Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis

Detalhes bibliográficos
Autor(a) principal: Lyra, Andre C.
Data de Publicação: 2003
Outros Autores: Pinho, João R. R., Mello, Isabel M. V. G. C., Silva, Luciano Kalabric, Braga, Eduardo L., Lyra, Marcos C., Oliveira, Maxuel, Silva, Luiz C. da, Carrilho, Flair J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44313
Resumo: 0 objetivo desse estudo foi avaliar a prevalencia da infeccao pelo TTV em pacientes com hepatite aguda A e B, e genotipar os isolados do TTV atraves de analise filogendtica. Foram avaliados soros de 82 pacientes que apresentaram hepatite aguda A (N = 40) e B (N = 42), e 71 doadores de sangue. 0 TTV foi deterrninado atraves "nested-PCR", e a analise filogendtica foi realizada utilizando o mdtodo "neighborj oining': 0 TTV foi detectado ern 23% dos pacientes corn hepatite aguda e em 31% dos doadores. Os niveis medios de amino- transferases foram semelhantes em pacientes TTV positivos e pacientes TTV negativos. Uma arvore filogend- tica foi construida e mostrou isolados do TTV dos genOtipos 1, 2, 3 e 4. Em conclusk, a infeccao pelo TTV foi mais freq0ente entre doadores de sangue do que em pacientes corn hepatite aguda A ou B, em Salvador- Bahia. 0 TTV nao aumentou a gravidade da atividade necroinflamatdria dos pacientes com hepatite aguda A ou B. Atraves de analise filogendtica foram encontrados isolados do TTV dos genotipos 1, 2, 3 e 4 na popu- lack estudada.
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spelling Lyra, Andre C.Pinho, João R. R.Mello, Isabel M. V. G. C.Silva, Luciano KalabricBraga, Eduardo L.Lyra, Marcos C.Oliveira, MaxuelSilva, Luiz C. daCarrilho, Flair J.2020-11-05T12:28:59Z2020-11-05T12:28:59Z2003LYRA, André C. et al. Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis. GED, v. 22, n. 4, p. 129-132, 2003.https://www.arca.fiocruz.br/handle/icict/443130 objetivo desse estudo foi avaliar a prevalencia da infeccao pelo TTV em pacientes com hepatite aguda A e B, e genotipar os isolados do TTV atraves de analise filogendtica. Foram avaliados soros de 82 pacientes que apresentaram hepatite aguda A (N = 40) e B (N = 42), e 71 doadores de sangue. 0 TTV foi deterrninado atraves "nested-PCR", e a analise filogendtica foi realizada utilizando o mdtodo "neighborj oining': 0 TTV foi detectado ern 23% dos pacientes corn hepatite aguda e em 31% dos doadores. Os niveis medios de amino- transferases foram semelhantes em pacientes TTV positivos e pacientes TTV negativos. Uma arvore filogend- tica foi construida e mostrou isolados do TTV dos genOtipos 1, 2, 3 e 4. Em conclusk, a infeccao pelo TTV foi mais freq0ente entre doadores de sangue do que em pacientes corn hepatite aguda A ou B, em Salvador- Bahia. 0 TTV nao aumentou a gravidade da atividade necroinflamatdria dos pacientes com hepatite aguda A ou B. Atraves de analise filogendtica foram encontrados isolados do TTV dos genotipos 1, 2, 3 e 4 na popu- lack estudada.The aim of this study was to evaluate the prevalence of TTV in patients with acute hepatitis A and B, and to genotype TTV isolates by phylogenetic analysis. The authors evaluated sera of 82 patients who had acute hepatitis A (N = 40) and B (N = 42), and of 71 blood donors. TTV was detected by nested-PCR assay and phylogenetic analysis was performed using the neighbor-joining method. TTV was detected in 23% of pa- tients with acute hepatitis and in 31% of donors. Mean aminotransferase levels were similar in patients who were TTV positive and TTV negative. A phylogenetic tree was drawn that showed TTV isolates of genotype 1, 2, 3, and 4. In conclusion, TTV infection is more frequent among blood donors than among patients with acute hepatitis A or B in Salvador-Bahia. TTV does not appear to increase the severity of necroinflammatory activity of acute hepatitis A or B. Using phylogenetic analysis TTV isolates of genotype 1, 2, 3, and 4 were encountered in the study population.Alves de Queiroz Family Fund for Research.University of São Paulo. School of Medicine. Department of Gastroenterology. São Paulo, SP, Brazil.University of São Paulo. School of Medicine. Department of Gastroenterology. São Paulo, SP, Brazil.Institute Adolpho Lutz. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Institute Adolpho Lutz. São Paulo, SP, Brazil.Federal University of Bahia. Division of Gastro-Hepatology. Salvador, BA, Brazil.Federal University of Bahia. Division of Gastro-Hepatology. Salvador, BA, Brazil.Federal University of Bahia. Division of Gastro-Hepatology. Salvador, BA, Brazil.University of São Paulo. School of Medicine. Department of Gastroenterology. São Paulo, SP, Brazil.engHepatite agudaTTVGenótipoAnálise filogenéticaAcute hepatitisTTVGenotypePhylogenetic analysisPrevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/44313/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALLyra Andre C Prevalence....pdfLyra Andre C Prevalence....pdfapplication/pdf5239188https://www.arca.fiocruz.br/bitstream/icict/44313/2/Lyra%20Andre%20C%20Prevalence....pdf3863cad7ad2cb1e45b02d220fd129b2bMD52TEXTLyra Andre C Prevalence....pdf.txtLyra Andre C Prevalence....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis
title Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis
spellingShingle Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis
Lyra, Andre C.
