Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation

Detalhes bibliográficos
Autor(a) principal: Reis, Maria Danielma dos Santos
Data de Publicação: 2015
Outros Autores: Csomos, Krisztian, Dias, Luciene Paschoal Braga, Prodan, Zsolt, Szerafin, Tamas, Savino, Wilson, Takacs, Laszlo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13186
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / University of Debrecen. Faculty of Medicine. Department of Human Genetics. Debrecen, Hungary.
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spelling Reis, Maria Danielma dos SantosCsomos, KrisztianDias, Luciene Paschoal BragaProdan, ZsoltSzerafin, TamasSavino, WilsonTakacs, Laszlo2016-03-16T13:31:11Z2016-03-16T13:31:11Z2015REIS, Maria Danielma dos Santos; et al. Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation. Immunity & Ageing, v.12:18, 14p, 2015.1742-4933https://www.arca.fiocruz.br/handle/icict/1318610.1186/s12979-015-0045-9engBioMed CentralDecline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / University of Debrecen. Faculty of Medicine. Department of Human Genetics. Debrecen, Hungary.University of Debrecen. Faculty of Medicine. Department of Biochemistry and Molecular Biology. Debrecen, Hungary.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Gottsegen György Hungarian Institute of Cardiology. Budapest, Hungary.University of Debrecen. Clinical Center. Department of Cardiac Surgery. Debrecen, Hungary.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.University of Debrecen. Faculty of Medicine. Department of Human Genetics. Debrecen, Hungary / Biosystems International Kft. Debrecen, Hungary.Background: Thymic involution is thought to be an important factor of age related immunodeficiency. Understanding the molecular mechanisms of human thymic senescence may lead to the discovery of novel therapeutic approaches aimed at the reestablishment of central and peripheral T cell repertoire. Results: As an initial approach, here we report that the decline of human thymic FOXN1 transcription correlates with age, while other genes, DLL1, DLL4 and WNT4, essential for thymopoiesis, are constitutively transcribed. Using a human thymic epithelial cell line (hTEC), we show that FOXN1 expression is refractory to signals that induce FOXN1 transcription in primary 3D culture conditions and by stimulation of the canonical WNT signaling pathway. Blockage of FOXN1 induceability in the hTEC line may be mediated by an epigenetic mechanism, the CpG methylation of the FOXN1 gene. Conclusion: We showed a suppression of FOXN1 transcription both in cultured human thymic epithelial cells and in the aging thymus. We hypothesize that the underlying mechanism may be associated with changes of the DNA methylation state of the FOXN1 gene.ThymusFOXN1 gene expressionEpigenetic regulationTimoExpressão GênicaEpigênese Genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13186/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALwilson2_savino_etal_IOC_2015.pdfwilson2_savino_etal_IOC_2015.pdfapplication/pdf3767781https://www.arca.fiocruz.br/bitstream/icict/13186/2/wilson2_savino_etal_IOC_2015.pdf43a744d0d5a5f9402ebc023d3834a8aeMD52TEXTwilson2_savino_etal_IOC_2015.pdf.txtwilson2_savino_etal_IOC_2015.pdf.txtExtracted texttext/plain61046https://www.arca.fiocruz.br/bitstream/icict/13186/3/wilson2_savino_etal_IOC_2015.pdf.txt2b4a2494adc686c6a8c76322e6fd8601MD53icict/131862023-09-05 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dc.title.pt_BR.fl_str_mv Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
title Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
spellingShingle Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
Reis, Maria Danielma dos Santos
Thymus
FOXN1 gene expression
Epigenetic regulation
Timo
Expressão Gênica
Epigênese Genética
title_short Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
title_full Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
title_fullStr Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
title_full_unstemmed Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
title_sort Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation
author Reis, Maria Danielma dos Santos
author_facet Reis, Maria Danielma dos Santos
Csomos, Krisztian
Dias, Luciene Paschoal Braga
Prodan, Zsolt
Szerafin, Tamas
Savino, Wilson
Takacs, Laszlo
author_role author
author2 Csomos, Krisztian
Dias, Luciene Paschoal Braga
Prodan, Zsolt
Szerafin, Tamas
Savino, Wilson
Takacs, Laszlo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Reis, Maria Danielma dos Santos
Csomos, Krisztian
Dias, Luciene Paschoal Braga
Prodan, Zsolt
Szerafin, Tamas
Savino, Wilson
Takacs, Laszlo
dc.subject.en.pt_BR.fl_str_mv Thymus
FOXN1 gene expression
Epigenetic regulation
topic Thymus
FOXN1 gene expression
Epigenetic regulation
Timo
Expressão Gênica
Epigênese Genética
dc.subject.decs.pt_BR.fl_str_mv Timo
Expressão Gênica
Epigênese Genética
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / University of Debrecen. Faculty of Medicine. Department of Human Genetics. Debrecen, Hungary.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-16T13:31:11Z
dc.date.available.fl_str_mv 2016-03-16T13:31:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REIS, Maria Danielma dos Santos; et al. Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation. Immunity & Ageing, v.12:18, 14p, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13186
dc.identifier.issn.none.fl_str_mv 1742-4933
dc.identifier.doi.none.fl_str_mv 10.1186/s12979-015-0045-9
identifier_str_mv REIS, Maria Danielma dos Santos; et al. Decline of FOXN1 gene expression in human thymus correlates with age: possible epigenetic regulation. Immunity & Ageing, v.12:18, 14p, 2015.
1742-4933
10.1186/s12979-015-0045-9
url https://www.arca.fiocruz.br/handle/icict/13186
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/13186/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/13186/2/wilson2_savino_etal_IOC_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/13186/3/wilson2_savino_etal_IOC_2015.pdf.txt
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