciano Veiga, Costa, Monique Melo, Carrara, Luana, Lima, Jose´ Bento Pereira, Martins, Ademir Jesus
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46525
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.
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spelling Rahman, Rafi UrCosme, Luciano VeigaCosta, Monique MeloCarrara, LuanaLima, Jose´ Bento PereiraMartins, Ademir Jesus2021-04-06T19:21:19Z2021-04-06T19:21:19Z2021RAHMAN, Rafi Ur et al. Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil. PLOS Neglected Tropical Diseases, v. 15, n. 2, e0008492, p. 1-25, Feb. 2021.1935-2727https://www.arca.fiocruz.br/handle/icict/4652510.1371/journal.pntd.00084921935-2735engPublic Library of ScienceAedes aegyptiEstrutura genéticaResistência a InseticidasRio de JaneiroBrasilInsecticide resistanceGenetic structureAedes aegyptiRio de JaneiroBrazilInsecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Yale University. Department of Ecology and Evolutionary Biology. New Haven, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Vector control largely relies on neurotoxic chemicals, and insecticide resistance (IR) directly threatens their effectiveness. In some cases, specific alleles cause IR, and knowledge of the genetic diversity and gene flow among mosquito populations is crucial to track their arrival, rise, and spread. Here we evaluated Aedes aegypti populations’ susceptibility status, collected in 2016 from six different municipalities of Rio de Janeiro state (RJ), to temephos, pyriproxyfen, malathion, and deltamethrin. We collected eggs of Ae. aegypti in Campos dos Goytacazes (Cgy), Itaperuna (Ipn), Iguaba Grande (Igg), Itaboraı´ (Ibr), Mangaratiba (Mgr), and Vassouras (Vsr). We followed the World Health Organization (WHO) guidelines and investigated the degree of susceptibility/resistance of mosquitoes to these insecticides. We used the Rockefeller strain as a susceptible positive control. We genotyped the V1016I and F1534C knockdown resistance (kdr) alleles using qPCR TaqMan SNP genotyping assay. Besides, with the use of Ae. aegypti SNP-chip, we performed genomic population analyses by genotyping more than 15,000 biallelic SNPs in mosquitoes from each population. We added previous data from populations from other countries to evaluate the ancestry of RJ populations. All RJ Ae. aegypti populations were susceptible to pyriproxyfen and malathion and highly resistant to deltamethrin. The resistance ratios for temephos was below 3,0 in Cgy, Ibr, and Igg populations, representing the lowest rates since IR monitoring started in this Brazilian region. We found the kdr alleles in high frequencies in all populations, partially justifying the observed resistance to pyrethroid. Population genetics analysis showed that Ae. aegypti revealed potential higher migration among some RJ localities and low genetic structure for most of them. Future population genetic studies, together with IR data in Ae aegypti on a broader scale, can help us predict the gene flow within and among the Brazilian States, allowing us to track the dynamics of arrival and changes in the frequency of IR alleles, and providing critical information to improving vector control program.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46525/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAdemirJMartins_LuanaCarrara_etal_IOC_2021.pdfAdemirJMartins_LuanaCarrara_etal_IOC_2021.pdfapplication/pdf3643759https://www.arca.fiocruz.br/bitstream/icict/46525/2/AdemirJMartins_LuanaCarrara_etal_IOC_2021.pdf2c5f2af97f606f51a4653ac0da53f204MD52TEXTAdemirJMartins_LuanaCarrara_etal_IOC_2021.pdf.txtAdemirJMartins_LuanaCarrara_etal_IOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil
title Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil
spellingShingle Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil
Rahman, Rafi Ur
Aedes aegypti
Estrutura genética
Resistência a Inseticidas
Rio de Janeiro
Brasil
Insecticide resistance
Genetic structure
Aedes aegypti
Rio de Janeiro
Brazil
title_short Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil
title_full Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil
title_fullStr Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil
title_full_unstemmed Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil
title_sort Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil
author Rahman, Rafi Ur
author_facet Rahman, Rafi Ur
Cosme, Luciano Veiga
Costa, Monique Melo
Carrara, Luana
Lima, Jose´ Bento Pereira
Martins, Ademir Jesus
author_role author
author2 Cosme, Luciano Veiga
Costa, Monique Melo
Carrara, Luana
Lima, Jose´ Bento Pereira
Martins, Ademir Jesus
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rahman, Rafi Ur
Cosme, Luciano Veiga
Costa, Monique Melo
Carrara, Luana
Lima, Jose´ Bento Pereira
Martins, Ademir Jesus
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Estrutura genética
Resistência a Inseticidas
Rio de Janeiro
Brasil
topic Aedes aegypti
Estrutura genética
Resistência a Inseticidas
Rio de Janeiro
Brasil
Insecticide resistance
Genetic structure
Aedes aegypti
Rio de Janeiro
Brazil
dc.subject.en.pt_BR.fl_str_mv Insecticide resistance
Genetic structure
Aedes aegypti
Rio de Janeiro
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-04-06T19:21:19Z
dc.date.available.fl_str_mv 2021-04-06T19:21:19Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAHMAN, Rafi Ur et al. Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil. PLOS Neglected Tropical Diseases, v. 15, n. 2, e0008492, p. 1-25, Feb. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46525
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0008492
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv RAHMAN, Rafi Ur et al. Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil. PLOS Neglected Tropical Diseases, v. 15, n. 2, e0008492, p. 1-25, Feb. 2021.
1935-2727
10.1371/journal.pntd.0008492
1935-2735
url https://www.arca.fiocruz.br/handle/icict/46525
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/46525/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/46525/2/AdemirJMartins_LuanaCarrara_etal_IOC_2021.pdf
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