Hepatite aguda
TTV
Genótipo
Análise filogenética
Acute hepatitis
TTV
Genotype
Phylogenetic analysis
title_short Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis
title_full Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis
title_fullStr Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis
title_full_unstemmed Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis
title_sort Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis
author Lyra, Andre C.
author_facet Lyra, Andre C.
Pinho, João R. R.
Mello, Isabel M. V. G. C.
Silva, Luciano Kalabric
Braga, Eduardo L.
Lyra, Marcos C.
Oliveira, Maxuel
Silva, Luiz C. da
Carrilho, Flair J.
author_role author
author2 Pinho, João R. R.
Mello, Isabel M. V. G. C.
Silva, Luciano Kalabric
Braga, Eduardo L.
Lyra, Marcos C.
Oliveira, Maxuel
Silva, Luiz C. da
Carrilho, Flair J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lyra, Andre C.
Pinho, João R. R.
Mello, Isabel M. V. G. C.
Silva, Luciano Kalabric
Braga, Eduardo L.
Lyra, Marcos C.
Oliveira, Maxuel
Silva, Luiz C. da
Carrilho, Flair J.
dc.subject.other.pt_BR.fl_str_mv Hepatite aguda
TTV
Genótipo
Análise filogenética
topic Hepatite aguda
TTV
Genótipo
Análise filogenética
Acute hepatitis
TTV
Genotype
Phylogenetic analysis
dc.subject.en.pt_BR.fl_str_mv Acute hepatitis
TTV
Genotype
Phylogenetic analysis
description 0 objetivo desse estudo foi avaliar a prevalencia da infeccao pelo TTV em pacientes com hepatite aguda A e B, e genotipar os isolados do TTV atraves de analise filogendtica. Foram avaliados soros de 82 pacientes que apresentaram hepatite aguda A (N = 40) e B (N = 42), e 71 doadores de sangue. 0 TTV foi deterrninado atraves "nested-PCR", e a analise filogendtica foi realizada utilizando o mdtodo "neighborj oining': 0 TTV foi detectado ern 23% dos pacientes corn hepatite aguda e em 31% dos doadores. Os niveis medios de amino- transferases foram semelhantes em pacientes TTV positivos e pacientes TTV negativos. Uma arvore filogend- tica foi construida e mostrou isolados do TTV dos genOtipos 1, 2, 3 e 4. Em conclusk, a infeccao pelo TTV foi mais freq0ente entre doadores de sangue do que em pacientes corn hepatite aguda A ou B, em Salvador- Bahia. 0 TTV nao aumentou a gravidade da atividade necroinflamatdria dos pacientes com hepatite aguda A ou B. Atraves de analise filogendtica foram encontrados isolados do TTV dos genotipos 1, 2, 3 e 4 na popu- lack estudada.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2020-11-05T12:28:59Z
dc.date.available.fl_str_mv 2020-11-05T12:28:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv LYRA, André C. et al. Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis. GED, v. 22, n. 4, p. 129-132, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44313
identifier_str_mv LYRA, André C. et al. Prevalence of TTV in blood donors and patients with acute viral hepatitis and genotyping by phylogenetic analysis. GED, v. 22, n. 4, p. 129-132, 2003.
url https://www.arca.fiocruz.br/handle/icict/44313
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